Biologia Plantarum 63: 210-218, 2019 | DOI: 10.32615/bp.2019.024

The gene OsIAA9 encoding auxin/indole-3-acetic acid proteins is a negative regulator of auxin-regulated root growth in rice

Y.L. Song*
Anhui Key Laboratory for Biodiversity Research and Ecological Conservation on Southwest Anhui, College of Life
Science, Anqing Normal University, Anqing, 246011, P.R. China

Auxin signal transduction in plants depends on regulation by short-lived nuclear plant proteins called auxin/indole-3-acetic acid (Aux/IAA) proteins. The OsIAA9, which is highly and rapidly induced by auxin, encodes the entire Aux/IAA domain characteristic of the Aux/IAA family in rice. The promoter region of OsIAA9 contains several cis-elements. Analysis of POsIAA9:GUS transgenic plants indicates a specific expression of OsIAA9 in roots and seedling shoots, especially lateral roots and root tips. Based on real-time PCR, the expression of OsIAA9 was induced by multiple hormones and abiotic stresses. Furthermore, ectopic overexpression of OsIAA9 in rice led to fewer crown and lateral roots and reduced the inhibition of root elongation by auxin. These observations indicate that OsIAA9 was a negative regulator of auxin-regulated root growth.

Keywords: abscisic acid, crown and lateral roots, gibberelic acid, jasmonates, kinetin, Oryza sativa, salicylic acid, transgenic plants

Accepted: November 15, 2018; Prepublished online: November 15, 2018; Published online: January 19, 2019Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Song, Y.L. (2019). The gene OsIAA9 encoding auxin/indole-3-acetic acid proteins is a negative regulator of auxin-regulated root growth in rice. Biologia plantarum, 63, 210-218. doi: 10.32615/bp.2019.024.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files

