Biologia plantarum - Latest articles

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Changes of cytosine methylation in pecan tissues of different stages by quantitative methylation-sensitive amplified polymorphismOriginal Papers

Z.Z. LIU, F. ZHOU, J. SHANG, F.R. PENG, Z.H. MO, Y.R. LI

Biologia plantarum 64: 473-484, 2020 | DOI: 10.32615/bp.2020.066

Cytosine methylation plays an important role in plant development by regulating gene expressions. However, few studies have investigated methylation changes during the tissue differentiation and development of perennial plants. Here, the fluorescence-labeled methylation-sensitive amplified polymorphism method was used with eight primer combinations to detect methylation in leaves and xylem obtained at the stages of inflorescence emergence (IE), ovary start growth, and fruit maturity (FM) in two pecan (Carya illinoinensis) cvs. Pawnee and Stuart. The results show that the total methylation in the xylem was generally higher than in the leaves...

Transcriptome analysis deciphers the mechanisms of exogenous nitric oxide action on the response of melon leaves to chilling stressSpecial Issue: Gasotransmitters

Q. DIAO, Y. CAO, H. FAN, Y. ZHANG

Biologia plantarum 64: 465-472, 2020 | DOI: 10.32615/bp.2020.021

Chilling stress is a major abiotic factor that limits the growth and productivity of melon (Cucumis melo L.). The application of nitric oxide (NO) can enhance plant tolerance to chilling stress; however, the underlying molecular mechanisms for this process remain poorly understood. In this study, RNA sequencing was performed on melon seedlings exposed to control conditions, chilling stress, or chilling stress in the presence of NO donor sodium nitroprusside (SNP), to identify NO-mediated transcript changes in response to chilling stress. The results identified 488, 1 012, and 1 589 differentially expressed genes (DEGs) between plants in optimum...

The rice Aux/IAA transcription factor gene OsIAA18 enhances salt and osmotic tolerance in ArabidopsisOriginal Papers

G. LI, Y.X. YE, X.Q. REN, M.Y. QI, H.Y. ZHAO, Q. ZHOU, X.H. CHEN, J. WANG, C.Y. YUAN, F.B. WANG

Biologia plantarum 64: 454-464, 2020 | DOI: 10.32615/bp.2019.069

In plants, auxin/indoleacetic acid (Aux/IAA) proteins are transcriptional regulators, which regulate developmental process and responses to phytohormones and stress treatments. A previous study has shown that the rice Aux/IAA transcription factor gene OsIAA18 is induced by salt and osmotic stresses. However, little is known about the regulatory functions of this gene. In this study, the OsIAA18 gene was successfully cloned from rice. Subcellular localization analysis in onion epidermal cells indicated that OsIAA18 was localized to the nucleus. Expression analysis in yeast showed that the full length OsIAA18 exhibited transcriptional activation....

Leaf nutrient homeostasis and maintenance of photosynthesis integrity contribute to adaptation of the pea mutant SGECd^t to cadmiumOriginal Papers

A.A. BELIMOV, I.C. DODD, V.I. SAFRONOVA, K.-J. DIETZ

Biologia plantarum 64: 447-453, 2020 | DOI: 10.32615/bp.2020.061

Cadmium (Cd) is a highly toxic and widespread soil pollutant, which negatively affects various aspects of plant growth and physiology. Here, the role of photosynthesis in response to Cd was investigated in the Cd-tolerant pea (Pisum sativum L.) mutant SGECd^t. The wild type SGE and the mutant SGECd^t were grown in a hydroponic solution supplemented with 1, 3, or 4 µM CdCl₂ for 12 d. Root and shoot biomasses of the Cd-treated SGECd^t were significantly higher than of SGE. Cadmium had little effect on the quantum yield of photosystem II (φPSII) and chlorophyll content of intact leaves of both...

