Effects of CO₂ concentration on acclimatization and physiological responses of two cultivars of carob tree

M. L. Osório, S. Gonçalves, J. Osório, A. Romano

Biologia plantarum 49:161-167, 2005 | DOI: 10.1007/s10535-005-1167-2

This study reports survival and physiological responses of micropropagated Ceratonia siliqua L. cvs. Galhosa and Mulata plants during ex vitro acclimatization under ambient (AC; 330 μmol mol^-1) or elevated (EC; 810 μmol mol^-1) CO₂ concentration and a photosynthetic photon flux density of 125 μmol m^-2 s^-1. CO₂ enrichment during acclimatization did not improve survival rate that was around 80 % for both treatments. Eight weeks after ex vitro transplantation, photosynthetic capacity and apparent quantum yield in acclimatized leaves were higher in comparison...

Direct differentiation of somatic embryos on cotyledons of Azadirachta indica

A. Gairi, A. Rashid

Biologia plantarum 49:169-173, 2005 | DOI: 10.1007/s10535-005-0173-8

Somatic embryos regenerated in high-frequency (up to 100 %) on immature cotyledons of Azadirachta indica at a low concentration of thidiazuron (TDZ; 0.5 μM). Regeneration occurred exclusively from abaxial surface. Frequency of regeneration declined with the age of the cotyledons: on semimature cotyledons regeneration occurred only from some regions of the abaxial surface and on mature cotyledons it was confined to corners. However, an increase in concentration of TDZ to 1.0 μM improved the regeneration response. Higher regenerative capacity of immature cotyledons was due to presence of milk in immature fruits because regeneration response...

Efficient regeneration of Brassica napus L. hypocotyls and genetic transformation by Agrobacterium tumefaciens

P. Jonoubi, A. Mousavi, A. Majd, A.H. Salmanian, M. Jalali Javaran, J. Daneshian

Biologia plantarum 49:175-180, 2005 | DOI: 10.1007/s10535-005-5180-2

An efficient system for shoot regeneration and Agrobacterium-mediated gene transfer into Brassica napus was developed through the modification of the culture conditions. Different concentrations of benzyladenine (1.5, 3.0 and 4.5 mg dm^-3) and thidiazuron (0.0, 0.15 and 0.30 mg dm^-3) were evaluated for shoot regeneration of 7, 14 and 21-d-old hypocotyl explants. Maximum shoot regeneration frequency was obtained in 21-d-old explants using 4.5 mg dm^-3 benzyladenine and 0.3 mg dm^-3 thidiazuron. Under above culture condition, the highest percentage of shoot regeneration frequency was 200 %. Agrobacterium-infected...

The use of mutagens to increase the efficiency of the androgenic progeny production in Solanum nigrum

D. Kopecký, J. Vagera

Biologia plantarum 49:181-186, 2005 | DOI: 10.1007/s10535-005-1186-z

Pollen embryogenesis was successfully induced in Solanum nigrum L. (2n=6×=72). Stimulation of androgenesis expressed as the frequency of androgenic responsive anthers was observed after 10 and 20 mM ethyl methanesulphonate (EMS), 10 and 20 mM sodium azide (NaN₃) and 0.2 mM N-nitroso-N-methylurea (MNU) treatment applied on seeds for 24 h. The frequency of androgenesis on the medium with sucrose was higher than on the medium with maltose. Androgenic regenerants originated also in the anthers collected from donor plants where survival after mutagenic treatment was lower than 50 %. Green haploid (3x), aneuploid (to 8x) and dihaploid (6x)...

Expression of Lupinus albus PR-10 proteins during root and leaf development

M. P. Pinto, A. Ribeiro, A. P. Regalado, C. Rodrigues-Pousada, C. P. P. Ricardo

Biologia plantarum 49:187-193, 2005 | DOI: 10.1007/s10535-005-7193-2

Based on the NH₂-terminal sequence of three PR-10 isoforms previously identified in Lupinus albus leaves and a conserved amino-acid region in the PR-10 proteins from leguminosae, a pair of oligonucleotides was designed and used to amplify the corresponding cDNA fragment from a L. albus leaves cDNA library. A fragment of DNA of 200 bp was isolated from the polymerase chain reaction (PCR) mixture and subsequently used to screen the cDNA library. A cDNA coding for a PR-10 protein of 158 amino acid residues was cloned and sequenced. Subsequent studies involving Northern and Western blot analysis have shown that the PR-10 protein...

