Biologia plantarum, 1970 (vol. 12), issue 6
Article
The role of indoleacetaldehyde in IAA production from tryptophan by plants and by their epiphytic bacteria
E. Libbert, Roswitha Schröder
Biologia plantarum 12:369-377, 1970 | DOI: 10.1007/BF02922298
Tryptophan, tryptamine, or indolepyruvic acid were applied to 2 systems: a bacterial (pea stem sections containing the epiphytic bacteria) and a plant system (pea stem sections under sterile conditions).In the plant system, the production of indoleacetic acid and indoleethanol (tryptophol) from each applied indole derivative is clearly reduced by the aldehyde reagents bisulfite and dimedon, respectively. Indoleacetaldehyde is chromatographically detected after alkaline liberation from its bisulfite addition product.In the bacterial system, the production of indoleacetic acid and indoleethanol is likewise reduced by bisulfite and dimedon. However,...
Comparative electrophoretic investigation of some enzymes in extracts from different growth zones ofVicia faba L. Root
J. Sahulka
Biologia plantarum 12:378-381, 1970 | DOI: 10.1007/BF02922299
Activities of five enzymes were followed in electrophoreograms of extracts from three growth zones of broad bean root. With ascorbate oxidase (L-ascorbate: oxygen oxidoreductase, E.C. 1.10.3.3) one new fraction was found in the electrophoreograms of extracts from the maturation zone, electrophoreograms of α-glucan phosphorylase (α-l,4-glucan: orthophosphate glucosyltransferase, E.C. 2.4.1.1), amylase (α-l,4-glucan 4-glucanohydrolase, E.C. 3.2.1.1), sucrose glucosyltransferase (disaccharide glucosyltransferase, E.C. 2.4.1.7) and leucine aminopeptidase (L-leucyl-peptide hydrolase, E.C. 3.4.1.1) were identical for all three investigated...
Labeling of antibodies with radioactive isotopes in plant virus serology
E. Jermoljev, J. Pozděna, J. Balcar
Biologia plantarum 12:382-387, 1970 | DOI: 10.1007/BF02922300
Antibodies isolated from antiserum against plant viruses were labeled with the isotope35S as follows: the mixture of antibodies with radioactive cysteine hydrochloride was allowed to stand for half an hour, run on a Sephadex G-25 column and individual fractions were collected. Sephadex G-50 bed was equilibrated and washed with saline (0,85 % NaCl) phosphate buffer (0,01 m) pH 7,2. Fractions showing the highest radioactivity and at the same time the most evident serological reaction were combined and used as35S labeled antibodies. The labeled antibodies were used for rubbing leaves; the leaves were afterwards incubated, washed,...
The application of growth regulators for the physiological emasculation of rye(secale cereale l.) flowers
Z. Sladký
Biologia plantarum 12:388-394, 1970 | DOI: 10.1007/BF02922301
The present paper deals with the possibility of using growth regulators for the physiological emasculation of rye flowers and utilizes our knowledge of the higher sensibility of stamens to the auxin level. By means of bioassays it has been found that a relatively low content of auxins and a definite level of gibberellins and inhibitors are characteristic for normal rye spikes at the time of stamen differentiation. The higher level of auxins and expressive inhibitions occur in the later course of pistil differentiation.Two sprayings of plants with mixed solutions of MH and NAA and two further sprayings with a solution of NAA only before the stamen...
Effect of decreased rate of water absorption on water balance of leaf tissue
Jana Pospíšilová
Biologia plantarum 12:395-400, 1970 | DOI: 10.1007/BF02922302
The influence of decrease of water absorption rate on the transpiration rate and the development of water saturation deficit (WSD) was studied on the leaf segments of kale. Solutions of polyethyleneglycol (0-25m, 0-50m, O-75m and 1-00m) and mannitol (0-50m) were used as osmotic agents.The rate of water absorption decreased to zero when the concentration of polyethyleneglycol was 0-25m. At a concentration higher than 0-50m, water from the tissue diluted the external solution. The transpiration rate of samples affected by polyethyleneglycol or mannitol was only a little lower than that of control samples. WSD was noticeably increased only in the...