Quantitative trait loci for winter hardiness and other agronomic traits in the progeny of two amphiploid Festulolium hybrids (Festuca pratensis × Lolium perenne) backcrossed twice to tetraploid Lolium perenne Special Issue: Festulolium

K. TAMURA, Y. SANADA, K. TASE, Y. AKIYAMA, A. KUBOTA

Biologia plantarum 64: 439-446, 2020 | DOI: 10.32615/bp.2020.057

To clarify the effects of the introduction of Festuca pratensis-derived genomic regions in a tetraploid Lolium perenne genomic background, we analyzed quantitative trait loci (QTLs) for winter hardiness and eight other agronomic traits using two mapping populations generated by sequential backcrosses of two different amphiploid F. pratensis × L. perenne hybrids to tetraploid L. perenne. We constructed two interspecific linkage maps: FLBC2A (143 DNA markers, 301.2 cM total) and FLBC2C (126 markers, 256.2 cM), based on F. pratensis-derived alleles, with 72 markers in common. Interval mapping detected QTLs for...

Isolation and characterization of the promoter of SEPALLATA1-like gene from Platanus acerifoliaOriginal Papers

S.J. LU, S.S. YI, L. LIU, M.Z. BAO, G.F. LIU

Biologia plantarum 64: 430-438, 2020 | DOI: 10.32615/bp.2020.036

London plane (Platanus acerifolia Wild.) is a famous landscape plant because of its numerous desirable traits except the abundant pollens and seed hairs, which not only pollute the environment but also affect human health. To resolve these problems, we herein isolated and functionally analyzed the promoter of PlacSEP1.1, an orthologous gene of Arabidopsis SEPALLATA1, and investigated the potential usability for cell ablation strategies to engineer reproductive sterility in plants. A 2130 bp 5' upstream region of PlacSEP1.1 was isolated and termed pPlacSEP1.1. Putative motif detections show that there were several...

Effects of exogenous nitric oxide and ethylenediaminetetraacetic acid on cadmium toxicity and accumulation in ryegrassSpecial Issue: Gasotransmitters

Q. ZHANG, Y. Y. ZHU, Y. J. DONG

Biologia plantarum 64: 422-429, 2020 | DOI: 10.32615/bp.2020.046

The effects of exogenous nitric oxide (NO) and ethylenediaminetetraacetic acid (EDTA) on cadmium toxicity and accumulation in ryegrass (Lolium perenne L.) were studied in a hydroponic experiment. The results show that in plants without Cd application, addition of EDTA and sodium nitroprusside (SNP, an exogenous NO donor) significantly reduced the plant height, root length, and root activity of ryegrass, and significantly increased the O₂^*- generation rate and H₂O₂ and malondialdehyde (MDA) content in the aboveground and underground parts of ryegrass. Cadmium stress significantly inhibited ryegrass...

An HD-Zip IV transcription factor protein NbGL3 regulates glandular trichome initiation in tobaccoOriginal Papers

Y.S. TIAN, T.W. DAI, C.X. FAN, J. ZHOU, X.L. REN, Y. XU

Biologia plantarum 64: 378-384, 2020 | DOI: 10.32615/bp.2020.012

Glandular trichomes, a specialized multicellular structure, are considered as biofactories due to their capability to synthesize and secrete a large amount of secondary metabolites. Tobacco leaves have a high density of glandular trichomes that produce huge amounts of secondary metabolites, which can be used as important industrial raw materials. However, molecular mechanism controling glandular trichome development in tobacco still remains largely unknown. In the present study, we found that NbGL3, an HD-Zip IV family gene from Nicotiana benthamiana, was highly expressed in mature leaves, and ethylene or auxin application could increase...