The relationship between vernalization requirement and frost tolerance in substitution lines of wheat

I. T. Prášil, P. Prášilová, K. Pánková

Biologia plantarum 49:195-200, 2005 | DOI: 10.1007/s10535-005-5200-2

Two sets of wheat (Triticum aestivum L.) substitution lines for the homoeologous group 5 chromosomes, 5A, 5B and 5D, carrying vernalization genes (Vrn-A1, Vrn-B1, Vrn-D1) were used to study the relationship between vernalization requirement and winter survival, with respect to the induction and maintenance of frost tolerance. Substitution lines carrying dominant Vrn loci substituted from the spring cultivars Zlatka (5A), Chinese Spring (5D) and the alternative cultivar Česká Přesívka (5B) into three different winter wheat backgrounds, Vala, Košútka and Zdar, showed lower winter survival by 20, 36, and 41 % for substitutions of...

Oxidations of various substrates and effects of the inhibitors on purified mitochondria isolated from Kalanchoë pinnata

H. T. K. Hong, A. Nose, S. Agarie

Biologia plantarum 49:201-208, 2005 | DOI: 10.1007/s10535-005-1208-x

Kalanchoë pinnata mitochondria readily oxidized succinate, malate, NADH, and NADPH at high rates and coupling. The highest respiration rates usually were observed in the presence of succinate. The high rate of malate oxidation was observed at pH 6.8 with thiamine pyrophosphate where both malic enzyme (ME) and pyruvate dehydrogenase were activated. In CAM phase III of K. pinnata mitochondria, both ME and malate dehydrogenase (MDH) simultaneously contributed to metabolism of malate. However, ME played a main function: malate was oxidized via ME to produce pyruvate and CO₂ rather than via MDH to produce oxalacetate...

Ultrastructural changes in rhizome parenchyma of Polypodium vulgare during dehydration with or without abscisic acid pretreatment

E. Zenkteler, A. Bagniewska-Zadworna

Biologia plantarum 49:209-214, 2005 | DOI: 10.1007/s10535-005-0214-3

Common polypody (Polypodium vulgare L.) belongs to desiccation-tolerant ferns. The structure of storage parenchyma of their rhizome was examined by transmission electron microscopy after dehydration and subsequent rewetting. Analysis revealed that treatment with supplemental abscisic acid resulted in protection of cells against ultrastructural damage compared to untreated ones. Dehydration rate appears to modify the ability of rhizome parenchyma to stand water stress.

Changes in phytohormone contents in chickpea seeds germinating under lead or zinc stress

Ö. Atici, G. Ağar, P. Battal

Biologia plantarum 49:215-222, 2005 | DOI: 10.1007/s10535-005-5222-9

The present work describes the changes that take place in phytohormone contents in germinating chickpea (Cicer arietinum cv. Aziziye-94) seeds in response to heavy metal stress. For this aim, endogenous abscisic acid (ABA), gibberellic acid (GA₃), zeatin (Z) and zeatin riboside (ZR) contents were followed for 24, 48 and 72 h in chickpea seeds germinating at the concentrations of 0.1, 1.0 and 5.0 mM Pb or 0.1, 1.0 and 10 mM Zn. The results showed that Pb and Zn significantly delayed and impeded the germination of chickpea seeds. The negative effect of Pb on germination was higher than that of Zn. Further, Pb increased ABA and Z contents...

Hormone-induced protection of sunflower photosynthetic apparatus against copper toxicity

G. Ouzounidou, I. Ilias

Biologia plantarum 49:223-228, 2005 | DOI: 10.1007/s10535-005-3228-y

The effects of excess Cu as affected by the application of exogenous hormones (gibberellic acid - GA₃ and indole-3-acetic acid - IAA) with respect to sunflower (Helianthus annuus L.) growth, physiology, and metabolism were studied. Application of 100 μM IAA lessened the toxic effects of 80 μM Cu in roots indicating greater root length and root hair formation, while addition of 100 μM GA₃ ameliorated the toxic effect mainly to the shoot. The content of photosynthetic pigments significantly declined under Cu stress, whereas application of hormones led to a substantial preservation of chlorophylls and carotenoids....

