Biologia Plantarum 63: 757-764, 2019 | DOI: 10.32615/bp.2019.153

Proline metabolism-related gene expression in four potato genotypes in response to drought stress

Y. LIU1,2, L. WANG1,3, Y. LI4, X. LI5, J. ZHANG1,4,*
Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University,
1 Lanzhou 730070, P.R. China
2 College of Agronomy, Gansu Agricultural University, Lanzhou 730070, P.R. China
3 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, P.R. China
4 College of Horticulture, Gansu Agricultural University, Lanzhou 730070, P R. China
5 Qingyang Academy of Agricultural Sciences, Qingyang 745000, P.R. China

Drought severely limits potato yield. The aim of this work was to study a response of plantlets of four potato genotypes to polyethylene glycol (PEG 6000)-induced drought stress in both physiological and molecular levels. The drought-tolerant and drought-sensitive genotypes were identified based on plantlet growth, chlorophyll content, lipid peroxidation, free proline content, and proline metabolism-related gene expressions. We found that PEG-induced stress increased malondialdehyde (MDA) and proline content, and drought-tolerant plantlets exhibited lower MDA and proline content than sensitive genotypes. In addition, PEG up-regulated ∆-1-pyrroline-5-carboxylate synthase (P5CS) and pyrroline- 5-carboxylate reductase (P5CR) gene expressions and down-regulated pyrroline dehydrogenase (PDH) and ∆-1-pyrroline-5-carboxylate dehydrogenase (P5CDH) gene expressions. Genotype B plantlets exhibited lower P5CS and P5CR expressions and higher PDH and P5CDH expressions compared with the other plantlets. The results suggest that significant cultivar differences among potato plantlets in response to PEG-induced drought stress are exhibited in root length, MDA content, proline accumulation, and proline metabolism-related gene expressions.

Keywords: chlorophyll, in vitro cultivation, malondialdehyde, PEG-induced drought stress, Solanum tuberosum.

Received: December 21, 2018; Revised: January 17, 2019; Accepted: February 26, 2019; Published online: December 9, 2019Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
LIU, Y., WANG, L., LI, Y., LI, X., & ZHANG, J. (2019). Proline metabolism-related gene expression in four potato genotypes in response to drought stress. Biologia plantarum, 63, 757-764. doi: 10.32615/bp.2019.153.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files