Mannose regulates water balance, leaf senescence, and genes related to stress tolerance in white clover under osmotic stressOriginal Papers

S.Y. ZHAO, W.H. ZENG, Z. LI, Y. PENG

Biologia plantarum 64: 406-416, 2020 | DOI: 10.32615/bp.2020.017

Mannose (MAN), an important monosaccharide, contributes to coping with abiotic stresses in plants. Objectives of this study were to examine whether exogenous MAN (30 mM) could significantly increase drought tolerance and further to reveal MAN-regulated tolerance mechanism in white clover under osmotic stress induced by 18 % (m/v) polyethylene glycol 6000 for 10 d in controlled growth chambers. Results show that the application of MAN significanlty alleviated stress damage and the inhibition of growth and photosynthesis in white clover under osmotic stress. The MAN-induced increase in endogenous MAN content and the accumulation of organic osmolytes...

Reserve metabolism and ubiquitin proteasome system are involved in germination progress of wheat grainsOriginal Papers

W.D. ZHANG, L. WANG, Q.R. GAO, Y. WANG, Y.Z. WANG

Biologia plantarum 64: 395-405, 2020 | DOI: 10.32615/bp.2020.003

To further elucidate the molecular mechanism of the germination in common wheat, a differential proteomic analysis was completed between the F₁ hybrid RS5322 and its parental lines (RS53 and SN22) using isobaric tags for relative and absolute quantitation (iTRAQ) based strategy. Rapid grain germination and superior young seedling growth were observed in the F₁ hybrid line. A total of 807 differentially abundant proteins (DAPs) were identified by iTRAQ analysis of grains at 48 h after imbibition in distilled water. Bioinformatics analysis shows that 638 DAPs were annotated in 38 Gene Ontology functional groups, 764 DAPs...

A new approach to prevent hazelnut callus browning by modification of sub-cultureBrief Communications

M. RAEISPOUR SHIRAZI, S.A. RAHPEYMA, J. ZOLALA

Biologia plantarum 64: 417-421, 2020 | DOI: 10.32615/bp.2020.009

Tissue culture of hazelnut (Corylus avellana L.) represented the promising strategy for production of its valuable compound, paclitaxel, but one of the most important problems is initial callus browning in its callus culture. To obtain healthy callus cells of C. avellana, three different culture media, Murashige and Skoog (MS), Nas and Read (NRM), and Driver and Koniuki (DKW), in combination with 500 mg dm^-3 citric acid, 500 mg dm^-3 acetic acid, and 500 mg dm^-3 polyvinylpyrrolidone (PVP) were applied. These cultures were passed two sub-cultures in the same media. Also, a novel modified sub-culture system...

Identification and expression pattern analysis of YUCCA and ARF gene families during somatic embryogenesis of Lilium spp.Original Papers

M.-M. CHEN, X. LI, Y.-M. CAI, Y.-C. ZHANG, J.-J. GU, L.-Y. YANG

Biologia plantarum 64: 385-394, 2020 | DOI: 10.32615/bp.2020.026

Auxin is a key phytohormone in plant somatic embryogenesis (SE) and YUCCA and AUXIN RESPONSE FACTORS (ARFs) are two key genes involved in auxin biosynthesis and auxin signaling pathways, respectively. They have been reported in participating to the catalytic production of endogenous indole-3-acetic acid (IAA; a natural auxin) and regulating the transcription of auxin-responsive genes. To explore the structural characteristics of the YUCCA and ARF families of Lilium spp. L. and its expression pattern during SE induction and development processes, 6 YUCCA genes and 12 ARF genes were screened from...

Impacts of silicon and silicon nanoparticles on leaf ultrastructure and TaPIP1 and TaNIP2 gene expressions in heat stressed wheat seedlingsOriginal Papers

A.A. YOUNIS, H. KHATTAB, M.M. EMAM

Biologia plantarum 64: 343-352, 2020 | DOI: 10.32615/bp.2020.030

Heat stress is one of the most crucial factors affecting crop growth and productivity worldwide. So, searching for a potent eco-friendly heat stress alleviator is the main issue nowadays. The current study was conducted to assess the ameliorative effects of 1.5 mM potassium silicate (K₂SiO₃, further only Si) or 1.66 mM silicon dioxide nanoparticles (SiNPs) on wheat (Triticum aestivum L.) seedlings exposed to heat stress (45 °C, 4 h). The observations show that Si or SiNPs treatments significantly restored the heat stress-provoked ultrastructural distortions of cellular organelles, particularly chloroplasts and the nucleus....

