Biologia plantarum 64: 642-652, 2020 | DOI: 10.32615/bp.2019.162

Cadmium and zinc uptake and translocation in dwarf Polish wheat seedlings as affected by calcium and potassium combination

W. SHUAI1, Q. YAO1, X. CHEN1, Y. CHENG1, X. XIAO1, J. ZENG2,*, X. FAN1,3, H. KANG1 3, L. SHA1,3, H. ZHANG1,3, Y. ZHOU1,3, Y. WANG1,3,*
1 Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, P.R. China
2 College of Resources, Sichuan Agricultural University, Wenjiang 611130, Sichuan, P.R. China
3 Joint International Research Laboratory of Crop Resources and Genetic Improvement, Sichuan Agricultural University, Wenjiang 611130, Sichuan, P.R. China

Combination of calcium and potassium (Ca-K) influences cadmium and zinc uptake and translocation in dwarf Polish wheat (Triticum polonicum), but its effects remain unclear. In the present study, a high concentration of Ca-K reduced uptake of Cd and Zn by roots and promoted their translocations to shoots under Cd and Zn excess. Whatever under a low or high concentration of Ca-K, Zn inhibited Cd uptake and translocation under Cd+Zn stress when compared with Cd stress alone. However, the reduced Cd content caused by Zn under the high concentration of Ca-K was significantly lower than under the low concentration of Ca-K. Under both Ca-K treatments, Cd promoted Zn uptake and inhibited Zn translocation under Cd+Zn stress when compared with those under Zn stress. The high concentration of Ca-K reinforced the promotion of Zn uptake and the inhibition of Zn translocation caused by Cd. The Ca-K or Zn affected the expression of several metal transporters and influenced cell wall metabolism, the subcellular distribution of Cd, and the Cd chemical forms. Meanwhile, Ca-K or Cd also affected the expressions of several metal transporters and changed the subcellular distribution of Zn. The differentially expressed metal transporters and changes in subcellular distributions and Cd chemical forms were associated with Cd and Zn uptake and translocation. In summary, the application of Ca-K caused changes in gene expression, Cd and Zn uptake, translocation, and subcellular distribution.

Keywords: Cd chemical forms, Cd/Zn interaction, gene expression, metal transporters, subcellular distribution, Triticum polonicum.

Received: June 20, 2019; Revised: December 2, 2019; Accepted: December 19, 2019; Prepublished online: September 4, 2020; Published online: September 7, 2020Show citation

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SHUAI, W., YAO, Q., CHEN, X., CHENG, Y., XIAO, X., ZENG, J., ... WANG, Y. (2020). Cadmium and zinc uptake and translocation in dwarf Polish wheat seedlings as affected by calcium and potassium combination. Biologia plantarum, 64, 642-652. doi: 10.32615/bp.2019.162.
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    References