UV-B radiation and cadmium induced changes in growth, photosynthesis, and antioxidant enzymes of cyanobacterium Plectonema boryanum

S. M. Prasad, M. Zeeshan

Biologia plantarum 49:229-236, 2005 | DOI: 10.1007/s10535-005-0236-x

UV-B (0.4 W m^-2) irradiation and cadmium (2 and 8 μM) treatments separately inhibited the survival, growth, pigment content, and photosynthetic electron transport in Plectonema boryanum. Phycocyanin was the main target to UV-B and Cd and it was followed by chlorophyll a and carotenoids. UV-B and Cd caused strong inhibition on activities of photosystem 2 (PS2) and the whole electron transport chain, whereas photosystem 1 (PS1) was the least affected. UV-B and Cd treatment accelerated respiration, lipid peroxidation, and the activities of superoxide dismutase and catalase. However, enhancement in catalase activity was considerably...

Cadmium uptake, localization and detoxification in Zea mays

M. Wójcik, A. Tukiendorf

Biologia plantarum 49:237-245, 2005 | DOI: 10.1007/s10535-005-7245-7

Cadmium uptake, translocation and localization in maize roots and shoots at the tissue and cellular level were investigated. Metal accumulation in plant organs as well as symptoms of Cd toxicity were closely correlated with an increase in Cd concentration applied (5 - 300 μM). Most of the metal taken up was retained in roots, mainly inside the cells of endodermis, pericycle and central cylinder parenchyma. Accumulation of phytochelatins and related peptides also depended on Cd concentration in the nutrient solution.

Ecophysiological characterization of carnivorous plant roots: Oxygen fluxes, respiration, and water exudation

L. Adamec

Biologia plantarum 49:247-255, 2005 | DOI: 10.1007/s10535-005-7255-5

Various ecophysiological investigations on carnivorous plants in wet soils are presented. Radial oxygen loss from roots of Droseraceae to an anoxic medium was relatively low 0.02 - 0.07 μmol(O₂) m^{- 2} s^-1 in the apical zone, while values of about one order of magnitude greater were found in both Sarracenia rubra roots and Genlisea violacea traps. Aerobic respiration rates were in the range of 1.6 - 5.6 μmol kg^-1 (f.m.) s^-1 for apical root segments of seven carnivorous plant species and 0.4 - 1.1 μmol kg^-1 (f.m.) s^-1 for Genlisea traps. The...

An efficient in vitro method for mass propagation of Tylophora indica

M. Faisal, M. Anis

Biologia plantarum 49:257-260, 2005 | DOI: 10.1007/s10535-005-7260-8

A protocol of high frequency shoot organogenesis and plant establishment from stem derived callus has been developed for Tylophora indica (Burm. f.) Merrill. - an endangered medicinal plant. Callus was developed on Murashige and Skoog (MS) medium supplemented with 10 μM 2,4,5-trichlorophenoxy acetic acid (2,4,5-T). Multiple shoot induction was achieved from the surface of the callus after transferring onto shoot induction medium. The highest rate (80 %) of shoot multiplication was achieved on MS medium containing 5.0 μM kinetin. The developed shoots rooted best on half-strength MS medium supplemented with 0.5 μM indole-3-butyric acid...

The effect of a short heat treatment on the in vitro induced androgenesis in Silene latifolia ssp. alba

D. Šafářová, D. Kopecký, J. Vagera

Biologia plantarum 49:261-264, 2005 | DOI: 10.1007/s10535-005-1264-2

The effect of a short heat treatment in combination with different culture medium composition on the efficiency of in vitro induced androgenesis in Silene latifolia ssp. alba was studied. The heat shocks (33 and 37°C) were applied for 1, 3, and 5 d. The best androgenic response was observed at 25°C and after a one-day treatment at 33°C. All other treatments reduced androgenic response. Among different media compositions tested, the most satisfactory results were obtained on BMS medium supplemented with 6-benzylaminopurine (0.5 mg dm^-3) and sucrose. The green, albino and chimeric, only female, plants were regenerated....