Cellulose biosynthesis in plants - the concerted action of CESA and non-CESA proteinsReviews

M. JURANIEC, B. GAJDA

Biologia plantarum 64: 363-377, 2020 | DOI: 10.32615/bp.2020.065

Cellulose is the most abundant polysaccharide produced by plants. In the form of rigid microfibrils surrounding the cells, cellulose constitutes the load-bearing cell wall element that controls cell growth and shape. Cellulose microfibrils are laid down outside the cell by the multimeric plasma membrane-inserted cellulose synthase complexes (CSCs), which move along underlying cortical microtubules (CMTs). In plants, CSCs are shaped as rosettes with six lobes symmetrically arranged in a hexagonal structure. In Arabidopsis, the CSC is composed of at least three functionally non-redundant cellulose synthase (CESA) glycosyltransferases in both primary...

Foliar applications of spermidine improve foxtail millet seedling characteristics under salt stressOriginal Papers

M. SUN, T. WANG, L. FAN, H. WANG, H. PAN, X. CUI, Y. LOU, Y. ZHUGE

Biologia plantarum 64: 353-362, 2020 | DOI: 10.32615/bp.2019.158

This study investigated the mitigating effects of spermidine (Spd) application on salinity-induced ion inbalance, physiological properties, and the expression of some genes in foxtail millet (Setaria italica L.). We observed 30-d-old seedlings maintained at a half-strength Hoagland solution (control), 1.0 % NaCl, 10, 20, and 40 μM Spd, and 10, 20, and 40 μM Spd + 1.0 % (m/v) NaCl for 14 d. The results show that salt stress significantly inhibited plant growth, and this was significantly ameliorated by Spd. The mass of the shoots and roots, content of chlorophyll a and chlorophyll b, root activity, and K⁺ content...

Imazamox detoxification and recovery of plants after application of imazamox to an imidazolinone resistant sunflower hybridOriginal Papers

D. BALABANOVA, T. REMANS, A. CUYPERS, J. VANGRONSVELD, A. VASSILEV

Biologia plantarum 64: 335-342, 2020 | DOI: 10.32615/bp.2019.150

Imidazolinone herbicides combined with imidazolinone resistant (IMI-R) crops provide a tool for solving the important problem of the occurrence of weeds during the early growth stages of sunflower. These herbicides inhibit the synthesis of branched chain amino acids by interrupting the key enzyme acetohydroxyacid synthase (AHAS). We studied the imazamox detoxification in an IMI-R sunflower hybrid together with plant growth and photosynthetic performance. Inhibition of photosynthesis and growth were observed as initial effects of imazamox application. A slight decrease in AHAS activity was also noticed. These effects disappeared within two weeks after...

The classification of tetraploid wheat by phylogenetic and cytogenetic analysesOriginal Papers

S.-Y. LI, L. XI, H.-J. LIU, W. ZHU, L.-L XU, Y. WANG, J. ZENG, X. FAN, L.-N. SHA, H.-Q. ZHANG, W.-L. QI, G.-Y. CHEN, Y.-H. ZHOU, H.-Y. KANG

Biologia plantarum 64: 324-334, 2020 | DOI: 10.32615/bp.2020.013

Tetraploid wheat (Triticum turgidum L.) is an important species within the genus Triticum and harbors many desirable agronomic traits. The classification, origin, and evolution of tetraploid wheat remain confused and controversial, resulting in useless germplasm resources. Two classification systems for tetraploid wheat are widely used: 1) tetraploid wheat comprises two species; 2) all forms of tetraploid wheat are classified as one species. The present study aimed to reassess the classification of tetraploid wheat using phylogenetic analysis of nuclear rDNA internal transcribed spacer region (ITS) sequence data, fluorescence...

