Biologia plantarum 45:129-132, 2002 | DOI: 10.1023/A:1015177330589

Direct Organogenesis from Mature Leaf and Petiole Explants of Eryngium Foetidum L.

S. Arockiasamy1, S. Prakash1, S. Ignacimuthu1
1 Entomology Research Institute, Loyola College, Chennai, India

Eryngium foetidum L. plants were regenerated from mature leaf and petiole explants through direct organogenesis without intervening callus phase. From leaf explants, adventitious multiple shoots raised on Murashige and Skoog (MS) medium supplemented with 4.43 μM benzylaminopurine (BAP) and 0.57 μM indole-3-acetic acid (IAA), whereas in petiole explants shoot regeneration occurred at 8.86 μM BAP and 0.57 μM IAAA. 80% of the leaf explants and 44% of petiole explants produced shoots after four weeks of culture. The regenerated plants were rooted on MS medium supplemented with 2.46 μM indole-3-butyric acid and 2.88 μM gibberellic acid. The plants were successfully established in the soil and showed 70.9% survival in the field.

Keywords: growth regulations; in vitro cultivation; regeneration
Subjects: auxins; cytokinins; Eryngium foetidum; in vitro culture, plant regeneration, organogenesis; in vitro culture, somatic embryogenesis; organogenesis, mature leaf, petiole explant; somatic embryogenesis

Published: March 1, 2002Show citation

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Arockiasamy, S., Prakash, S., & Ignacimuthu, S. (2002). Direct Organogenesis from Mature Leaf and Petiole Explants of Eryngium Foetidum L. Biologia plantarum, 45(1), 129-132. doi: 10.1023/A:1015177330589.
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