Biologia plantarum - Latest articles

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Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during droughtOriginal Papers

R. M. Acevedo, E. H. Avico, O. A. Ruiz, P. A. Sansberro

Biologia plantarum 62:89-96, 2018 | DOI: 10.1007/s10535-017-0754-3

Reverse transcription of RNA followed by real-time quantitative PCR (qPCR) is to date the most reliable method for gene expression studies. However, to control the errors introduced along the numerous experimental procedures, it requires a normalization using internal reference genes with stable expression. To address this issue, nine candidate reference genes were investigated in Ilex paraguariensis leaves subjected to water stress. To facilitate the selection, we analysed the real-time qPCR data with three different software programs. The obtained results support the conclusion that RNA polymerase associated protein rtf1 homolog (RTF)...

Knockout mutants of Arabidopsis thaliana β-galactosidase. Modifications in the cell wall saccharides and enzymatic activitiesOriginal Papers

M. Moneo-Sánchez, L. Izquierdo, I. Martín, J. Hernández-Nistal, L. Albornos, B. Dopico, E. Labrador

Biologia plantarum 62:80-88, 2018 | DOI: 10.1007/s10535-017-0739-2

This work studied the six β-galactosidases (BGALs) of the subfamily a1 of Arabidopsis, that have been proposed to play important roles in the cell wall remodelling during plant development, although their precise functions are still unknown. Knockout mutants bgal1, bgal2, bgal3, bgal4, bgal5, and bgal12 of Arabidopsis and their wild type (WT) plants were analysed to determine their morphology and composition of their cell walls. The gas chromatography and the Fourier transform infrared spectroscopy revealed differences between the mutants and their WT such as in the proportions of glucose,...

High irradiance sensitive phenotype of Arabidopsis hit2/xpo1a mutant is caused in part by nuclear confinement of AtHsfA4aOriginal Papers

H.-Y. Huang, K.-Y. Chang, S.-J. Wu

Biologia plantarum 62:69-79, 2018 | DOI: 10.1007/s10535-017-0753-4

In Arabidopsis, EXPORTIN1A (HIT2/XPO1A) and EXPORTIN1B (XPO1B) mediate the translocation of nuclear export sequence (NES)-bearing proteins from nucleus to cytoplasm. However, a mutation in HIT2/XPO1A but not in XPO1B induces sensitivity to high irradiance (HI). Arabidopsis thaliana heat stress elements A4a and A5 (AtHsfA4a and AtHsfA5) are involved in plant responses to HI and possess NESs; therefore, their nucleo-cytoplasmic partitioning was analyzed. In wild-type and xpo1b mutant cells, AtHsfA4a normally remained in the cytoplasm but became concentrated in the nucleus following exposure to HI, whereas AtHsfA5 was...

Overexpression of tomato WHIRLY protein enhances tolerance to drought stress and resistance to Pseudomonas solanacearum in transgenic tobaccoOriginal Papers

S.-Y. Zhao, G.-D. Wang, W.-Y. Zhao, S. Zhang, F.-Y. Kong, X.-C. Dong, Q.-W. Meng

Biologia plantarum 62:55-68, 2018 | DOI: 10.1007/s10535-017-0714-y

WHIRLY transcription factors play critical roles in responses to biotic and abiotic stresses, but their other biological functions remain unclear. In this study, SlWHY2, a member of the WHIRLY family, was isolated from Solanum lycopersicum. The role of SlWHY2 was studied using transgenic tobacco plants. Real-time quantitative polymerase chain reaction analysis showed that SlWHY2 expression was induced by polyethylene glycol, NaCl, salicylic acid, hydrogen peroxide, and bacterial pathogens. SlWHY2 overexpression in tobacco caused enhanced tolerance to drought stress, as indicated by lower accumulation of malondialdehyde...