    Download fileSONG5348Suppl.pdf

    File size: 396.6 kB

    References

    1. De Smet, I., Tetsumura, T., De Rybel, B., Frey, N.F., Laplaze, L., Casimiro, I., Swarup, R., Naudts, M., Vanneste, S., Audenaert, D., Inze, D., Bennett, M.J., Beeckman, T.: Auxin-dependent regulation of lateral root positioning in the basal meristem of Arabidopsis. - Development 134: 681-690, 2007. Go to original source...
    2. De Smet, I., Vanneste, S., Inze, D., Beeckman, T.: Lateral root initiation or the birth of a new meristem. - Plant mol. Biol. 60: 871-887, 2006. Go to original source...
    3. Galvan-Ampudia, C.S., Testerink, C.: Salt stress signals shape the plant root. - Curr. Opin. Plant Biol. 14: 296-302, 2011. Go to original source...
    4. Higo, K., Ugawa, Y., Iwamoto, M., Korenaga, T.: Plant cis-acting regulatory DNA elements (PLACE) database: 1999. - Nucleic Acids Res. 27: 297-300,1999. Go to original source...
    5. Hoffmann, M., Hentrich, M., Pollmann, S.: Auxin-oxylipin crosstalk: relationship of antagonists. - J. integr. Plant Biol. 53: 429-445, 2011. Go to original source...
    6. Jain, M., Kaur, N., Garg, R., Thakur, J.K., Tyagi, A.K., Khurana, J.P.: Structure and expression analysis of early auxin-responsive Aux/IAA gene family in rice (Oryza sativa). - Funct. integr. Genomics 6: 47-59, 2006. Go to original source...
    7. Jenik, P.D., Barton, M.K.: Surge and destroy: the role of auxin in plant embryogenesis. - Development 132: 3577-3585, 2005. Go to original source...
    8. Kitomi, Y., Inahashi, H., Takehisa, H., Sato, Y., Inukai, Y.: OsIAA13-mediated auxin signaling is involved in lateral root initiation in rice. - Plant Sci. 190: 116-122, 2012. Go to original source...
    9. Luo, S., Li, Q., Liu, S., Pinas, N.M., Tian, H., Wang, S.: Constitutive expression of OsIAA9 affects starch granules accumulation and root gravitropic response in Arabidopsis. - Front. Plant Sci. 6: 1156, 2015. Go to original source...
    10. Leyser, O.: The fall and rise of apical dominance. - Curr. Opin. Genet. Dev. 15: 468-471, 2005. Go to original source...
    11. Muday, G.K., DeLong, A.: Polar auxin transport: controlling where and how much. - Trends Plant Sci. 6: 535-542, 2001. Go to original source...
    12. Munns, R., Tester, M.: Mechanisms of salinity tolerance. - Annu. Rev. Plant Biol. 59: 651-681, 2008. Go to original source...
    13. Ni, J., Wang, G.H., Zhu, Z.X., Zhang, H.H., Wu, Y.R., Wu, P.: OsIAA23-mediated auxin signaling defines postembryonic maintenance of QC in rice. - Plant J. 68: 433-442, 2011.
    14. Peer, W.A., Blakeslee, J.J., Yang, H.B., Murphy, A.S.: Seven things we think we know about auxin transport. - Mol. Plant. 4: 487-504, 2011. Go to original source...
    15. Peret, B., De Rybel, B., Casimiro, I., Benkova, E., Swarup, R., Laplaze, L., Beeckman, T., Bennett, M.J.: Arabidopsis lateral root development: an emerging story. - Trends Plant Sci. 14: 399-408, 2009. Go to original source...
    16. Rebouillat, A., Dievart, J., Verdeil, J., Escoute, J., Giese, G., Breitler, J.C., Gantet, P., Espeout, S., Guiderdoni, E., Perin, C.: Molecular genetics of rice root development. - Rice 2: 15-34, 2009. Go to original source...
    17. Song, Y.L.: Insight into the mode of action of 2,4-dichlorophenoxyacetic acid (2,4-D) as an herbicide. - J. integr. Plant Biol. 56:106-13, 2014. Go to original source...
    18. Song, Y.L., Wang, L., Xiong, L.Z.: Comprehensive expression profiling analysis of OsIAA gene family in developmental processes and in response to phytohormone and stress treatments. - Planta 229: 577-591, 2009a. Go to original source...
    19. Song, Y.L., Xu, Z.F.: Ectopic overexpression of an AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) gene OsIAA4 in rice induces morphological changes and reduces responsiveness to auxin. - Int. J. mol. Sci. 14: 13645-13656, 2013. Go to original source...
    20. Song, Y.L., You, J., Xiong, L.Z.: Characterization of OsIAA1 gene, a member of rice Aux/IAA family involved in auxin and brassinosteroid hormone responses and plant morphogenesis. - Plant mol. Biol. 70: 297-309, 2009b. Go to original source...
    21. Tiwari, S.B., Hagen, G., Guilfoyle, T.J.: Aux/IAA proteins contain a potent transcriptional repression domain. - Plant Cell 16: 533-543, 2004. Go to original source...
    22. Wang, L., Xie, W.B., Chen, Y., Tang, W.J., Yang, J.Y., Ye, R.J., Liu, L., Lin, Y.J., Xu, C.G., Xiao, J.H., Zhang, Q.F.: A dynamic gene expression atlas covering the entire life cycle of rice. - Plant J. 61: 752-766, 2010. Go to original source...
    23. Wolters, H., Jurgens, G.: Survival of the flexible: hormonal growth control and adaptation in plant development. - Nat. Rev. Genet. 10: 305-317, 2009. Go to original source...
    24. Xiong, L.M., Zhu, J.K.: Abiotic stress signal transduction in plants: molecular and genetic perspectives. - Physiol. Plant. 112: 152-166, 2001. Go to original source...
    25. Zhu, Z.X., Liu, Y., Liu, S.J., Mao, C.Z., Wu, Y.R., Wu, P.: A gain-of-function mutation in OsIAA11 affects lateral root development in rice. - Mol. Plant. 5: 154-161, 2012. Go to original source...

    Return to the content