Biologia plantarum 45:91-97, 2002 | DOI: 10.1023/A:1015100624229

Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane

R.F. Fornazier1, R.R. Ferreira1, A.P. Vitória2, S.M.G. Molina1, P.J. Lea3, R.A. Azevedo1
1 Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba CEP, Brazil
2 Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Feira de Santana CEP, Brazil
3 Department of Biological Sciences, University of Lancaster, Lancaster, UK

Sugar cane (Saccharum officinarum L. cv. Copersucar SP80-3280) seedlings were grown in nutrient solution with varying concentrations (0, 2 and 5 mM) of cadmium chloride for 96 h. Leaves were analysed for catalase (CAT), glutathione reductase (GR) and superoxide dismutase (SOD) activities. Although a clear effect of CdCl2 on plant growth was observed, the activity of SOD was not altered significantly. However, the CAT activity decreased as the concentration of CdCl2 increased. GR exhibits a significant increase in activity at 2 and 5 mM CdCl2. CAT and SOD isoenzymes were further characterised by analysis in non-denaturing PAGE. Activity staining for SOD revealed up to seven isoenzymes in untreated control and 2 mM CdCl2 treated plants, corresponding to Cu/Zn-SOD isoenzymes. At 5 mM CdCl2, only six Cu/Zn-SOD isoenzymes were observed. No Fe-SOD and Mn-SOD isoenzymes were detected. For CAT, one band of activity was observed.

Keywords: catalase; glutathione reductase; heavy metals; phytochelatins; reactive oxygen species; Saccharum officinarum; superoxide dismutase
Subjects: antioxidants; cadmium, antioxidants; catalase; glutathione reductase; heavy metals; reactive oxygen species; Saccharum officinarum; sugar cane, cadmium, antioxidant enzymes; superoxide dismutase, cadmium

Published: March 1, 2002Show citation

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Fornazier, R.F., Ferreira, R.R., Vitória, A.P., Molina, S.M.G., Lea, P.J., & Azevedo, R.A. (2002). Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane. Biologia plantarum, 45(1), 91-97. doi: 10.1023/A:1015100624229.
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