A leaf disc method for determination of tobacco mosaic virus and potato x-virus in leaves by indirect serological reaction using35s
J. Pozděna, E. Jermoljev
Biologia plantarum 12:401-404, 1970 | DOI: 10.1007/BF02922303
A method for determination of potato X-virus and tobacco mosaic virus in infected tobacco leaves was tested. The leaves are rubbed with isolated antibodies against homologous viruses and after 30 minutes incubation in a humid chamber at room temperature are washed with fresh distilled water 3 times. The leaves are afterwards rubbed with35S-labeled pig gamma globulin against rabbit gamma globulin. The most convenient radioactivity of the labeled pig gamma globulin was 100 (µCi. Leaf discs 5 mm in diameter are punched out after half an hour incubation and their radioactivity is measured on a 27π window methane flow counter. The leaves...
Investigation on maize (corn) mosaic virus (mosaico rayado del maiz) and its spread in cuba
J. Smrž, B. A. Kvíčala, N. Blanco, L. Acosta
Biologia plantarum 12:405-413, 1970 | DOI: 10.1007/BF02922304
Maize mosaic and stunt, occurring to a considerable degree under Cuban field conditions, was determined as a virus disease, transmissible by the corn planthopperPeregrinus maidis Ashm. Negative results were obtained in the experiments in which aphidsRhopalosiphum maidis Fitch, served as vectors or when transmission was realized mechanically using the sap of infected plants. The incubation period in the infected plants fluctuated between 15 and 27 days. Besides maize, the virus disease could be transmitted also to sorghum(Sorghum vulgare Pees.) and to weed plants of the grassRottboellia exaltata L. It follows from the results...
Aphids can acquire beet yellows virus from leaves covered with a membrane
J. BrČák, J. Polák
Biologia plantarum 12:414-418, 1970 | DOI: 10.1007/BF02922305
Beet yellows virus was transmitted by wingless aphidsMyzus persicae (Sulz.) after 16 hrs acquisition feeding on leaves covered with a Parafilm M membrane. Effectiveness of the virus transmission was decreased approximately to half of the original value by acquisition of the virus through the membrane. No virus was transmitted to healthy beet seedlings after 6 or 16 hrs continuous feeding on a concentrated suspension of the purified virus or on crude juice extracted from an infected plant (suspensions contained 15% of sucrose).
Brief Communications
Responses of stomata of barley and maize to phenylmercuric acetate
O. Majerník
Biologia plantarum 12:419-423, 1970 | DOI: 10.1007/BF02922306
A reduction of stomatal aperture in light was found in leaves of maize after they had been treated with 10"3-5 m phenylmercuric acetate (PMA). Complete closure of the stomata in darkness was prevented, whilst there was total closure in the controls. Higher PMA concentrations had bigger effects. The relative water content (RWC) of barley tissues was slightly reduced 12 hours after treatment with PMA. The transpiration rate observed on PMA-treated barley plants was lower in light and higher in darkness than in untreated plants. Water saturation deficit (WSD) was higher by about 5%, and water holding capacity (WHC) lower (25%) than in untreated plants....
Reproduction of sugar beet mosaic and tobacco mosaic viruses in anthocyanized beet plants
Věra Sosnová
Biologia plantarum 12:424-427, 1970 | DOI: 10.1007/BF02922307
During our studies on the interaction of anthocyanins and plant virus diseases, reproduction of sugar beet mosaic (SBMV) and tobacco mosaic viruses (TMV) was investigated. Experiments were carried out in leaves of sugar beet,Beta vulgaris cv. Dobrovicka N and its spontaneous anthocyanized mutant. SBMV induces a systemic infection while TMV is responsible for primary local symptoms in sugar beet leaves only. Our quantitative analyses onAmaranthus caudatus L. andChenopodium quinoaWilid. showed a significant decrease in concentration of SBMV in juice extracted from anthocyanized beet plants as compared with extracts from normal green...
The precursor of indolylacrylic acid inlens culinaris med. seedlings
M. Hofikger
Biologia plantarum 12:428-430, 1970 | DOI: 10.1007/BF02922308
The 3-(3-indolyl)-aorylic acid previously found in ether or ethylaoetate extracts of lentil seedlings is proved to be formed during extraction from an indolic precursor.