Markéta Bocková, Ph.D.

Research interests:

Surface plasmon resonance (SPR) biosensors.

Functional coatings and assays for detection of biomolecules and investigation of biomolecular interactions.

Affinity biosensors for medical diagnostics. 

Education:

2010 - 2019: Doctoral Study: Charles University, Faculty of Mathematics and Physics, Department of Biophysics
Thesis: Multifunctional biomolecular systems for parallelized analysis of biomolecular interaction

2007 - 2009: Master Study: Charles University, Faculty of Science, Department of Biochemistry
Thesis: Study of biomolecular interactions by the method of surface plasmon resonance

2004 - 2007: Bachelor study: Charles University, Faculty of Science, Department of Biochemistry
Thesis: Optical biosensors and study of biomolecular interactions.

Publications:

Journal papers:

1. O. Pastva, L. Chrastinová, M. Bocková, R. Kotlín, J. Suttnar, A. Hlaváčková, J. Štikarová, E.  Ceznerová, J. Čermák, J. Homola, J. E. Dyr: Hsp70 trap assay for detection of misfolded subproteome related to Myelodysplastic syndromes, Analytical Chemistry, 91, 14226-14230 (2019).
2. M. Kalousová, S. Dusilová-Sulková, A. Kuběna, O. Zakiyanov, K Levová, M. Bocková, E. Gedeonová, X. Chadtová Song, M. L. Ermini, T. Špringer, J. Homola, V. Tesař, and T. Zima: Pregnancy-associated plasma protein A2 in hemodialysis patients: significance for prognosis, Kidney and Blood Pressure Research, 42, 509-518 (2017)
3. L. Chrastinová, O. Pastva, M. Bocková, N. S. Lynn, P. Šácha, M. Hubálek, J. Suttnar, R. Kotlín, J. Štikarová, A. Hlaváčková, K. Pimková, J. Čermák, J. Homola, and J. E. Dyr: A new approach for the diagnosis of myelodysplastic syndrome subtypes based on protein interaction analysis, Scientific Reports, 9, Article number 12647 (2019).
4. M. Bocková, J. Slabý, T. Špringer, J. Homola: Advances in surface plasmon resonance imaging and microscopy and their biological applications, Annual Review of Analytical Chemistry, 12, 151–176 (2019).
5. B. Špačková, P. Wrobel, M. Bocková, J. Homola: Optical biosensors based on plasmonic nanostructures: a review, Proceedings of the IEEE, 104, 2380–2408 (2016).
6. C. Bessa-Pereira, M. Bocková, R. F. Santos, A. M. Santos, M. Martins de Araújo, L. Oliveira, J. Homola, A. M. Carmo: The scavenger receptor SSc5D physically interacts with bacteria through the SRCR-containing N-terminal domain, Frontiers in Immunology, 7, Article 416, 1-9 (2016).
7. M. Bocková, X. Xue Chadtová Song, E. Gedeonová, K. Levová, M. Kalousová, T. Zima, J. Homola: Surface plasmon resonance biosensor for detection of pregnancy associated plasma protein A2 in clinical samples, Analytical and Bioanalytical Chemistry, 408, 7265–7269.
8. N. S. Lynn, M. Bocková, P. Adam, J. Homola: Biosensor enhancement using grooved micromixers: part II, experimental studies, Analytical Chemistry, 87, 5524-5530 (2015).
9. M. Bocková, T. Špringer, I. Nečasová, J. Nunvar, B. Schneider, and J. Homola: Monitoring RAYT activity by surface plasmon resonance biosensor, Analytical and Bioanalytical Chemistry, 407, 3985-3993 (2015).
