Biologia plantarum 57:231-237, 2013 | DOI: 10.1007/s10535-012-0273-1

Characterization of the GLP13 gene promoter in Arabidopsis thaliana

L. Yang1, T. Li1, S. C. Zhang1, G. L. Gao1, C. W. Yang1,*
1 Guangdong Key Laboratory of Biotechnology and Plant Development, College of Life Science, South China Normal University, Guangzhou, P.R. China

In transgenic plants, for many applications it is important that the inserted genes are expressed in a tissue-specific manner. This in turn could help better understanding their roles in plant development. Germin-like proteins (GLPs) play diverse roles in plant development and defense responses. In order to understand the functions and regulation of the GLP13 gene, its promoter (762 bp) was cloned and fused with a β-glucuronidase (GUS) reporter gene for transient expression in Arabidopsis thaliana and tobacco (Nicotiana tabacum cv. K326). Histochemical analysis of the transgenic plants showed that GUS was specifically expressed in vascular bundles predominantly in phloem tissue of all organs in Arabidopsis. Further analyses in transgenic tobacco also identified similar GUS expression in the vascular bundles.

Keywords: germin-like proteins; GUS activity; Nicotiana tabacum; phloem; tobacco; transgenic plants
Subjects: gene promoter; germin-like proteins; GUS activity; phloem; tobacco; transgenic plant

Received: February 15, 2012; Accepted: July 10, 2012; Published: June 1, 2013Show citation

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Yang, L., Li, T., Zhang, S.C., Gao, G.L., & Yang, C.W. (2013). Characterization of the GLP13 gene promoter in Arabidopsis thaliana. Biologia plantarum, 57(2), 231-237. doi: 10.1007/s10535-012-0273-1.
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