Photosynthetica, 2020 (vol. 58), SPECIAL ISSUE

Photosynthetica 2020, 58(2):358-368 | DOI: 10.32615/ps.2019.172

Special issue in honour of Prof. Reto J. Strasser – Action of alamethicin in photosystem II probed by the fast chlorophyll fluorescence rise kinetics and the JIP-test

W. XIAO1, H. WANG1, W. LIU1, X. WANG1, Y. GUO1, R.J. STRASSER1,2, S. QIANG1, S. CHEN1, Z. HU3
1 Weed Research Laboratory, Nanjing Agricultural University, 210095 Nanjing, China
2 Bioenergetics Laboratory, University of Geneva, CH-1254 Jussy/Geneva, Switzerland
3 Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 475004 Kaifeng, China

Alamethicin (AMT) is a linear antimicrobial peptide isolated from fungi Trichoderma viride. To date, the mode of action of AMT in plant cells remains unknown. Our experimental results indicate that AMT causes leaf lesion attributed to its multiple effects on PSII. AMT decreases the O2 evolution rate of PSII. Based on chlorophyll fluorescence data, similar to the classical herbicide diuron, AMT interrupts PSII electron transfer beyond QA at the acceptor side, leading to the inactivation of the PSII reaction centers. Additionally, AMT decreases chlorophyll content and destroys the architecture of PSII pigment assemblies. However, AMT does not affect the oxygen-evolving complex at the donor side of PSII. Thus, it is concluded that AMT is a natural photosynthetic inhibitor with several action sites in PSII.

Keywords: bioherbicide; chlorophyll a fluorescence; leaf lesion; natural product.

Received: August 28, 2019; Revised: November 27, 2019; Accepted: December 13, 2019; Prepublished online: January 17, 2020; Published: April 7, 2020Show citation

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XIAO, W., WANG, H., LIU, W., WANG, X., GUO, Y., STRASSER, R.J., ... HU, Z. (2020). Special issue in honour of Prof. Reto J. Strasser – Action of alamethicin in photosystem II probed by the fast chlorophyll fluorescence rise kinetics and the JIP-test. Photosynthetica, 58(SPECIAL ISSUE), 358-368. doi: 10.32615/ps.2019.172.
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