Biologia plantarum 60:783-787, 2016 | DOI: 10.1007/s10535-016-0603-9

Enhancement of antioxidant enzyme activities in rice callus by ascorbic acid under salinity stress

A. N. Alhasnawi1,2,*, C. M. Z. Che Radziah1, A. A. Kadhimi1,3, A. Isahak1, A. Mohamad4, W. M. W. Yusoff1
1 Faculty of Science and Technology, University of Kebangsaan, UKM, Bangim, Malaysia
2 University Presidency, AL-Muthanna University, Samawah, Iraq
3 University of Baghdad, Ministry of Higher Education, Jadriyah, Baghdad, Iraq
4 Malaysian Nuclear Agency, Kajang, Bangi, Malaysia

Ascorbic acid (AsA) is naturally occurring compound with antioxidant activity and plays a pivotal role in plant cell adaptation to salinity stress. The objective of this work was to assess the influence of exogenous AsA on the embryogenic callus of indica rice (Oryza sativa L.) cv. MRQ74 cultivated under saline conditions. NaCl (200 mM) decreased callus fresh and dry masses, relative growth rate, and K+ and Ca+2 content, and increased Na+ content and Na+/K+ ratio. Application of AsA (0.5 or 1 mM) alleviated these effects of salinity. Activities of peroxidase, catalase, superoxide dismutase, as well as content of proline increased due to the NaCl treatment, and these parameters were mostly further increased by 0.5 mM AsA. Thus, AsA can increase callus tolerance to NaCl stress.

Keywords: catalase; embryogenic callus; peroxidase; proline; salt tolerance; superoxide dismutase
Subjects: antioxidative enzymes; ascorbic acid; catalase; peroxidase; superoxide dismutase; proline; in vitro culture; sodium; potassium; calcium; rice

Received: September 14, 2015; Revised: December 29, 2015; Accepted: January 18, 2016; Published: December 1, 2016Show citation

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Alhasnawi, A.N., Radziah, C.M.Z., Kadhimi, A.A., Isahak, A., Mohamad, A., & Yusoff, W.M.W. (2016). Enhancement of antioxidant enzyme activities in rice callus by ascorbic acid under salinity stress. Biologia plantarum, 60(4), 783-787. doi: 10.1007/s10535-016-0603-9.
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