Identification and functional analysis of anthocyanin biosynthesis genes in Phalaenopsis hybridsOriginal Papers

L. M. Wang, J. Zhang, X. Y. Dong, Z. Z. Fu, H. Jiang, H. C. Zhang

Biologia plantarum 62:45-54, 2018 | DOI: 10.1007/s10535-017-0763-2

Phalaenopsis species are among the most popular potted flowers for their fascinating flowers. When their whole-genome sequencing was completed, they have become useful for studying the molecular mechanism of anthocyanin biosynthesis. Here, we identified 49 candidate anthocyanin synthetic genes in the Phalaenopsis genome. Our results showed that duplication events might contribute to the expansion of some gene families, such as the genes encoding chalcone synthase (PeCHS), flavonoid 3'-hydroxylase (PeF3'H), and myeloblastosis (PeMYB). To elucidate their functions in anthocyanin biosynthesis, we conducted a global expression...

Characterization of Citrus WRKY transcription factors and their responses to phytohormones and abiotic stressesOriginal Papers

V. Vives-Peris, D. Marmaneu, A. Gómez-Cadenas, R. M. Pérez-Clemente

Biologia plantarum 62:33-44, 2018 | DOI: 10.1007/s10535-017-0737-4

WRKY transcription factors (TF) family is involved in a huge variety of plant processes, including seed germination, plant development, phytohormone signalling, and defence against both biotic or abiotic stresses. In this work, WRKY TF family has been characterized in citrus. In a first experiment, the relative expression of CsWRKYs was analyzed in shoots and roots of plants treated with abscisic acid (ABA), salicylic acid (SA), and methyl jasmonate (MeJA) under in vitro conditions. Expression of CsWRKYs was also determined in roots of commercial citrus rootstocks subjected to osmotic and salt stresses. A total amount of 50 CsWRKYs...

Identification, characterization, and expression of the SWEET gene family in Phalaenopsis equestris and Dendrobium officinaleOriginal Papers

T. Wang, Z. Song, W. L. Meng, L. B. Li

Biologia plantarum 62:24-32, 2018 | DOI: 10.1007/s10535-017-0750-7

Sugars are important molecules that function not only as primary metabolites, but also as nutrients and signal molecules in plants. The sugar transport protein genes family SWEET has been recently identified. The availability of the Dendrobium officinale and Phalaenopsis equestris genome sequences offered the opportunity to study the SWEET gene family in this two orchid species. We identified 22 and 16 putative SWEET genes, respectively, in the genomes of D. officinale and P. equestris using comprehensive bioinformatics analysis. Based on phylogenetic comparisons with SWEET proteins from Arabidopsis...

Gibberellin A₃ as an epigenetic determinant of global DNA hypo-methylation in tobaccoOriginal Papers

R. Manoharlal, G. V. S. Saiprasad, C. Ullagaddi, A. Kovařík

Biologia plantarum 62:11-23, 2018 | DOI: 10.1007/s10535-017-0738-3

Gibberellins (GAs) are a large family of tetracyclic diterpenoids, controlling important aspects of growth and development throughout the plant life cycle. To explore the possibility that gibberellin A₃ (GA₃) signalling induces epigenetic alteration(s), we carried out a field experiment study using Nicotiana tabacum as a model system. The GA₃ application on leaves resulted in increased plant-height, foliage density, leaf cell area, and trichome density. The plants exposed to GA₃ also exhibited: 1) increased chromatin de-condensation, 2) reduced global DNA methylation, 3) reduced...

Enhanced multiplication and improved ex vitro acclimatization of Decalepis arayalpathraOriginal Papers

Z. Ahmad, A. Shahzad, S. Sharma

Biologia plantarum 62:1-10, 2018 | DOI: 10.1007/s10535-017-0746-3

The proposed work describes a protocol for high-frequency in vitro regeneration through nodal segments and shoot tips in Decalepsis arayalpathra, a critically endangered medicinal liana of the Western Ghats. Nodal segments were more responsive than shoot tips in terms of shoot proliferation. Murashige and Skoog's (MS) basal medium supplemented with 5.0 μM 6-benzyladenine (BA) was optimum for shoot initiation through both the explants. Among different combinations of plant growth regulators and growth additive screened, MS medium added with 5.0 μM BA + 0.5 μM indole-3-acetic acid + 20.0 μM adenine sulphate effectuated the highest...