Importance of glucose-6-phosphate dehydrogenase in taxol biosynthesis in Taxus chinensis cultures

L. J. Yu, W. Z. Lan, C. Chen, Y. Yang, Y. P. Sun

Biologia plantarum 49:265-268, 2005 | DOI: 10.1007/s10535-005-5268-8

The roles of glucose-6-phosphate dehydrogenase (G6PDH) in paclitaxel production were investigated in cell suspension cultures of Taxus chinensis. In the normal cultures, the trend of G6PDH activity was similar to that of cell growth. Addition of glutamate increased G6PDH activity, while dehydroepiandrosterone (DHEA) decreased G6PDH activity. In elicitor-treated cultures, cell growth was depressed, while G6PDH activity and taxol production were enhanced compared with the control. Glutamate recovered the depression of cell growth, and resulted in further increase in G6PDH activity and taxol production. Contrarily, DHEA exacerbated the depression...

Water-use efficiency and carbon isotope discrimination of Acacia ampliceps and Eucalyptus camaldulensis at different soil moisture regimes under semi-arid conditions

J. Akhter, K. Mahmood, M. A. Tasneem, K. A. Malik, M. H. Naqvi, F. Hussain, R. Serraj

Biologia plantarum 49:269-272, 2005 | DOI: 10.1007/s10535-005-0272-6

Acacia ampliceps Maslin and Eucalyptus camaldulensis Dehnh. were grown for one year in lysimeters at three soil moisture regimes: 100 % (well-watered), 75 % (medium-watered) and 50 % (low-watered) of total plant available water. Biomass yield of both species increased with increase in soil moisture. Water-use efficiency (WUE) of E. camaldulensis decreased and that of A. ampliceps increased markedly with decrease in available soil moisture. A. ampliceps showed 4 - 5 times more biomass yield than E. camaldulensis grown at similar soil moisture. A. ampliceps showed almost 5, 9 and 12 times higher WUE than...

Alleviation of salt stress by low dose γ-irradiation in rice

M.-H. Baek, J.-H. Kim, B. Y. Chung, J.-S. Kim, I. S. Lee

Biologia plantarum 49:273-276, 2005 | DOI: 10.1007/s10535-005-3276-3

The effects of salt stress on the growth, photosynthesis, and antioxidative ability of the rice (Oryza sativa L.) plants raising from γ-irradiated seeds were investigated using two cultivars, Ilpumbyeo and Sanghaehyanghyella. The 50 and 100 mM NaCl solutions caused a remarkable decrease of the early germination rate and seedling growth. However, the salt stress-induced inhibition of the growth was significantly alleviated in the γ -irradiated plants. The chlorophyll contents and the effective quantum yield of photosystem 2 (Φ _{PS 2}) were lower in the NaCl-treated plants than in the control ones, while the non-photochemical...

A new medium formulation for in vitro rooting of carob tree based on leaf macronutrients concentrations

S. Gonçalves, P. J. Correia, M. A. Martins-Loução, A. Romano

Biologia plantarum 49:277-280, 2005 | DOI: 10.1007/s10535-005-7280-4

Experiments were performed to optimize the macronutrients concentrations for in vitro rooting of Ceratonia siliqua micropropagated shoots. Several dilutions of Murashige and Skoog (MS) medium were tested: full-strength MS, half-strength MS (1/2MS), and 1/2MS + full N. The frequency of in vitro rooting was enhanced when the 1/2MS was used (50 % rooted shoots). Mature leaves from 20 - 30 year-old carob trees and from 2 year-old micropropagated plants were collected and the concentrations of macronutrients (N, P, K, Ca, Mg) assessed. Based on the mineral composition of the leaves a new medium was formulated and compared with the previous...

In vitro regeneration of mature Pinus sylvestris buds stored at freezing temperatures

U. Andersone, G. Ievinsh

Biologia plantarum 49:281-284, 2005 | DOI: 10.1007/s10535-005-1284-y

Changes of morphogenic competence in mature P. sylvestris L. buds due to frozen storage were investigated. The highest callus formation was registered on explants stored at -18°C for three months, but on explants stored for five months, it was also higher than in the control. Budding and development of needles in vitro was observed only for buds frozen three to five months. Peroxidase activity was lowest in these buds. In contrast, polyphenol oxidase activity in bud tissues continually increased during frozen storage. Within 10 months of frozen storage the content of starch and sugars in resting buds changed. It may be concluded that...