    1. Ahmad, P., Abdel Latef, A.A., Abd-Allah, E.F., Hashem, A., Sarwat, M., Anjum, N.A., Gucel, S.: Calcium and potassium supplementation enhanced growth, osmolyte secondary metabolite production, and enzymatic antioxidant machinery in cadmium-exposed chichpea (Cicer arietinum L.). - Front. Plant Sci. 7: 513, 2016. Go to original source...
    2. Ander, S., Huber, W.: Differential expression analysis for sequence count data. - Genome Biol. 11: R106, 2010. Go to original source...
    3. Cheng, Y., Wang, C., Chai, S., Shuai, W., Sha, L., Zhang, H., Kang, H., Fan, X., Zeng, J., Zhou, Y., Wang, Y.: Ammonium N influences the uptakes, translocations, subcellular distributions and chemical forms of Cd and Zn to mediate the Cd/Zn interactions in dwarf polish wheat (Triticum polonicum L.) seedlings. - Chemosphere 193: 1164-1171, 2018. Go to original source...
    4. Clemens, S.: Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants. - Biochimie 88: 1707-1719, 2006. Go to original source...
    5. Connorton, J.M., Jones, E.R., Rodríguez-Ramiro, I., Fairweather Tait, S., Uauy, C., Balk, J.: Wheat vacuolar iron transporter TaVIT2 transports Fe and Mn and is effective for biofortification. - Plant Physiol. 174: 2434-2444, 2017. Go to original source...
    6. Curie, C., Cassin, G., Couch, D., Divol, F., Higuchi, K., Jean, M.L., Misson, J., Schikora, A., Czernic, P., Mari, S.: Metal movement within the plant: contribution of nicotianamine and yeallow stripe 1-like transporters. - Ann. Bot. 103: 1-11, 2009. Go to original source...
    7. Evens, N.P., Buchner, P., Williams, L.E., Hawkesford, M.J.: The role of ZIP transporters and group F bZIP transcription factors in the Zn-deficiency response of wheat (Triticum aestivum). - Plant J. 92: 291-304, 2017. Go to original source...
    8. Han, Y., Sa, G., Sun, J., Shen, Z., Zhao, R., Ding, M., Deng, S., Lu, Y., Zhang, Y., Shen, X., Chen, S.: Overexpression of Populus euphratica xyloglucan endotransglucosylase/ hydrolase gene confers enhanced cadmium tolerance by the restriction of root cadmium uptake in transgenic tobacco. - Environ. exp. Bot. 100: 74-83, 2014. Go to original source...
    9. Hart, J., Welch, R.M., Norvell, W.A., Clarke, J.M., Kochian, L.V.: Zinc effects on cadmium accumulation and partitioning in near-isogenic lines of durum wheat that differ in grain cadmium concentration. - New Phytol. 167: 391-401, 2005. Go to original source...
    10. Hart, J., Welch, R.M., Norvell, W.A., Kochian, L.V.: Transport interactions between cadmium and zinc in roots of bread and durum wheat seedlings. - Physiol. Plant. 116: 73-78, 2002. Go to original source...
    11. Kobayashi, T., Nishizawa, N,K.: Iron uptake, translocation and regulation in higher plants. - Annu. Rev. Plant Biol. 63: 131-152, 2012. Go to original source...
    12. Lai, H.: Subcellular distribution and chemical forms of cadmium in Impatiens walleriana in relation to its phytoextraction potential. - Chemosphere 138: 370-376, 2015. Go to original source...
    13. Li, H., Luo, N., Li, Y., Cai, Q., Li, H., Mo, C., Wong, M.: Cadmium in rice: transport mechanisms, influencing factors, and minimizing measures. - Environ. Pollut. 224: 622-630, 2017. Go to original source...
    14. Li, J., Fu, Y., Pike, S., Bao, J., Tian, W., Zhang, Y., Chen, C., Zhang, Y., Li, H., Huang, J., Li, L., Schroeder, J.I., Gassmann, W., Gong, J.: The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance. - Plant Cell 22: 1633-1646, 2010. Go to original source...
    15. Li, L., Chen, O.S., McVey Ward, D., Kaplan, J.: CCC1 is a transporter that mediates vacuolar iron storage in yeast. - J. biol. Chem. 276: 29515-29519, 2001. Go to original source...
    16. Li, T., Tao, Q., Shohag, M.J.I., Yang, X., Sparks, D.L., Liang, Y.: Root cell wall polysaccharides are involved in cadmium hyperaccumulation in Sedum alfredii. - Plant Soil 389: 387-399, 2015. Go to original source...
    17. Lux, A., Martinka, M., Vaculik, M., White, P.J.: Root responses to cadmium in the rhizosphere: a review. - J. exp. Bot. 62: 21-37, 2011. Go to original source...
