Biologia plantarum 55:178-182, 2011 | DOI: 10.1007/s10535-011-0026-6

Effect of aluminum on the in vitro activity of acid phosphatases of four potato clones grown in three growth systems

L. A. Tabaldi1, D. Cargnelutti2, G. Y. Castro1, J. F. Gonçalves1, R. Rauber1, D. A. Bisognin3, M. R. C. Schetinger2, F. T. Nicoloso1,*
1 Departamento de Biologia, Centro de Ciências Naturais e Exatas (CCNE), Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brasil
2 Departamento de Química, CCNE, UFSM, Santa Maria, RS, Brasil
3 Departamento de Fitotecnia, Centro de Ciências Rurais, UFSM, Santa Maria, RS, Brasil

The aim of this study was to assess the effect of aluminum on the in vitro activity of acid phosphatases (APases) of four potato clones, Macaca and Dakota Rose (Al-sensitive), and SMIC148-A and Solanum microdontum (Al-tolerant), grown in vitro, in hydroponics or in a greenhouse. The enzyme was assayed in vitro in the presence of 0, 1.85, 3.70, 5.55 and 7.40 mM Al. In plantlets grown in vitro, root APases were inhibited by Al in all clones, while shoot APases were inhibited by Al in S. microdontum and Dakota Rose and increased in Macaca at all Al concentrations. In plantlets grown in hydroponics, root APases increased in Macaca at 1.85 mM Al, whereas decreased at all Al levels in S. microdontum. In greenhouse plantlets, root APases decreased at 7.40 mM Al in S. microdontum and SMIC148-A, and at 3.70, 5.55 and 7.40 mM Al in Dakota Rose. Shoot APases decreased in Macaca and SMIC148-A. Conversely, in Dakota Rose, APases increased at 1.85 and 3.70 mM Al. These results show that the effect of Al toxicity on in vitro APase activity depends not only on Al availability but also on the plant organ, genetic background, and the growth conditions. Therefore, it suggests that acid phosphatases activity assessed in vitro might not be a good parameter to validate the screening for adaptation of potato clones to Al toxicity.

Keywords: abiotic stress; phosphorus metabolism; Solanum tuberosum; Solanum microdontum; tolerance

Received: May 9, 2009; Accepted: December 15, 2009; Published: March 1, 2011Show citation

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Tabaldi, L.A., Cargnelutti, D., Castro, G.Y., Gonçalves, J.F., Rauber, R., Bisognin, D.A., Schetinger, M.R.C., & Nicoloso, F.T. (2011). Effect of aluminum on the in vitro activity of acid phosphatases of four potato clones grown in three growth systems. Biologia plantarum, 55(1), 178-182. doi: 10.1007/s10535-011-0026-6.
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