Photosynthetica, 2020 (vol. 58), SPECIAL ISSUE

Photosynthetica 2020, 58(SI):236-244 | DOI: 10.32615/ps.2019.142

Special issue in honour of Prof. Reto J. Strasser – Photosynthesis response of microalgae (Tetradesmus wisconsinensis) to different inorganic carbon sources probed with chlorophyll fluorescence analysis

E. JANKA1, I. UMETANI2, M. SPOSOB1, R. BAKKE1
1 Department of Process, Energy and Environmental Technology, University of South-Eastern Norway, 3901 Porsgrunn, Norway
2 Department of Natural Sciences and Environmental Health, University of South-Eastern Norway, 3800 Bø, Norway

Bicarbonate is an alternative carbon source for production of photosynthetic microalgae, although sparging of CO2-enriched gas is commonly practised, its efficiency seems to be scarcely considered. This study evaluated the photosynthetic activities of microalgae (Tetradesmus wisconsinensis) using chlorophyll fluorescence under different concentrations of bicarbonate (HCO3-) and CO2. Chlorophyll fluorescence analysis showed noticeable difference in photosynthetic activity between the bicarbonate (HCO3-) and CO2 supplied cultures. The JIP-test parameters obtained for the cultures supplemented by HCO3- or CO2 were distinct, but of similar values in quantum yield of PSII. The typical polyphasic rise, called the OJIP curves, showed a typical difference between the treatments at the J and I inflection of the fluorescence transient curve. Both HCO3- (20 mM) and CO2 (5%, v/v) gave higher biomass yield than the other carbon regimes, i.e., 673 ± 12 and 658 ± 11 mg L-1, respectively. We concluded that bicarbonate resulted in similar photosynthetic yield as CO2 supplementation.

Additional key words: growth; medium; optical density; principal component analysis.