Effect of virus inducible cis-element insertion on transcription properties of improved GWSF promoter in Arabidopsis thalianaBrief Communications

Z.C. HUANG, H. LI

Biologia plantarum 64: 320-323, 2020 | DOI: 10.32615/bp.2020.032

An ideal synthetic promoter can accurately regulate gene expression and the minimal cauliflower mosaic virus 35S promoter (GWSF) is an ideal synthetic pathogen-inducible promoter (SPIP) with several advantages. Three modified SPIPs, named as VGWSF, GWVSF, and GWSFV according to the arrangement of cis-elements, were optimized by inserting the dimer of a virus inducible cis-element (TTGGGAAGGAATTTCCTACT, V-box) upstream, midstream, or downstream the GWSF sequence. The three promoters were used to replace the cauliflower mosaic virus 35S promoter in the plasmid pBI121 in order to control the expression of the β-glucuronidase...

Cloning and functional characterization of the β-amyrin synthase genefrom Bupleurum chinenseOriginal Papers

J.-C. LI, C. WANG, W.-T. QI, C.-L. LIU

Biologia plantarum 64: 314-319, 2020 | DOI: 10.32615/bp.2020.008

Bupleurum chinense DC., a source of the traditional Chinese medicine Bupleuri Radix, is rich in triterpenoid saikosaponins with high pharmacological activities. The enzyme β-amyrin synthase (BAS), which converts the precursor 2,3-oxidosqualene to produce the triterpene skeleton, is crucial for the biosynthesis of triterpenoid saponins. In this study, we cloned the full-length sequence of the BAS gene from B. chinense, conducted a bioinformatics analysis, and expressed it in Saccharomyces cerevisiae to investigate its function. The cDNA of β-amyrin synthase (BcBAS, GenBank accession number: MN186093) cloned...

Silicon alleviates salt and drought stress of Glycyrrhiza uralensis plants by improving photosynthesis and water statusOriginal Papers

W.J. ZHANG, X.J. ZHANG, D.Y. LANG, M. LI, H. LIU, X.H. ZHANG

Biologia plantarum 64: 302-313, 2020 | DOI: 10.32615/bp.2019.136

Silicon has been widely reported to have a beneficial effect on improving plant tolerance to biotic and abiotic stresses. However, the mechanisms of Si in mediating responses to simultaneous salt and drought stresses are still poorly understood. Glycyrrhiza uralensis Fisch. is classified as a non-Si accumulator and suffered from salt and drought stresses. In this study, we investigated the long-term application of Si on Si content in G. uralensis roots, stems and leaves, leaf anatomy, ultrastructure, chlorophyll (Chl) content, gas exchange characteristics, relative water content, and growth of two-year-old plants under different salt...

Comparative analysis of nitrogen metabolism-related genes in two inbred maize lines with different low-nitrogen toleranceOriginal Papers

M. YAN, J. LUO, L. LIANG, L. CHEN, Y.Y. CAO, Y.H. CHEN, X.Y. ZHU, C.M. YU

Biologia plantarum 64: 289-301, 2020 | DOI: 10.32615/bp.2019.093

Maize is an important crop and its nitrogen use efficiency (NUE) has been an issue for plant scientists and breeders for many years. To meet the demand of genetic diversity in cultivating local elite maize cultivars, researchers need to isolate germplasms with a high NUE. In this study, 30 maize inbred lines were screened under hydroponic conditions, and three inbred lines that tolerate low nitrogen concentration were identified. A comparative analysis of genes involved in N uptake, transport, and assimilation between two lines with different tolerances revealed that the low-nitrogen-tolerant inbred line MBST exhibited higher expressions of nitrate...

