Biologia plantarum 57:375-379, 2013 | DOI: 10.1007/s10535-012-0284-y

Cold hardening and sucrose treatment improve cryopreservation of date palm meristems

L. Fki1,*, N. Bouaziz1, O. Chkir1, R. Benjemaa-Masmoudi1, A. Rival2, R. Swennen3,4, N. Drira1, B. Panis3
1 Laboratory of Plant Biotechnology, Faculty of Sciences, University of Sfax, Sfax, Tunisia
2 UMR DIADE, CIRAD, Montpellier, France
3 Laboratory of Tropical Crop Improvement, Catholic University Leuven, Leuven, Belgium
4 Bioversity International, Catholic University Leuven, Leuven, Belgium

Date palm (Phoenix dactylifera L.) cv. Khenizi caulogenic meristems were initiated from achlorophyllous leaves excised from in vitro shoot cultures and then proliferated on a specific culture medium supplemented with 70 g dm-3 sucrose. Regeneration rates obtained when using standard vitrification, droplet-vitrification, and encapsulation-vitrification protocols reached 26.7, 60.0, and 40.0 %, respectively. Only explants smaller than 3 mm in diameter were found to survive cryogenic treatments. Sucrose preculture, cold hardening and loading solution pretreatments showed significant effects on regeneration rates. Moreover, our results indicate that both sucrose preculture and cold acclimation of explants increased proline content. Cryopreservation of date palm tissue with high proliferation capacity can directly benefit large scale micropropagation projects.

Keywords: caulogenesis; in vitro; micropropagation; Phoenix dactylifera; proline; vitrification
Subjects: cold hardening; temperature low; cryopreservation; in vitro culture; date palm; proline; vitrification; saccharides

Received: March 28, 2012; Accepted: August 22, 2012; Published: June 1, 2013Show citation

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Fki, L., Bouaziz, N., Chkir, O., Benjemaa-Masmoudi, R., Rival, A., Swennen, R., Drira, N., & Panis, B. (2013). Cold hardening and sucrose treatment improve cryopreservation of date palm meristems. Biologia plantarum, 57(2), 375-379. doi: 10.1007/s10535-012-0284-y.
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