Growth and ion uptake in Annona muricata and A. squamosa subjected to salt stress

V. M. Passos, N. O. Santana, F. C. Gama, J. G. Oliveira, R. A. Azevedo, A. P. Vitória

Biologia plantarum 49:285-288, 2005 | DOI: 10.1007/s10535-005-5288-4

The effects of treatment with NaCl (3, 100 and 300 mM) for 1, 2, 3 and 7 d on plant growth and ion accumulation were analyzed in 2-week and 8-week-old Annona muricata and A. squamosa plants. Fresh mass and root growth inhibition were directly related to the increase in salinity, particularly for A. squamosa. Two-weeks old seedlings were sensitive to 100 and 300 mM NaCl particularly after 7 d, whereas 8-week-old plants were shown to be more resistant to NaCl even at 300 mM NaCl. Na⁺ and Cl^- mostly accumulated in young leaves. Our results suggest that A. squamosa is more sensitive than A. muricata...

Carbendazim alleviates effects of water stress on chickpea seedlings

Smita, H. Nayyar

Biologia plantarum 49:289-291, 2005 | DOI: 10.1007/s10535-005-0291-3

Carbendazim (methyl-2-benzimidazole carbamate) promoted root growth of chickpea (Cicer arietinum L.) seedlings subjected to polyethylene glycol (PEG, osmotic potential -0.5 MPa) induced water stress. The relative water content, membrane stability index, 2,3,5-triphenyltetrazolium chloride reduction and contents of some osmolytes (proline, sucrose, glucose and fructose) enhanced significantly while the contents of lipid peroxides and hydrogen peroxide diminished effectively by addition of 0.05 % carbendazim into PEG solution.

Effect of arsenic on some physiological parameters in bean plants

N. Stoeva, M. Berova, Z. Zlatev

Biologia plantarum 49:293-296, 2005 | DOI: 10.1007/s10535-005-3296-z

The objective of the study was to investigate the effect of different arsenic concentrations on some physiological parameters of bean (Phaseolus vulgaris L.) cultivars Plovdiv 10 and Prelom in the early growth phases. Seedlings, grown in sand with Hoagland-Arnon nutrient solution in a climatic box, were treated with 0, 2, 5 mg(As) dm^-3 as Na₃AsO₄ (pH 5.5). After 5 d of As treatment, the changes in leaf gas-exchange, water potential, chlorophyll and protein contents, peroxidase activity and lipid peroxidation in roots were recorded. Physiological analysis showed a minor negative effect of arsenic at concentration...

Effects of different N-sources on growth, nutritional status, chlorophyll content, and photosynthetic parameters of shoots of the apple rootstock MM 106 cultured in vitro

T. E. Sotiropoulos, G. N. Mouhtaridou, T. Thomidis, V. Tsirakoglou, K. N. Dimassi, I. N. Therios

Biologia plantarum 49:297-299, 2005 | DOI: 10.1007/s10535-005-7299-6

The effects of five different N-sources (KNO₃ + NH₄NO₃ = control, KNO₃, NH₄NO₃, NH₄H₂PO₄, L-alanine) on growth, nutritional status, chlorophyll (Chl) content, and photosynthetic parameters of the apple rootstock MM 106 shoots cultured in vitro were investigated. In comparison to all the other treatments, control explants grown on a MS medium containing KNO₃ + NH₄NO₃ had the highest fresh mass, Chl content, net photosynthetic rate, transpiration rate, and stomatal conductance.

The effects of NaCl on growth, water relations, osmolytes and ion content in Kochia prostrata

G. Karimi, M. Ghorbanli, H. Heidari, R. A. Khavari Nejad, M. H. Assareh

Biologia plantarum 49:301-304, 2005 | DOI: 10.1007/s10535-005-1304-y

The effects of NaCl (0, 50, 100, 150 and 200 mM) on growth, water relations, glycinebetaine, free proline, ion contents, stomata number and size of Kochia prostrata (L.) Schard were determined. Shoot and root fresh and dry matter, root and shoot length, relative growth rate, net assimilation rate, relative water content, water use efficiency, soluble sugars and glycinebetaine contents were not changed at low NaCl concentrations, but they were significantly decreased at 200 mM NaCl. The K⁺, Mg²⁺ and Ca²⁺ contents, water potential, chlorophyll a+b and carotenoides contents, and stomata number and size were...

