Biologia plantarum 57:313-324, 2013 | DOI: 10.1007/s10535-012-0274-0

Differential ozone sensitivity interferes with cadmium stress in poplar clones

A. Castagna1,*, D. Di Baccio2, R. Tognetti3,*, A. Ranieri1, L. Sebastiani2
1 Dipartimento di Biologia delle Piante Agrarie, Università di Pisa, Pisa, Italy
2 BioLabs, ISV, Scuola Superiore Sant'Anna, Pisa, Italy
3 Dipartimento di Bioscienze e Territorio, Università degli Studi del Molise, Pesche, Italy

Information on plant responses to combined ozone and cadmium stresses are scarce and limited to herbaceous species. In this research, two poplar clones (I-214 and Eridano), differently sensitive to O3, were grown for 5 weeks in pots supplied with 0, 53.5, and 160.5 mg(Cd) kg-1 (soil d.m.) and then exposed to 15-d O3 fumigation (0.06 mm3 dm-3, 5 h a day). The effects of the two stressors, alone or in combination, on Cd, Ca, Fe, and Zn accumulation in above-nad below-ground organs, photosynthesis, leaf pigments, and accumulation of H2O2 and NO were investigated. Cadmium induced a reduction in stomatal conductance and a significant accumulation of H2O2 and NO in both clones nad negatively affected the carotenoid content in I-214. Ozone, on the other hand, counteracted Cd accumulation in the above-ground organs and significantly increased the xanthophyll de-epoxidation state indicating photoinhibition in O3-treated plants. Surprisingly, O3 alone or in combination with Cd decreased H2O2 accumulation in I-214. The NO production was generally stimulated by Cd, whereas it decreased following O3 exposure in I-214. The overall data indicate that Cd and O3 induced clone specific responses. Moreover, when they were applied in combination, antagonistic rather than synergistic effects were observed.

Keywords: carotenoids; hydrogen peroxide; nitric oxide; net photosynthetic rate; Populus spp.; stomatal conductance; xanthophyll cycle
Subjects: ozone; cadmium; poplar; carotenoids; hydrogen peroxide; nitric oxide; photosynthetic rate; stomatal conductance; calcium; iron; zinc; growth analysis; chlorophyll; xanthophylls

Received: March 21, 2012; Accepted: June 18, 2012; Published: June 1, 2013Show citation

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Castagna, A., Baccio, D., Tognetti, R., Ranieri, A., & Sebastiani, L. (2013). Differential ozone sensitivity interferes with cadmium stress in poplar clones. Biologia plantarum, 57(2), 313-324. doi: 10.1007/s10535-012-0274-0.
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