Received: August 31, 2019; Accepted: October 22, 2019; Prepublished online: November 26, 2019; Published: May 28, 2020Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
JANKA, E., UMETANI, I., SPOSOB, M., & BAKKE, R. (2020). Special issue in honour of Prof. Reto J. Strasser – Photosynthesis response of microalgae (Tetradesmus wisconsinensis) to different inorganic carbon sources probed with chlorophyll fluorescence analysis. Photosynthetica, 58(SPECIAL ISSUE), 236-244. doi: 10.32615/ps.2019.142.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abinandan S., Shanthakumar S.: Evaluation of photosynthetic efficacy and CO2 removal of microalgae grown in an enriched bicarbonate medium. - 3 Biotech. 6: 9, 2016. Go to original source...
    2. Baker N.R.: Chlorophyll fluorescence: a probe of photosynthesis in vivo. - Annu. Rev. Plant Biol. 59: 89-113, 2008. Go to original source...
    3. Baker N.R., Rosenqvist E.: Applications of chlorophyll fluorescence can improve crop production strategies: an examination of future possibilities. - J. Exp. Bot. 55: 1607-1621, 2004. Go to original source...
    4. Chen Y., Zhang L., Xu C., Vaidyanathan S.: Dissolved inorganic carbon speciation in aquatic environments and its application to monitor algal carbon uptake. - Sci. Total Environ. 541: 1282-1295, 2016. Go to original source...
    5. Ducat D.C., Way J C., Silver P.A.: Engineering cyanobacteria to generate high-value products. Trends Biotechnol. - 29: 95-103, 2011. Go to original source...
    6. Gardner R.D., Lohman E., Gerlach R. et al.: Comparison of CO2 and bicarbonate as inorganic carbon sources for triacylglycerol and starch accumulation in Chlamydomonas reinhardtii. - Biotechnol. Bioeng. 110: 87-96, 2013. Go to original source...
    7. Genty B., Briantais J.M., Baker N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - BBA-Gen. Subjects 990: 87-92, 1989. Go to original source...
    8. Govindjee: Chlorophyll a fluorescence: A bit of basics and history. - In: Papageorgiou G.C., Govindjee (ed.): Chlorophyll a Fluorescence: A Signature of Photosynthesis. Pp. 1-41. Springer, Dordrecht 2004. Go to original source...
    9. Heng R.L., Pilon L.: Time-dependent radiation characteristics of Nannochloropsis oculata during batch culture. - J. Quant. Spectrosc. Ra. - 144: 154-163, 2014. Go to original source...
    10. Kalaji H.M., Govindjee, Bosa K. et al.: Effects of salt stress on photosystem II efficiency and CO2 assimilation of two Syrian barley landraces. - Environ. Exp. Bot. 73: 64-72, 2011. Go to original source...
    11. Kalaji H.M., Oukarroum A., Alexandrov V. et al.: Identification of nutrient deficiency in maize and tomato plants by in vivo chlorophyll a fluorescence measurements. - Plant Physiol. Bioch. 81: 16-25, 2014. Go to original source...
    12. Kim H.W., Vannela R., Zhou C. et al.: Photoautotrophic nutrient utilization and limitation during semi-continuous growth of Synechocystis sp. PCC6803. - Biotechnol. Bioeng. 106: 553-563, 2010. Go to original source...
    13. Lazár D.: The polyphasic chlorophyll a fluorescence rise measured under high intensity of exciting light. - Funct. Plant Biol. 33: 9-30, 2006. Go to original source...
    14. Lee E., Jalalizadeh M., Zhang Q.: Growth kinetic models for microalgae cultivation: A review. - Algal Res. 12: 497-512, 2015. Go to original source...
    15. Malapascua J.R.F., Jerez C.G., Sergejevová M. et al.: Photo-synthesis monitoring to optimize growth of microalgal mass cultures: application of chlorophyll fluorescence techniques. -Aquat. Biol. 22: 123-140, 2014. Go to original source...
    16. Masojídek J., Torzillo G., Koblížek M.: Photosynthesis in microalgae. - In: Richmond A., Hu Q. (ed.): Handbook of Microalgal Culture. Pp. 21-36. John Wiley & Sons, Ltd., Chichester 2013. Go to original source...
    17. Maxwell K., Johnson G.N.: Chlorophyll fluorescence - a practical guide. - J. Exp. Bot. 51: 659-668, 2000. Go to original source...
    18. Meier L., Pérez R., Azócar L. et al.: Photosynthetic CO2 uptake by microalgae: An attractive tool for biogas upgrading. - Biomass Bioenerg. 73: 102-109, 2015. Go to original source...
    19. Mokashi K., Shetty V., George S.A., Sibi G.: Sodium bicarbonate as inorganic carbon source for higher biomass and lipid production integrated carbon capture in Chlorella vulgaris. - Achiev. Life Sci. - 10: 111-117, 2016. Go to original source...
    20. Murchie E.H., Lawson T.: Chlorophyll fluorescence analysis: a guide to good practice and understanding some new applications. - J. Exp. Bot. 64: 3983-3998, 2013. Go to original source...
    21. Nguyen B.T., Rittmann B.E.: Effects of inorganic carbon and pH on growth kinetics of Synechocystis sp. PCC 6803. - Algal Res. 19: 363-369, 2016. Go to original source...
    22. Oh-Hama T., Miyachi S.: Chlorella. - In: Borowitzka M.A., Borowitzka L.J. (ed.): Micro-Algal Biotechnology. Pp. 3-26. Cambridge Univeristy Press, Cambridge 1998.
    23. Oukarroum A.: Change in photosystem II photochemistry during algal growth phases of Chlorella vulgaris and Scenedesmus obliquus. - Curr. Microbiol. 72: 692-629, 2016. Go to original source...
    24. Sayre R.: Microalgae: The potential for carbon capture. - BioScience 60: 722-727, 2010. Go to original source...
    25. Stirbet A., Govindjee: On the relation between the Kautsky effect (chlorophyll a fluorescence induction) and Photosystem II: Basics and applications of the OJIP fluorescence transient. - J. Photoch. Photobio. B 104: 236-257, 2011. Go to original source...
    26. Strasser R.J., Srivastava A.: Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria. - Photochem. Photobiol. 61: 32-42, 1995. Go to original source...
    27. Strasser R.J., Srivastava A., Tsimilli-Michael M.: The fluores- cence transient as a tool to characterize and screen photosyn-thetic samples. - In: Yunus M., Pathre U., Mohanty P. (ed.): Probing Photosynthesis: Mechanism, Regulation and Adapta-tion. Pp. 445-483. CRC Press, New York 2000.
    28. Wang Y., Ho S.H., Cheng C.L. et al.: Perspectives on the feasibility of using microalgae for industrial wastewater treatment. - Bioresource Technol. 222: 485-497, 2016. Go to original source...
    29. Zhao J.M., Ma C.Y., Liu L.H.: Temporal scaling of the growth dependent optical properties of microalgae. - J. Quant. Spectrosc. Ra. 214: 61-70, 2018. Go to original source...

    Return to the content