10. H. Vaisocherová, I. Víšová, M. Bocková, T. Špringer, M. L. Ermini, X. Song, Z. Krejčík, L. Chrastinová, O. Pastva, K. Pimková, M. Dostálová Merkerová, J. E. Dyr, and J. Homola: Rapid and sensitive detection of multiple MicroRNAs in cell lysate by low-fouling surface plasmon resonance biosensor, Biosensors and Bioelectronics, 70, 226–231 (2015).
11. N. S. Lynn Jr., J. Martínez-López, M. Bocková, P. Adam, V. Coello, H. R. Siller, J. Homola: Biosensing enhancement using passive mixing structures for microarray-based sensors, Biosensors and Bioelectronics, 54 (2014) 506-514.
12. T. Špringer, M. Bocková, J. Homola: Label-Free Biosensing in Complex Media: A Referencing Approach, Analytical Chemistry, 85 (2013) 5637–5640.
13. Z. Krištofiková, D. Řípová, A. Bartoš, M. Bocková, K. Hegnerová, J. Říčný, L. Čechová, M. Vrajová, J. Homola: Neuroinflammation and Complexes of 17ß-Hydroxysteroid Dehydrogenase type 10 – Amyloid ß in Alzheimer´s Disease, Current Alzheimer Research, 10 (2013) 165-173.
14. M. Vala, R. Robelek, M. Bocková, J. Wegener, J. Homola: Real-time label-free monitoring of the cellular response to osmotic stress using conventional and long-range surface plasmons, Biosensors and Bioelectronics, Biosensors and Bioelectronics 40 (2013) 417–421.
15. P. Kvasnička, K. Chadt, M. Vala, M. Bocková, and J. Homola: Toward single-molecule detection with sensors based on propagating surface plasmons, Optics letters, 37 (2012) 163 – 165. 
16. K. Pimková, M. Bocková, K. Hegnerová, J. Suttnar, J. Čermák, J. Homola, J. E. Dyr: Surface plasmon resonance biosensor for the detection of VEGFR-1—a protein marker of myelodysplastic          syndromes, Analytical and Bioanalytical Chemistry, 402 (2012) 381–387.
17. H. Šípová, M. Piliarik, M. Vala, K. Chadt, P. Adam, M. Bocková, K. Hegnerová, J. Homola: Portable Surface Plasmon Resonance Biosensor for Detection of Nucleic Acids, Procedia Engineering, 25 (2011) 148-151. 
18. M. Piliarik, M. Bocková, J. Homola: Surface plasmon resonance biosensor for parallelized detection of protein biomarkers in diluted blood plasma,  Biosensors and Bioelectronics, 26 (2010) 1656-1661.
19. S. Herranz, M. Bocková, M. D. Marazuela, J. Homola, M. C. Moreno-Bondi: An SPR biosensor for the detection of microcystins in drinking water, Analytical and Bioanalytical Chemistry 398 (2010) 2625–2634. 
20. Z. Krištofiková, M. Bocková, K. Hegnerová, A. Bartoš, J. Klaschka, J. Říčný, D. Řípová, J. Homola: Enhanced levels of mitochondrial enzyme 17ß-hydroxysteroid dehydrogenase type 10 in patients with Alzheimer disease and multiple sclerosis, Molecular Biosystems 5, (2009) 1174-1179.
21. K. Hegnerová, M. Bocková, H. Vaisocherová, Z. Krištofiková, J. Říčný, D. Řípová, J. Homola: Surface plasmon resonance biosensors for detection of Alzheimer disease biomarkers, Sensors and Actuators B-Chemical. 139, (2009) 69-73.
22. H. Vaisocherová, A. Zítová, M. Lachmanová, J. Štěpánek, Š. Králíková, R. Liboska, D. Rejman, I. Rosenberg, and J. Homola, Investigating oligonucleotide interactions at subnanomolar level by surface plasmon resonance biosensor, Biopolymers, 82 (2006), 394 – 398.

