Photosynthetica, 2015 (vol. 53), issue 2

Photosynthetica 2015, 53(2):269-278 | DOI: 10.1007/s11099-015-0104-7

Photosynthetic activities, C3 and C4 indicative enzymes and the role of photoperiod in dormancy induction in 'Chunjie' peach

H. S. Zhang1, D. M. Li2, Q. P. Tan2, H. Y. Gao1, D. S. Gao2,*
1 College of Life Sciences, Postdoctoral Station of Biology, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China
2 College of Horticulture Science and Engineering, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China

Our study examined the relationship between photosynthetic performance and activities of key photosynthetic enzymes to understand the photosynthetic variation and reasons for the variation during dormancy induction under different photoperiods in peach (Prunus persica L. cv. Chunjie). Furthermore, the study explained the changes in the key enzymes from the viewpoint of differential proteomics. The results showed that the leaf net photosynthetic rate (P N) and stomatal conductance tended to decrease, while the intercellular CO2 concentration rose, which indicated that the reduced P N resulted from nonstomatal limitation. During the dormancy induction period, the activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and phosphoenolpyruvate carboxylase (PEPC) declined, which was the main reason for the reduced P N. Two-dimensional electrophoresis maps and differential protein identification demonstrated that the decrease in activity of the photosynthetic enzymes was mainly due to enzymatic degradation. The enzyme degradation by a long-day treatment occurred later and to a lesser degree than that of the short-day treatment. In the long-day treatment, the carboxylation activity of Rubisco was higher than that of the control treatment, and the PEPC activity and the ratio of the PEPC/Rubisco activity were lower than the corresponding activities during the control treatment. These differences under long-day conditions were significant but did not occur in the short-day treatment, suggesting that the C4 pathway might be more active under short-day conditions.

Keywords: gas exchange; MALDI-TOF; nonstomatal limitation; Rubisco large subunit; two-dimensional electrophoresis

Received: November 25, 2013; Accepted: May 2, 2014; Published: June 1, 2015Show citation

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Zhang, H.S., Li, D.M., Tan, Q.P., Gao, H.Y., & Gao, D.S. (2015). Photosynthetic activities, C3 and C4 indicative enzymes and the role of photoperiod in dormancy induction in 'Chunjie' peach. Photosynthetica, 53(2), 269-278. doi: 10.1007/s11099-015-0104-7.
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