Biologia plantarum 55:379-382, 2011 | DOI: 10.1007/s10535-011-0059-x

Genetic diversity of Eucalyptus hybrids estimated by genomic and EST microsatellite markers

F. B. Cupertino1, J. B. Leal1, R. X. Corrêa1, F. A. Gaiotto1,*
1 Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, Bahia, Brazil

The knowledge of breeding impacts on the genetic diversity of hybrids of Eucalyptus is crucial to the exploration of genetic resources. We estimated genetic polymorphic parameters of 112 hybrids of Eucalyptus spp. using 10 genomic simple sequence repeats (SSR) markers and 10 expressed sequence tags (EST) microsatellite markers. According to Student's t-test, there were no significant differences between genomic SSR and EST-SSR markers. Our results also revealed high polymorphism in the hybrids analyzed, indicating that both markers are appropriate for use in genetic breeding programs.

Keywords: allelic diversity; eucalypt; SSR

Received: November 23, 2009; Accepted: March 31, 2010; Published: June 1, 2011Show citation

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Cupertino, F.B., Leal, J.B., Corrêa, R.X., & Gaiotto, F.A. (2011). Genetic diversity of Eucalyptus hybrids estimated by genomic and EST microsatellite markers. Biologia plantarum, 55(2), 379-382. doi: 10.1007/s10535-011-0059-x.
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    References

    1. Akkak, A., Scariot, V., Torello Marinoni, D., Boccacci, P., Beltramo, C., Botta, R.: Development and evaluation of microsatellite markers in Phoenix dactylifera L. and their transferability to other Phoenix species. - Biol. Plant. 53: 164-166, 2009. Go to original source...
    2. Brondani, R.P.V., Williams, E.R., Brondani, C., Grattapaglia, D.: A microsatellite-based consensus linkage map for species of Eucalyptus and a novel set of 230 microsatellite markers for the genus. - BMC Plant Biol. 6: 1-16, 2006. Go to original source...
    3. Campinhos, E.: Sustainable plantations of high-yield shape Eucalyptus trees for production of fiber: the Aracruz case. - New Forest. 17: 129-143, 1999. Go to original source...
    4. Cho, Y.G., Ishii, T., Temnykh, S., Chen, X., Lipovich, L., Mccouch, S.R., Park, W.D., Ayres, N., Cartinhour, S.: Diversity of microsatellites derived from genomic libraries and GenBank sequences in rice (Oryza sativa L.). - Theor. appl. Genet. 100: 713-722, 2000. Go to original source...
    5. Eldridge, K., Davidson, J., Harwood, C., Wyk, G.V. (ed.): Eucalypt Domestication and Breeding. - Oxford University Press, New York 1994.
    6. Ferreira, M.E., Grattapaglia, D. (ed.): Introdução ao Uso de Marcadores Moleculares em Análises Genéticas. [Introduction to the usage of molecular markers in genetic analysis]. - Embrapa-Cenargen, Brasília 1998. [In Port.]
    7. Gupta, P.K., Balyan, H.S., Sharma, P.C., Ramesh, B.: Microsatellites in plants: a new class of molecular markers. - Curr. Sci. 70: 45-54, 1996.
    8. Jones, M.E., Stokoe, R.L., Cross, M.J., Scott, L.J., Maguire, T.L., Shepherd, M.: Isolation of microsatellite loci from spotted gum (Corymbia variegata), and cross-species amplification in Corymbia and Eucalyptus. - Mol. Ecol. Notes. 1: 276-278, 2001. Go to original source...
    9. Kantartzi, S.K., Ulloa, M., Sacks, E., Stewart, J.M.: Assessing genetic diversity in Gossypium arboreum L. cultivars using genomic and EST-derived microsatellites. - Genetica 136: 141-147, 2009. Go to original source...
    10. Kirst, M., Cordeiro, C.M., Rezende, G.D.S.P., Grattapaglia, D.: Power of microsatellite markers for fingerprint and parentage analysis in Eucalyptus grandis breeding populations. - J. Heredity 96: 161-166, 2005. Go to original source...
    11. Liewlaksaneeyanawin, C., Ritland, C.R., El-Kassaby, Y.A., Ritland, K.: Single-copy, species transferable microsatellite markers developed from loblolly pine. - Theor. appl. Genet. 109: 361-369, 2004. Go to original source...
    12. Marcucci-Poltri, S.N., Zelener, N., Rodriguez-Traverso, J., Gelid, P., Hopp, H.E.: Selection of a seed orchard of Eucalyptus dunnii based on genetic diversity criteria calculated using molecular markers. - Tree Physiol. 23: 625-632, 2003. Go to original source...
    13. Marques, C.M., Brondani, R.P.V., Grattapaglia, D., Sederoff, R.: Conservation and synteny of SSR loci and QTLs for vegetative propagation in four Eucalyptus species. - Theor. appl. Genet. 105: 474-478, 2002. Go to original source...
    14. Peleg, Z., Fahima, T., Abbo, S., Krugman, T., Saranga, Y.: Genetic structure of wild emmer wheat populations as reflected by transcribed versus anonymous SSR markers. - Genome 51: 187-195, 2008. Go to original source...
    15. Poke, F.S., Vaillancourt, R.E., Potts, B.M., Reid, J.B.: Genomic Research in Eucalyptus. - Genomics 125: 79-101, 2005. Go to original source...
    16. Powell, W., Machray, G.C., Provan, J.: Polymorphism revealed by simple sequence repeat. - Trends Plant Sci. 1: 215-222, 1996. Go to original source...
    17. Varshney, R.K., Graner, A., Sorrells, M.E.: Genomic microsatellite markers in plants: features and applications. - Trends Biotechnol. 23: 48-55, 2005. Go to original source...

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