Measurement of respiratory CO₂ production of roots in an aquatic medium

Hana Končalová, G. Albrecht, J. Pokorný, E. M. Wiedenroth

Biologia plantarum 31:1-7, 1989 | DOI: 10.1007/BF02890673

Changes in pH, total alkalinity and O₂ concentration were followed in an aquatic medium with excised wheat roots (Tritkum aestivum L.). Concentrations of total inorganic carbon and free CO₂ were calculated from total alkalinity and pH according to carbonate equilibria. The total inorganic carbon was estimated by flow-injection infra-red gas analysis. Total alkalinity increased in the root medium during incubation. Respiratory CO₂ production was estimated best from the increase in total inorganic carbon measured with an infra-red gas analyser.

Protein and RNA Content and Synthesis in Embryos and Endosperms from Developing Triticum duram Seeds

Isa Gulu, M. Ceciua AnguillesI, C. Flores

Biologia plantarum 31:8-18, 1989 | DOI: 10.1007/BF02890674

Protein and RNA contents and synthesis were evaluated in the course of wheat grain (T. durum cv. Cappelli) development. Embryos and endosperms were considered separately during five phases from the 20th day after anthests until full ripenes was reached. No clean-cut changes were observed in the pattern of soluble proteins of the embryos. In the endosperms protein synthesis continues till the later phases and appears to be due to the albumin + globulin component. Screening of bands of endosperm proteins from electrophoresis indicates that the gliadins are synthesized early, with the exception of a Ω - gliadin. Glutelins with high relative molecular...

The Effect of Cadmium on Tobacco Cell Culture and the Selection of Potentially Cd-Resistant Cell lines

Eva Domažlická, Z. Opatrný

Biologia plantarum 31:19-27, 1989 | DOI: 10.1007/BF02890675

The effect of different concentrations of cadmium on the viability, cell division, and the total increase in the biomass of the VBI-O cell strain of tobacco (Nicotiana tabacumL., Virginia Bright Italia) was followed.The concentration of 10^-6 mol 1^-1 Cd²⁺ was fully tolerated by this strain, a nearly total inhibition of cell division and high cell mortality rate ocsicurred at the concentration of 10^-4 mol1{si-1} Cd²⁺.Following a long-term exposure of the culture to gradually increasing cadmium concentrations, seven cell lines able to grow on media containing 10^-4 mol 1^-1...

Mathematical Description of Water and Solution Flows in Segments of Maize Roots and in Branches of White Birch

J. Michalov

Biologia plantarum 31:28, 1989 | DOI: 10.1007/BF02890676

The need of the study of the transport of water molecules and of solutions in the biological system and shortcomings in the contemporary modelling of bioflows led us to the construction of a new mathematical model which would more precisely characterize the bioflows controlled by natural sources (for example the gradient of hydrostatic and osmotic pressure, of electric potential and of temperature) without limiting thermodynamic conditions. The model issues from the reality of the biosystem as a black box. It uses those input quantities which are attainable. It has been verified with white birch branches, the primary root and the roots of the first...

The Influence of Some Phenolic Compounds on Nodulation in Pigeonpea (Cajanus cajan(L.) Millsp.)

K. K. Dhir, Lalitha Rao

Biologia plantarum 31:44, 1989 | DOI: 10.1007/BF02890677

Studies on exogenous application of phenolic compoundsviz: p-hydroxybenzoic acid, resorcinol and chlorogenic acid each with concentration of 10^-4 M are done on the legume (Cajanus cajan (L.)Millsp.) AL-15. The effect of applied phenolic compounds as well as of structural differences in phenols indicate a marked influence of phenolic compounds in regulating growth processes in plants. Fresh and dry mass of various plant parts increased after foliar spray with phenols resulting in an improved harvest index. It is seen that phenols also play an important role in the initiation and development of nodules.

Search for Tissue Specific Plant Lectins A Preliminary Report

V. M. Haseena Beevi, P. Remani, R. Ankathil, K. K. Vijayan, T. Vijayakumar

Biologia plantarum 31:51, 1989 | DOI: 10.1007/BF02890678

Extract of the seeds ofAnona reticulata, Camellia sinensis, Bauhinia acuminata, Cassia tomentosa, Malus sylvestris, Trigonella foenumgraecum, Cephalandra indica, Lawsonia inermis, Anacardium occidentale, Mangifera indica, Nephelium litchi, Citrus lemoni, Aegle marmelos, Quassia amara, Mimusops elengi, Achras sapota, Datura stramonium, Thevetia nerifolia, Persea americana andCycas circinalis, were screened for lectin activity by haemagglutination and haemagglutination inhibition assays. Lectin-like activity was detected only in the seeds ofMangifera indica and Perseaamericana. The conventional methods for the isolation of...

Stimulation of Ethylene Production by Exogenous Spermidine in Detached Tobacco Leaves in the Light

S. Pennazio, P. Roggero

Biologia plantarum 31:58, 1989 | DOI: 10.1007/BF02890679

Exogenous supply of Spd and Spin stimulated ethylone production in detached tobacco leaves kept in the light. Stimulation, that was first detected after 9 but not 6 h of treatment, linearly increased with concentration and was maintained for several h after returning treated leaves to deionized water. Stimulation of ethylene production was prevented by AVG and Co²⁺ and was accompanied by increased activity of ACC synthase and ethylene-forming enzyme. Put, and other diamines, did not give any stimulatory effect. Stimulation was not accompanied by apparent damage of plasmalomina nor was it reversed by Ca⁺ or Put, suggesting that...

Preparation of Chloroplast DNA from Barley and Lettuce and Comparison of Restriction Fragments

Mercedes Martin, R. Tomás, María Cruz Izquierdo, C. Prádanos, B. Sabater

Biologia plantarum 31:67, 1989 | DOI: 10.1007/BF02890680

Chloroplast DNA from three barley cultivars and from one lettuce cultivar was prepared from chloroplasts isolated by Conventional differential centrifugation. Barley chloroplast DNA size was sensibly lower (130 kpb) than lettuce chloroplast DNA (150 kpb). Chloroplast DNAs from the three barley cultivars showed similar restriction fragment patterns after digestion with: BamHI, EcoRI or HindIII. The lettuce chloroplast DNA restriction pattern was very different from the barley chloroplast DNA restriction pattern.

Book Reviews

Ingrid TichÁ, J. TupÝ, F. PospIšil, J. Pazourek, Jarmila SolÁrovÁ

Biologia plantarum 31:75-88, 1989 | DOI: 10.1007/BF02890682

Influence of CO² on Stomatal Numbers

P. Apel

Biologia plantarum 31:72-74, 1989 | DOI: 10.1007/BF02890681

From nine different plant species grown at 1500 cm³ m^-3 CO₂ five responded with a significant increase in stomatal numbers per mm² as compared with plants grown under normal air conditions. Within a collection of twelve french bean cultivars remarkable cultivar differences with regard to the CO₂ enhancement effect on stomatal numbers was found.