Photosynthetica, 2019 (vol. 57), issue 2

Photosynthetica 2019, 57(2):617-626 | DOI: 10.32615/ps.2019.056

Photoinhibition and continuous growth of the wild-type and a high-light tolerant strain of Chlamydomonas reinhardtii

O. VIRTANEN1, D. VALEV1, O. KRUSE2, L. WOBBE2, E. TYYSTJÄRVI1
1 University of Turku, Department of Biochemistry/Molecular Plant Biology, 20014 Turku, Finland
2 Bielefeld University, Algae Biotechnology and Bioenergy, Faculty of Biology, Center for Biotechnology (CeBiTec), Universitätsstrasse 27, 33615 Bielefeld, Germany

The volumetric productivity of the high-light tolerant strain hit2 of Chlamydomonas reinhardtii was found to be higher than that of the parental strain CC124 during continuous growth at PPFD from 200 to 1,500 µmol m-2 s-1. At PPFD of 1,250 µmol m-2 s-1, hit2 produced 2.53 ± 0.18 and CC124 produced 2.05 ± 0.12 g(biomass) dm-3 d-1. The rate constant of photoinhibition of hit2 was less than half of that of CC124, suggesting that hit2 produces more biomass than CC124 because hit2 does not need to allocate as much resources for PSII repair as CC124. Growth in high light triggered similar loss of chlorophyll, increase in the carotenoid-to-chlorophyll ratio, and decrease in PSI fluorescence in both strains. Thermoluminescence B band was shifted toward the Q band in hit2, suggesting that low redox potential of the QB/QB- pair contributes to the photoinhibition tolerance of hit2.

Additional key words: algae; biomass production; carotenoid; growth rate; nonphotochemical quenching; turbidostat.

Received: September 22, 2018; Accepted: March 15, 2019; Prepublished online: April 29, 2019; Published: May 16, 2019Show citation

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VIRTANEN, O., VALEV, D., KRUSE, O., WOBBE, L., & TYYSTJÄRVI, E. (2019). Photoinhibition and continuous growth of the wild-type and a high-light tolerant strain of Chlamydomonas reinhardtii. Photosynthetica, 57(2), 617-626. doi: 10.32615/ps.2019.056.
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