    18. Mariotti, A.: Natural 15N abundance measurements and atmospheric nitrogen standard calibration. - Nature 311: 251-252, 1984. Go to original source...
    19. Mortazavi, A., Williams, B.A., McCue, K., Schaeffer, L., Wold, B.: Mapping and quantifying mammalian transcriptomes by RNA-Seq. - Nat. Methods 5: 621-628, 2008. Go to original source...
    20. Nan, Z., Li, J., Zhang, J., Cheng, G.: Cadmium and zinc interactions and their transfer in soil-crop system under actual field conditions. - Sci. total Environ. 285: 187-95, 2002. Go to original source...
    21. Park, J., Song, W., Ko, D., Eom, Y., Hansen, T.H., Schiller, M., Lee, T.G., Martinoia, E., Lee, Y.: The phytochelatin transporters AtABCC1 and AtABCC2 mediate tolerance to cadmium and mercury. - Plant J. 69: 278-288, 2012. Go to original source...
    22. Peng, F., Wang, C., Cheng, Y., Zeng, J., Kang, H., Fan, X., Sha, N., Zhang, H., Zhou, Y., Wang, Y.: Cloning and characterization of TpNRAMP3, a metal transporter from polish wheat (Triticum polonicum L.). - Front. Plant Sci. 9: 1354, 2018b. Go to original source...
    23. Peng, F., Wang, C., Zhu, J., Zeng, J., Kang, H., Fan, X., Sha, L., Zhang, H., Zhou, Y., Wang, Y.: Expression of TpNRAMP5, a metal transporter from Polish wheat (Triticum polonicum L.), enhances the accumulation of Cd, Co and Mn in transgenic Arabidopsis plants. - Planta 247: 1395-1406, 2018a. Go to original source...
    24. Perrier, F., Yan, B., Candaudap, F., Pokrovsky, O.S., Gourdain, E., Meleard, B., Bussière, S., Coriou, C., Robert, T., Nguyen, C., Corny, J.Y.: Variability in grain cadmium concentration among durum wheat cultivars: impact of aboveground biomass partitioning. - Plant Soil 404: 307-320, 2016. Go to original source...
    25. Preston, T., Owens, N.J.P.: Interfacing an automatic elemental analyser with an isotope ratio mass sepectrometer: the potential for fully automated total nitrogen and nitrogen-15 analysis. - Analyst 108: 971-977, 1983. Go to original source...
    26. Sasaki, A., Yamaji, N., Yokosho, K., Ma, J.F.: Nramp5 is a major transporter responsible for manganese and cadmium uptake in rice. - Plant Cell 24: 2155-2167, 2012. Go to original source...
    27. Sharma, S.S., Dietz, K.J., Mimura, T.: Vacuolar compart-mentalization as indispensable component of heavy metal detoxification in plants. - Plant Cell Environ. 39: 1112-1126, 2016. Go to original source...
    28. Siddiqui, M.H., Al-Whaibi, M.H., Sakran, A.M., Basalah, M.O., Ali, H.M.: Effect of calcium and potassium on antioxidant system of Vicia faba L. under cadmium stress. - Int. J. mol. Sci. 13: 6604-6619, 2012. Go to original source...
    29. Song, Z., Duan, C., Guo, L., Yang, Y., Feng, Y., Ma, R., Yu, M.: Potassium contributes to zinc stress tolerance in peach (Prunus persica) seedling by enhancing photosynthesis and the antioxidant defense system. - Genet. mol. Res. 14: 8338-8835, 2015. Go to original source...
    30. Sun, Q., Wang, X., Ding, S., Yuan, X.: Effects of interactions between cadmium and zinc on phytochelation and glutathione production in wheat (Triticum aestivum L.). - Environ. Toxic. 20: 195-201, 2005. Go to original source...
    31. Takahashi, R., Ishimaru, Y., Shimo, H., Ogo, Y., Senoura, T., Nishizawa, N.K., Nakanishi, H.: The OsHMA2 transporter is involved in root-to-shoot translocation of Zn and Cd in rice. - Plant Cell Environ. 35: 1948-1957, 2012.
    32. Wang, J., Yuan, J., Yang, Z., Huang, B., Zhou, Y., Xin, J., Gong, Y., Yu, H.: Variation in cadmium accumulation among 30 cultivars and cadmium subcellular distribution in 2 selected cultivars of water spinach (Ipomoea aquatica Forsk.). - J. Agr. Food Chem. 57: 8942-8949, 2009. Go to original source...
    33. Wang, M., Xu, Q., Yu, J., Yuan, M.: The putative Arabidopsis zinc transporter ZTP29 is involved in the response to salt stress. - Plant mol. Biol. 73: 467-479, 2010. Go to original source...
