Biologia plantarum 62:211-221, 2018 | DOI: 10.1007/s10535-018-0781-8

Anatomy and photosystem II activity of in vitro grown Aechmea blanchetiana as affected by 1-naphthaleneacetic acid

J. P. R. Martins1,*, L. C. A. Rodrigues2, E. R. Santos1, B. G. Batista2, A. B. P. L. Gontijo2, A. R. Falqueto1
1 Plant Ecophysiology Laboratory, Federal University of Espírito Santo, Litorâneo, São Mateus, Brazil
2 Plant Tissue Culture Laboratory, Federal University of Espírito Santo, Litorâneo, São Mateus, Brazil

Auxins are one of the main regulators of in vitro plant growth and development. However, the mechanisms, by which auxins, such as 1-naphthaleneacetic acid (NAA), affect in vitro root and leaf anatomy and photosystem function, remain unclear. Accordingly, the aim of the present study was to analyze the effect of different NAA concentrations on the anatomy and photosynthetic performance of in vitro-propagated Aechmea blanchetiana and to determine whether such a treatment affects micropropagated plants after acclimatization. In vitro-established A. blanchetiana plants were transferred to culture media that contained 0, 2, 4, or 6 μM NAA, and after 50 d, they were transplanted into plastic seedling trays with a commercial substrate and cultivated for 60 d in a greenhouse. The plants were evaluated after a 50-d in vitro NAA exposure (growth traits, chlorophyll α fluorescence, and root and leaf anatomy) and after 60 d of acclimatization in the greenhouse (root and leaf growth). Changes induced by NAA in root anatomy might improve uptake of minerals and sugars from the medium, thereby increasing the in vitro growth. In the leaves, the lowest chlorenchyma thickness and sclerenchyma area were observed in plants grown without NAA, and NAA exposure also improved photosystem II activity. The highest ex vitro growth rate was observed for plants that were propagated with 4 μM NAA. Therefore, the use of NAA during in vitro propagation can improve the anatomical and physiological quality of A. blanchetiana plants, as well as to improve ex vitro transfer.

Keywords: auxins; chlorophyll fluorescence; photosynthetic apparatus
Subjects: in vitro culture; leaf and root anatomy; photosystem 2; chlorophyll a fluorescence; naphthaleneacetic acid
Species: Aechmea blanchetiana

Received: June 23, 2017; Revised: August 21, 2017; Accepted: September 27, 2017; Published: June 1, 2018Show citation

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Martins, J.P.R., Rodrigues, L.C.A., Santos, E.R., Batista, B.G., Gontijo, A.B.P.L., & Falqueto, A.R. (2018). Anatomy and photosystem II activity of in vitro grown Aechmea blanchetiana as affected by 1-naphthaleneacetic acid. Biologia plantarum, 62(2), 211-221. doi: 10.1007/s10535-018-0781-8.
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