Isolation and expression profiles of class III PRX gene family under drought stress in Camellia sinensisOriginal Papers

H.J. LI, H.B. WANG, Y. CHEN, Q.P. MA, Z. ZHAO, X.H. LI, X. CHEN

Biologia plantarum 64: 280-288, 2020 | DOI: 10.32615/bp.2019.120

The class III PRX family is a class of heme-containing oxidases and plays important roles in response to abiotic stress in plants. The responses to abiotic stresses could be regulated by phytohormones like abscisic acid (ABA) and methyl jasmonate (MeJA). In this research, 11 CsPRXs genes in tea plant (Camellia sinensis) were cloned and analyzed. Based on the similarity of the sequences, they were classified into 5 sub-groups. According to the results of reverse transcription PCR, CsPRX55 presented the highest expression in roots compared to stems and leaves of both tea cultivars LJ43 and Baiye 1. Besides, the expressions of CsPRX12...

Identification of novel hypoxia-responsive factors in deep-water rice conferring tolerance to flood during germinationOriginal Papers

D. KUMAR, P.K. DAS, B.K. SARMAH

Biologia plantarum 64: 244-252, 2020 | DOI: 10.32615/bp.2019.128

Flood in rice fields at the time of seed sowing and early seedling establishment causes extensive crop loss due to the inability of the seeds to tolerate and overcome submergence. In the present study, rice genotypes from Assam, India tolerant to hypoxia during germination were identified through systematic screening of deep-water rice genotype collection from flood-prone ecosystem of Brahmaputra river valley. The difference in tolerance to hypoxia during germination within the species provides scope for identification of novel hypoxia-responsive factors involved in tolerance as mechanism of tolerance may not be conserved across tolerant germplasms....

Salt tolerance of potato genetically engineered with the Atriplex canescens BADH geneOriginal Papers

A. ALI, Q. ALI, M.S. IQBAL, I.A. NASIR, X. WANG

Biologia plantarum 64: 271-279, 2020 | DOI: 10.32615/bp.2019.080

Potato (Solanum tuberosum L.) is among the top staple foods in the world, and salinity adversely affects its yield and quality. To improve salt tolerance in potato, the present study is focused on the Agrobacterium-mediated transformation of potato by the Atriplex canescens betaine aldehyde dehydrogenase (BADH) gene driven by single, double, and triple CaMV 35S promoters. The study led first to the detection of seven lines containing the BADH gene followed by the identification of T-DNA insertions via DNA hybridization and enzyme-linked immunosorbent assays. The salt tolerance was found to be promoter dependent,...

Overexpression of a stilbene synthase gene from Fallopia multiflora causes trans-piceid accumulation in transgenic ArabidopsisBrief Communications

S. SHENG, W. XIA, W. RUI, W. ZHAO, T. SHE, J. XIE, S. ZHAO, L. CHEN

Biologia plantarum 64: 266-270, 2020 | DOI: 10.32615/bp.2019.152

Fallopia multiflora, also known as Polygonum multiflorum, is a traditional Chinese medicine with stilbenes as the main characteristic components. We previously isolated the presumed stilbene synthase gene FmSTS1 from F. multiflora and inserted the FmSTS1 gene into the genome of Arabidopsis thaliana under the control of cauliflower mosaic virus 35S promoter to evaluate its biological functions. Southern blot and Northern blot analyses were performed to confirm the integration and expression of FmSTS1 in Arabidopsis. A new stilbene compound, which was identified as trans-piceid, was found to accumulate...

Effects of potassium chloride and nitric oxide on growth and physiological characteristics of winter wheat under salt stressSpecial Issue: Gasotransmitters

Y.J. DONG, Q. ZHANG, X.L. DAI, M.R. HE

Biologia plantarum 64: 258-265, 2020 | DOI: 10.32615/bp.2019.091

A hydroponic culture was conducted to evaluate the effects of KCl and sodium nitroprusside (SNP; a nitric oxide donor) in wheat seedlings under salt stress. Exposure to 100 mM NaCl for 7 d decreased biomass of wheat seedlings, root activity and H⁺-ATPase activity, significantly increased free proline content, reactive oxygen species (ROS) accumulation and lipid peroxidation, and suppressed the activity of superoxide dismutase (SOD). Moreover, NaCl stress significantly decreased the K⁺ and increased the Na⁺ content. Addition of KCl or SNP led to the increase in root activity and soluble protein content, stimulated the...