Conference papers:

1. Bockova M., Chadtova Song X., Gedeonova E., Levova K., Kalousova M., Zima T., Homola J.: Detection of Pregnancy associated plasma protein A2 in clinical samples, Biosensors 2016 - 26th Anniversary World Congress on Biosensors, Gothenburg, 2016.
2. M. Bocková, N. S. Lynn Jr., T. Špringer, J. Homola:  SPR imaging-based microarray for enhanced multianalyte detection in blood plasma, Biodetection & Biosensors, Berlin, 2015. Oral presentation
3. J. E. Dyr, L. Chrastinova, M. Bockova, H. Vaisocherova, O. Pastva, J. Suttnar, R. Kotlin, J. Homola: Blood Plasma of Patients with Myelodysplastic Syndromes analysed by Surface Plasmon Resonance Imaging and Mass Spectrometry, 3rd International Conference on BioPhotonics Florence, 2015.
4. J. E. Dyr, J. Cermak, H. Vaisocherova, Z. Krejcik, H. Sipova, M. Bockova, I. Visova, T. Springer, M.L. Ermini, L. Chrastinova, O. Pastva, K. Pimkova, R. Kotlin, J. Novak, J. Homola: Rapid Simultaneous Detection of Multiple Micro-Ribonucleic Acids By Surface Plasmon Resonance Array in Cell Lysates of Myelodysplastic Syndromes Patients, 56th ASH Annual Meeting & Exposition, San Francisco, 2014.
5. O. Pastva, K. Pimková, L. Chrastinová, M. Bocková, J. Suttnar, R. Kotlín, J. Novák, J. Čermák, J. Homola, J. E. Dyr: Protein-Protein Interaction Analysis in Blood Plasma of Patients with Myelodysplastic Syndromes by Surface Plasmon Resonance Imaging and Mass Spectrometry, 56th ASH Annual Meeting & Exposition, San Francisco, 2014.
6. M. Bocková, N. S. Lynn Jr., J. Martínez-López, J. Homola: Passive mixing structures for sensing performance enhancement of high-throughput sensors, EUROPT(R)ODE XII, Athens, 2014.
7. N. S. Lynn, M. Bockova, J. Slaby, J. Homola: Enhancement of affinity biosensors using the staggered herringbone mixer, EUROPT(R)ODE XII, Athens, 2014. Oral presentation
8. M. Bocková, J. Homola: Investigating protein - protein interactions by surface plasmon resonance based biosensor, Spetsai Summer School 2013 - Protein interactions, assemblies and human disease, Spetses (Greece), 2013.
9. K. Pimková, M. Bocková, J. Suttnar, P. Májek, R. Kotlín, J. Homola, J.E. Dyr: Searching for protein team players in MDS, 3rd International Conference on Bio-Sensing Technology, Sitges (Spain) 2013.
10. M. Bocková, K. Pimková, J. Homola: Blood plasma depletion for the sensitive detection of cancer biomarkers using an SPR biosensor, Label-free technologies: advances and applications, Amsterdam, 2012.
11. M. Bocková, J. Homola: Poly-L-lysine based surface chemistries for protein and cell immobilization, XXIII. biochemický sjezd, Brno, 2012.
12. M. Bocková, K. Pimková, V. Ševců, H. Šípová, J. E. Dyr a J. Homola: Protein chip for diagnosis of myelodysplastic syndromes using SPR imaging, Biosensors 2012 – 22nd Anniversary World Congress on Biosensors, Cancun, 2012. 
13. M. Vala, R. Robelek, M. Bocková, J. Wegener, J. Homola: Monitoring the cellular response to osmotic stress using conventional and long-range surface plasmons, Biosensors 2012 – 22nd Anniversary World Congress on Biosensors, Cancun, 2012. 
14. H. Šípová, M. Bocková, J. Štěpánek, J. Homola: Hybridization on DNA chips: Influence of cations and probe density on hybridization dynamics, Biosensors 2012 – 22nd Anniversary World Congress on Biosensors, Cancun, 2012. 
15. K. Pimková, M. Bocková, V. Ševců, J. Suttnar, J. Homola, J. E. Dyr: Detection of T-lymphocyte activation antigen CD86 – a protein marker of myelodysplastic syndromes – using a surface plasmon resonance, EUROPT(R)ODE XI, Barcelona, 2012.
16. K. Pimková, J. Suttnar, M. Bocková, R. Kotlín, P. Májek, J. Homola, J. E. Dyr: Plasma sample pre-treatment for SPR protein chip for MDS diagnosis, EMBO Conference Series, Cancer Proteomics, Dublin, 2011. 
