Biologia plantarum 57:675-683, 2013 | DOI: 10.1007/s10535-013-0346-9

Peroxiredoxins are involved in two independent signalling pathways in the abiotic stress protection in Vitis vinifera

P. Vidigal1, R. Carvalho1, S. Amâncio1, L. Carvalho1,*
1 DRAT/CBAA, Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Lisboa, Portugal

Peroxiredoxins (Prxs) play major roles in preventing oxidative damage and their function is consistent with the presence of Prx isoforms in most, if not all, cellular compartments and their expression is dependent on environmental conditions. The aim of this study was to identify and characterize genes encoding Prxs in Vitis vinifera. Quantitative real time polymerase chain reaction (qRT PCR) was used to determine their response to irradiance, heat, and water stress. We identified seven vvprx genes, two of which were especially responsive to water stress, followed by heat stress, but no major changes were observed after high irradiance. The vvprxIIF targeted to mitochondria was the most responsive to water stress and it might be involved in drought tolerance through H2O2 signalling. The vvprxII-2, a putative PrxII, is targeted to the chloroplasts and was the most responsive to heat stress. It might be related with abscisic acid-dependent thermotolerance. Additional key words: abscisic acid, antioxidants, grapevine, heat stress, photosynthesis, qRT PCR, water stress.

Subjects: peroxiredoxins; abscisic acid; antioxidants; temperature - high; water stress; catalase; ascorbate peroxidase; superoxide dismutase; glutathione reductase; net photosynthetic rate; chlorophyll fluorescence; grapevine

Received: November 20, 2012; Accepted: March 21, 2013; Published: December 1, 2013Show citation

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Vidigal, P., Carvalho, R., Amâncio, S., & Carvalho, L. (2013). Peroxiredoxins are involved in two independent signalling pathways in the abiotic stress protection in Vitis vinifera. Biologia plantarum, 57(4), 675-683. doi: 10.1007/s10535-013-0346-9.
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