Transcriptome-based validation of proper reference genes for reverse trascription quantitative PCR analysis of Sinocalycanthus chinensisOriginal Papers

C. ZHANG, Q.-N. JIANG, Y.-G. WANG, J.-X. FU, B. DONG, L.-H. ZHOU, H.-B. ZHAO

Biologia plantarum 64: 253-257, 2020 | DOI: 10.32615/bp.2020.016

Reverse transcription quantitative PCR is a widely used method to detect gene expressions. To obtain accurate expression results, the selection of proper reference genes is important and necessary. However, related works concerning reference gene selection have not been carried on many plant species, especially endangered ones. The aim of the present study was to select dependable reference genes for expression normalization of an endangered plant species with medicinal and ornamental value: Sinocalycanthus chinensis (Calycanthaceae). Nine reference genes with stable expressions were chosen for further analysis according to transcriptomic sequencing...

Do soil and leaf silicon content affect leaf functional traits in Deschampsia caespitosa from different habitats?Original Papers

M. GRAŠIČ, T. SAKOVIČ, D. ABRAM, K. VOGEL-MIKUŠ, A. GABERŠČIK

Biologia plantarum 64: 234-243, 2020 | DOI: 10.32615/bp.2019.155

The purpose of this study was to show the extent of phenotypic plasticity of the grass Deschampsia caespitosa from four habitats with different soil properties by comparing selected leaf traits and content of silicon and other elements. Morphological, biochemical, and optical properties were examined in leaves, but content of silicon and other elements also in soil samples. Plant-available silicon in the soil was determined following extraction in CaCl₂. Bulk element analysis was conducted using X-ray fluorescence spectrometry. The habitats of D. caespitosa differed significantly according to soil structure, which resulted...

Induction of somatic embryogenesis and evaluation of genetic stability in regenerated plants of Magnolia dealbataOriginal Papers

A. CHÁVEZ-CORTAZAR, M. MATA-ROSAS, K. OYAMA, M.S. SAMAIN, M. QUESADA

Biologia plantarum 64: 224-233, 2020 | DOI: 10.32615/bp.2020.006

The utility of plant tissue culture for the mass propagation of trees is well known, but continuous in vitro multiplication of plant material may increase the possibility of somaclonal variation; therefore, it is essential to evaluate the genetic integrity of regenerants from species-specific in vitro protocols prior to mass production and implementation. The objectives of this study were: 1) to determine the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration over two cycles of secondary somatic embryogenesis in Magnolia dealbata; and 2) to verify the genetic stability of the regenerants obtained. The...

Comparative proteomics of phloem exudates reveals long-distance signals potentially involved in Litchi chinensis floweringBrief Communications

L.-J. HUANG, H.-B. CHEN, Z.-Q. HU, X.-Y. LU, H.-Y. WANG, H. LIU, B.-Y ZHOU

Biologia plantarum 64: 220-224, 2020 | DOI: 10.32615/bp.2019.163

It is well known that phloem is essential for transporting proteins as long-distance signals. In this study, a proteomic approach was carried out to identify proteins accumulated in phloem exudates at the pre-floral induction (PFId) stage, at the floral induction (FId) stage, and at the floral initiation (FIn) stage. As a result, 45 phloem exudate proteins were detected. Six proteins were found at all three stages. Sixteen proteins were specific to the PFId stage, 14 proteins were specific to the FId and FIn stages. From the enriched gene ontology (GO)-terms related to transport, signaling, hormone, and development, 12 transport-related, 5 signaling-related,...