17. Z. Krištofiková, D. Řípová, M. Bocková, K. Hegnerová, J. Říčný, A. Bartoš, L. Čechová, J. Homola: New biomarkers of Alzheimer disease: Complexes of amyloid beta peptides and of different endogenous substances in cerebrospinal fluid, 10th World Congress of Biological Psychiastry, Prague, 2011.
18. K. Pimková, M. Bocková, R. Kotlín, J. Suttnar, J. Homola, J. E. Dyr: SPR chip for MDS diagnosis based on protein-protein interaction, The 11th International Symposium On Myelodysplastic Syndromes, Edinburgh, 2011.
19. M. Bocková, K. Hegnerová, J. Homola: Comparison of surface chemistries for protein immobilization onto NH2-rich surfaces, SPIE Optics + Optoelectronics, Prague 2011.
20. M. Piliarik, M. Bocková, J. Homola: Advances in Surface Plasmon Resonance Imaging for parallelized Detection of Biomarkers, SPIE Photonics West 2011, San Francisco 2011.
21. S. Herranz, M. Bocková, J. Homola, M. D. Marazuela, M. C. Moreno-Bondi: SPR biosensor for the detection of microcystins in environmental samples, BIOSENSORS 2010 - 20th Anniversary World Congress on Biosensors, Glasgow 2010.
22. M. Piliarik, M. Bocková, J. Homola: Advancing high-throughput surface plasmon resonance sensors, EUROPT(R)ODE X, Prague 2010.
23. S. Herranz, M. Bocková, J. Homola, M. D. Marazuela, M. C. Moreno-Bondi: SPR biosensor for the detection of microcystins in environmental samples, EUROPT(R)ODE X, Prague 2010. 
24. J. Suttnar, M. Bocková, K. Pimková, P. Májek, R. Kotlín, J. Homola, J. E. Dyr: Human plasma depletion techniques for label-free detection of low-abundance plasma proteins, EUROPT(R)ODE X, Prague 2010.
25. Z. Kristofikova, K. Hegnerova, M. Bockova, A. Bartos, J. Ricny, D. Ripova and J. Homola: Complexes of amyloid beta peptides and of 17beta-hydroxysteroid dehydrogenase type 10 in cerebrospinal fluid, 34th FEBS congress, Prague 2009.
26. Z. Kristofikova, K. Hegnerova, M. Bockova, A. Bartos, J. Ricny, D. Ripova and J. Homola: Complexes of amyloid beta peptides and of 17beta-hydroxysteroid dehydrogenase type 10 in cerebrospinal fluid, FEBS Journal 276, (2009) 148.
27. K. Hegnerová, M. Bocková, Z. Krištofiková, J. Říčný, D. Řípová,  J. Homola: Optické biosenzory s povrchovými plazmony a biomarkery Alzheimerovy nemoci, XIV. Celostátní conference biologické psychiatrie s mezinárodní účastí, Luhačovice 2009.
28. Z. Krištofiková, K. Hegnerová, M. Bocková, A. Bartoš, J. Říčný, D. Řípová and J. Homola: Analysis of the complex between amyloid beta peptides and mitochondrial enzyme 17beta-HSD10 in cerebrospinal fluid, 33rd FEBS congress, Athens 2008.
29. D. Řípová, Z. Krištofiková, A.Bartoš, J. Říčný, K. Hegnerová, M. Bocková and J. Homola: Could be levels of mitochondrial enzyme 17-HSD10 in cerebrospinal fluid a new biomarker of Alzheimer disease?, 18th Alzheimer Europe Conference, Oslo 2008.
30. K. Hegnerová, M. Bocková, H. Vaisocherová, Z. Krištofiková, J. Říčný, D. Řípová, J. Homola: Surface plasmon resonance biosensors for detection of Alzheimer disease biomarkers, Europtrode IX, Dublin, 2008
31. Krištofiková Z, Řípová D, Hovorková P, Hořínek A, Majer E, Homola J, Vaisocherová H, Bocková M, and Hegnerová K: Amyloid beta – binding protein 17beta-hydroxysteroiddehydrogenase type 10 and Alzheimer disease, III Meeting on the Molecular Mechanism of Neurodegeneration, Milano, Italy 2007 
32. H. Vaisocherová, A. Zítová, M. Lachmanová, P. Tobiška, and J. Homola, Monitoring oligonucleotide hybridization at subnanomolar level by surface plasmon resonance biosensor: Optimization of surface chemistry, In 9th World Congress on Biosensors - Book of Abstracts, Toronto, Canada, 2006, 400. 
33. H. Vaisocherová, A. Zítová, M. Lachmanová, M. Piliarik, J. Štěpánek, and J. Homola: Investigating oligonucleotide interactions by SPR biosensor, Gordon Research Conference on Chemical Sensors and Interfacial Design, Oxford, United Kingdom 2005.