Biologia plantarum 55:153-158, 2011 | DOI: 10.1007/s10535-011-0021-y

Protein changes during programmed cell death in tobacco

I. Chaves1, M. Alves1, D. Carrilho1, M. C. Duque-Magalhães1,2, C. P. Ricardo1,2,*, A. P. Regalado1
1 Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal
2 Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Lisboa, Portugal

Programmed cell death (PCD) was induced by the Yariv reagent in Nicotiana tabacum cv. Bright Yellow-2 cell suspension. The analyses of proteins extracts by 2-D electrophoresis clearly show massive protein degradation which was mainly due to cysteine protease activity. In contrast, some proteins remained unchanged up to 72 h after PCD induction. Peptide mass fingerprints of these proteins, obtained by MALDI-TOF, identified calreticulin, heat shock protein (HSP) 60, HSP70, malate dehydrogenase and mitochondrial ATP synthase β-subunit.

Keywords: heat shock proteins; calreticulin; Nicotiana tabacum BY-2 suspension cells; Yariv reagent

Received: February 15, 2009; Accepted: December 5, 2009; Published: March 1, 2011Show citation

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Chaves, I., Alves, M., Carrilho, D., Duque-Magalhães, M.C., Ricardo, C.P., & Regalado, A.P. (2011). Protein changes during programmed cell death in tobacco. Biologia plantarum, 55(1), 153-158. doi: 10.1007/s10535-011-0021-y.
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