Plant water status, H₂O₂ scavenging enzymes, ethylene evolution and membrane integrity of Cicer arietinum roots as affected by salinity

S. Kukreja, A. S. Nandwal, N. Kumar, S. K. Sharma, S. K. Sharma, V. Unvi, P. K. Sharma

Biologia plantarum 49:305-308, 2005 | DOI: 10.1007/s10535-005-5308-4

The chickpea genotype, CSG-8962 was raised in screenhouse to study salinity induced changes in ethylene evolution, antioxidative defence system and membrane integrity in relation to changes in plant water and mineral content. At vegetative stage (60 d after sowing), the plants were exposed to single saline irrigation (0, 2.5, 5.0 and 10.0 dS m^-1). Sampling was done 3 d after saline treatments. The other sets of treated plants were re-irrigated with water and sampled after further 3 d. The Ψ_w of leaf and Ψ_s of leaf and roots decreased from -0.47 to -0.61 MPa, -0.67 to -1.23 MPa and from -0.57 to -0.95 MPa, respectively,...

Regulation of metabolic pathways PVY-RNA biosynthesis in tobacco: Host's RNA degradation

L. Šindelář, M. Šindelářová

Biologia plantarum 49:309-312, 2005 | DOI: 10.1007/s10535-005-0312-2

Tobacco plants infected with the potato virus Y (PVY) were studied during the acute-infection period. The control enzymes of metabolic pathway of host's RNA degradation tending to biosynthesis of PVY-RNA, its coarse/fine regulation and content of host's RNA were monitored. Activities of ribonucleases, phosphomonoesterases and phosphodiesterases in both the crude homogenates and the partially purified enzyme preparations from the diseased leaves were markedly increased when compared to the tissues from healthy plants. The curves of enzyme activities positively correlated with the multiplication curve of the PVY and negatively correlated with the decreased...

Use of a simple semiquantitative method for appraisal of green fluorescent protein gene expression in transgenic tobacco plants

M. Hraška, S. Rakouský, T. Kocábek

Biologia plantarum 49:313-316, 2005 | DOI: 10.1007/s10535-005-3316-z

We have applied a simple method for evaluation of gfp gene expression in plants using a CCD camera and computerized processing of images. Transgenic tobacco plants were obtained by Agrobacterium tumefaciens-mediated transfer of plasmid T-DNA bearing a m-gfp5-ER sequence governed by the 35S promoter together with the nptII selectable marker gene. Presence of the gfp gene in plants was confirmed by a polymerase chain reaction method. Mean brightness values measured using image analysis software showed differences between transgenic and control plants and suggest the possibility of rapid selection of transgenic individuals...

Effects of short-term soil flooding on stomata behaviour and leaf gas exchange in barley plants

R. Y. Yordanova, A. N. Uzunova, L. P. Popova

Biologia plantarum 49:317-319, 2005 | DOI: 10.1007/s10535-005-7319-6

Exposure of barley plants (Hordeum vulgare L.) to soil flooding for 2 to 24 h reduced the net photosynthetic rate and transpiration rate. Stomatal conductance also decreased in flooded plants. Stomatal closure started within 2 - 6 h and stomata remained closed up to 24 h of treatment.

Khan, J.A., Dijkstra, J. (ed.): Plant Viruses as Molecular Pathogens. - Food Products Press, an imprint of The Haworth Press, New York - London - Oxford 2002. 537 pp. Hardcover USD 129.95, ISBN 1-56022-894-6; softcover USD 59.95, ISBN 1-56022-895-4

M. Šindelářová

Biologia plantarum 49:168, 2005 | DOI: 10.1007/s10535-005-8168-z

Benech-Arnold, R.L., Sánchez, R.A. (ed.): Handbook of Seed Physiology. Application to Agriculture. - The Haworth Press, New York - London - Oxford 2004. 480 pp. USD 49.95. ISBN 1-56022-928-4

L. Moravcová

Biologia plantarum 49:174, 2005 | DOI: 10.1007/s10535-005-4174-4

Campbell, A., Anderson, W.W, Jones, E.W. (ed.): Annual Review of Genetics. Volume 38. - Annual Reviews Inc., Palo Alto, California, 2004, 878 pp. ISBN 0-8243-1238-4

T. Gichner

Biologia plantarum 49:194, 2005 | DOI: 10.1007/s10535-005-4194-0

Schekman, R., Goldstein L., Rossant, J. (ed.): Annual Review of Cell and Developmental Biology. Volume 20. - Annual Reviews Inc., Palo Alto 2004, 961 pp. ISBN 0-8243-3120-6

T. Gichner

Biologia plantarum 49:246, 2005 | DOI: 10.1007/s10535-005-6246-x