    Download fileLiu6049 Suppl.pdf

    File size: 487.87 kB

    References

    1. Arnon, D. I.: Copper enzymes in isolated chloroplasts, polyphenol oxidase in Beta vulgaris L. - Plant Physiol. 24: 1-15, 1949. Go to original source...
    2. Bates, L.S., Waldren, R.P., Teare, I.D.: Rapid determination of free proline for water-stress studies. - Plant Soil 39: 205-207, 1973. Go to original source...
    3. Boyer, J. S.: Plant productivity and environment. - Science 218: 443-448, 1982. Go to original source...
    4. Delauney, A. J., Verma, D. P. S.: Proline biosynthesis and osmoregulation in plants. - Plant J. 4: 215-223, 1993. Go to original source...
    5. Demiral, T., Türkan, İ.: Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. - Environ. exp. Bot. 53: 247-257, 2005. Go to original source...
    6. Ellis, D., Franco, N., Gomez, R., Rossel, G., Manrique, I., Panta, A., Salas, A., Silvestre, R., Vargas, F., Vollmer, R., Zea, B.: The genebank at the International Potato Center: a global asset to help food security. - Amer. J. Potato Res. 92: 186-191, 2015.
    7. Gopal, J., Iwama, K., Jitsuyama, Y.: Effect of water stress mediated through agar on in vitro growth of potato. - In Vitro cell. dev. Biol. Plant. 44: 221-228, 2008. Go to original source...
    8. Gupta, B., Huang, B.: Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. - Int. J. Genomics 2014, 2014. Go to original source...
    9. Hodges, D.M., DeLong, J.M., Forney, C.F., Prange, R.K.: Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. - Planta 207: 604-611, 1999. Go to original source...
    10. Kishor, K., Polavarapu, B., Sreenivasulu, N.: Is proline accumulation per se correlated with stress tolerance or is proline homeostasis a more critical issue? - Plant Cell Environ. 37: 300-311, 2014. Go to original source...
    11. Kubala, S., Wojtyla, Ł., Quinet, M., Lechowska, K., Lutts, S., Garnczarska, M.: Enhanced expression of the proline synthesis gene P5CSA in relation to seed osmopriming improvement of Brassica napus germination under salinity stress. - J. Plant Physiol. 183: 1-12, 2015. Go to original source...
    12. Larher, F., Leport, L., Petrivalsky, M., Chappart, M.: Effectors for the osmoinduced proline response in higher plants. - Plant Physiol. Biochem. 31: 911-922, 1993.
    13. Liu, J., Zhu, J. K.: Proline accumulation and salt-stress-induced gene expression in a salt-hypersensitive mutant of Arabidopsis. - Plant Physiol. 114: 591, 1997. Go to original source...
    14. Livak, K.J., Schmittgen, T D.: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. - Methods 25: 402-408, 2001. Go to original source...
    15. Murashige, T., Skoog, F.: A revised medium for rapid growth and bio assays with tobacco tissue cultures. - Physiol. Plant. 15: 473-497, 1962. Go to original source...
    16. Nayyar, H., Walia, D. P.: Water stress induced proline accumulation in contrasting wheat genotypes as affected by calcium and abscisic acid. - Biol. Plant. 46: 275-279, 2003. Go to original source...
    17. Rai, M.K., Kalia, R.K., Singh, R., Gangola, M.P., Dhawan, A.K.: Developing stress tolerant plants through in vitro selection - an overview of the recent progress. - Environ. exp. Bot. 71: 89-98, 2011. Go to original source...
    18. Rampino, P., Pataleo, S., Gerardi, C., Mita, G., Perrotta, C.: Drought stress response in wheat: physiological and molecular analysis of resistant and sensitive genotypes. - Plant Cell Environ. 29: 2143-2152, 2006. Go to original source...
    19. Spoljarevic, M., Agic, D., Lisjak, M., Gumze, A., Wilson, I. D., Hancock, J. T., Teklic, T.: The relationship of proline content and metabolism on the productivity of maize plants. - Plant Signal Behav. 6: 251-257, 2011. Go to original source...
    20. Tewary, P., Sharma, A., Raghunath, M., Sarkar, A.: In vitro response of promising mulberry (Morus sp.) genotypes for tolerance to salt and osmotic stresses. - Plant Growth Regul. 30: 17-21, 2000. Go to original source...
    21. Turan, S., Tripathy, B.C.: Salt and genotype impact on antioxidative enzymes and lipid peroxidation in two rice cultivars during de-etiolation. - Protoplasma 250: 209-222, 2013. Go to original source...
    22. Wang, S.-T., Pan, Y.-Y., Liu, C.-C., Chuang, L.-T., Chen, C.-N. N.: Characterization of a green microalga UTEX 2219-4: effects of photosynthesis and osmotic stress on oil body formation. - Bot. Studies. 52: 305-312, 2011.
    23. Wang, X.-L., Li, F.-M., Jia, Y., Shi, W.-Q.: Increasing potato yields with additional water and increased soil temperature. - Agr. Water. Manage. 78: 181-194, 2005. Go to original source...
    24. Wang, X. S., Han, J. G.: Changes of proline content, activity, and active isoforms of antioxidative enzymes in two alfalfa cultivars under salt stress. - J. integr. Agr. 8: 431-440, 2009. Go to original source...
    25. Xue, X., Liu, A., Hua, X.: Proline accumulation and transcriptional regulation of proline biothesynthesis and degradation in Brassica napus. - BMB Rep. 42: 28-34, 2009. Go to original source...

    Return to the content