    34. Wang, P., Deng, X., Huang, Y., Fang, X., Zhang, J., Wan, H., Yang, C.: Comparison of subcellular distribution and chemical forms of cadmium among four soybean cultivars at young seedlings. - Environ. Sci. Pollut. Res. 22: 19584-19595, 2015. Go to original source...
    35. Wang, X., Cheng, Y., Shuai, W., Zeng, J., Kang, H., Fan, X., Sha, L., Zhang, H., Wang, Y., Zhou, Y.: Nitrate N influences the accumulations and subcellular distributions of Cd and Zn to mediate the Cd/Zn interactions in dwarf Polish wheat (Triticum polonicum L.) seedling. - Soil Sci. Plant Nutr. 65: 137-147, 2019. Go to original source...
    36. Wang, X., Shi, M., Hao, P., Zheng, W., Cao, F.: Alleviation of cadmium toxicity by potassium supplementation involves various physiological and biochemical features in Nicotiana tabacum L. - Acta Physiol. Plant. 39: 132, 2017a. Go to original source...
    37. Wang, X., Zhi, J., Liu, X., Zhang, H., Liu, H., Xu, J.: Transgenic tobacco plants expressing a P1B-ATPase gene from Populus tomentosa Carr. (PtoHMA5) demonstrate improved cadmium transport. - Int. J. Biol. Macromol. 113: 655-661, 2018. Go to original source...
    38. Wang, Y., Wang, X., Wang, C., Peng, F., Wang, R., Xiao, X., Zeng, J., Kang, H., Fan, X., Sha, L., Zhang, H., Zhou, Y.: Transcriptomic profiles reveal the interactions of Cd/Zn in dwarf Polish wheat (Triticum polonicum L.) roots. - Front. Physiol. 8: 168, 2017b. Go to original source...
    39. Wang, Y., Wang, X., Wang, C., Wang, R., Peng, F., Xiao, X., Zeng, J., Fan, X., Kang, H., Sha, L., Zhang, H., Zhou, Y.: Proteomic profiling of the interactions of Cd/Zn in the roots of dwarf polish wheat (Triticum polonicum L.). - Front. Plant Sci. 7: 01378, 2016. Go to original source...
    40. Wiwart, M., Suchowilska, E., Kandler, W., Sulyok, M., Groenwald, P., Krska, R.: Can polish wheat (Triticum polonicum L.) be an interesting gene source for breeding wheat cultivars with increased resistance to Fusarium head blight? - Genet. Resour. Crop Evol. 60: 2359-2373, 2013. Go to original source...
    41. Wu, F., Dong, J., Qian, Q., Zhang, G.: Subcellular distribution and chemical form of Cd and Cd-Zn interaction in different barley genotypes. - Chemosphere 60: 1437-1446, 2005. Go to original source...
    42. Wu, H., Cheng, C., Du, J., Liu, H., Cui, Y., Zhang, Y., He, Y., Chu, C., Feng, Z., Li, J., Ling, H.: Co-overexpressing FIT with AtbHLH38 and AtbHLE39 in Arabidopsis-enhanced cadmium tolerance via increased cadmium sequestration in roots and improved iron homeostasis of shoots. - Plant Physiol. 158: 790-800, 2012. Go to original source...
    43. Xin, J., Huang, B., Dai, H., Liu, A., Zhou, W., Liao, K.: Characterization of cadmium uptake, translocation, and distribution in young seedling of two hot pepper cultivars that differ in fruit cadmium concentration. - Environ. Sci. Pollut. Res. 21: 7449-7456, 2014. Go to original source...
    44. Xu, L., Wang, Y., Liu, W., Wang, J., Zhu, X., Zhang, K., Yu, R., Wang, R., Xie, Y., Zhang, W., Gong, Y., Liu, L.: De novo sequencing of root transcriptome reveals complex cadmium-responsive regulatory networks in radish (Raphanus sativus L.). - Plant Sci. 236: 313-323, 2015. Go to original source...
    45. Yang, C., Juang, K.W.: Alleviation effects of calcium and potassium on cadmium rhizotoxicity and absorption by soybean and wheat roots. - J. Plant Nutr. Soil Sci. 178: 748-754, 2015. Go to original source...
    46. Zhang, Y., Xu, Y., Yi, H., Gong, J.: Vacuolar membrane transporters OsVIT1 and OsVIT2 modulate iron translocation between flag leaves and seeds in rice. - Plant J. 72: 400-410, 2012. Go to original source...
    47. Zhao, Z., Xi, M., Jiang, G., Liu, X., Bai, Z., Huang, Y.: Effects of IDSA, EDDS and EDTA on heavy metals accumulation in hydroponically grown maize (Zea mays, L.). - J. Hazard. Mater. 181: 455-459, 2010. Go to original source...
    48. Zhao, Z.Q., Zhu, T.G., Li, H.Y., Smith, S.E., Smith, F.A.: Effects of forms and rates of potassium fertilizers on cadmium uptake by two cultivars of spring wheat (Triticum aestivum L.). - Environ. Int. 29: 973-978, 2003.

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