Biologia plantarum, 1984 (vol. 26), issue 5

Article

Book review

Ingrid Tichá

Biologia plantarum 26:348, 1984 | DOI: 10.1007/BF02898570

Book review

Věra Hadačová

Biologia plantarum 26:357, 1984 | DOI: 10.1007/BF02898572

Book review

Ingrid Tichá

Biologia plantarum 26:363, 1984 | DOI: 10.1007/BF02898574

Book review

T. Gichner

Biologia plantarum 26:373, 1984 | DOI: 10.1007/BF02898576

Book review

Jana Pospíšilová, J. Challice, Eva Klozová, Jarmila Solárová, J. Čatský, M. Kaše, T. Gichner, J. Bříza, Kateřina Pánková, J. Velemínský, Vlasta Čatská, Danuše Hodáňová, Kateřina Pánková, Vlasta Čatská, M. Ondřej, F. J. Novák, Jiřina Švachulová

Biologia plantarum 26:387-398, 1984 | DOI: 10.1007/BF02898580

Original Papers

Biochemical studies of the regeneration of the root cuttings ofClerodendrum viscosum Vent

T. K. Prasad, P. M. Mehta, Y. S. Dave

Biologia plantarum 26:321, 1984 | DOI: 10.1007/BF02898566

The contents of reducing sugars, proteins and ascorbic acid and the activities of protease, peroxidase, catalase, indole acetic acid oxidase, ascorbic acid oxidase, succinic dehydrogenase and the rate of respiration were investigated in regenerating and non-regenerating parts ofClerodendrum viscosum Vent. root cuttings. All the above variables have registered higher in the regenerating parts, particularly at the distal end where roots appear, than in the non-regenerating parts of root cuttings. The contents of metabolites and the activities of enzymes in the presence of endogenous cytokinins at the proximal end and auxins at the distal end,...

Effects of oxygenation of castor bean cotyledons on proton and sucrose influx

Françoise de Bock

Biologia plantarum 26:327, 1984 | DOI: 10.1007/BF02898567

Some of the effects of oxygen concentration in the external medium bathing castor bean cotyledons were investigated.At 700 μM O2, the sucrose-dependent proton influx was dependent on external pH while sucrose influx was independent of pH between 4,5 and 6; proton influx and sucrose influx were both dependent on external sucrose concentration; both were saturated above 50 mM sucrose. The stoichiometry H+/S decreased with increasing pH and sucrose concentration. These data were consistent with the results obtained in stirred or aerated external medium.Oxygen consumption, acidification of the medium, sucrose-dependent proton influx...

Stimulation of adventitious bud Initiation by cyclic AMP and traumatic acid inTorenia stem segments

Shizufumi Tanimoto, Hiroshi Harada

Biologia plantarum 26:337, 1984 | DOI: 10.1007/BF02898568

Effects of cyclic AMP and traumatic acid on adventitious bud initiation inTorenia stem segments culturedin vitro were examined. Both the compounds stimulated bud initiation at relatively low concentrations. Simultaneous addition of an anticytokinin did not counteract the stimulatory action of the 2 chemicals. Their stimulatory effects were also clear when expiants were pre-incubated for 2 h with 1 μM of either of the 2 substances. The treatment with cyclic AMP and traumatic acid may favourably modify some physiological process which is triggered by excising expiants (wounding) and leads to adventitious bud initiation.

Growth-correlative effect of the root on the apical part of the epicotyl in pea seedlings regarding the IAA content and L-tryptophan aminotransferase and L-tryptophan dehydrogenase activities

Tan Hoang Minh, M. Kutáček, J. Šebánek

Biologia plantarum 26:342, 1984 | DOI: 10.1007/BF02898569

In five-day-old etiolated pea seedlings an increase in IAA content and in L-tryptophan aminotransferase (TAT) and L-tryptophan dehydrogenase (TDH) activities in the apical part of the epicotyl was found 12 h after root excision, which was followed by a decrease up to 48 h. In ten-day-old seedlings a continuous decrease in IAA, TAT and TDH levels in the apical part of the epicotyl was recorded up to 48 h after root excision. This indicates a growth-correlative effect of the root becoming evident in dependence on plant ontogenesis in changes of IAA content and activities of the above enzymes catalysing IAA synthesis.

The comparison of lectin spectra of certain representatives of the speciesPhaseolus vulgaris ssp.aborigineus of different geographical origin

Jiřina Švachulová, Eva Klozová, Věra Hadačová, Věra Turková, Daniela Přikrylová

Biologia plantarum 26:349-357, 1984 | DOI: 10.1007/BF02898571

Using biospecific chromatography on fetuin D-Glc-Separon H 1.000, lectins were isolated from the seeds of six representatives of the speciesPhaseolus vulgaris ssp.aborigineus of different geographical origin. The lectins of all the six representatives exhibit the agglutinating activity against rabbit erythrocytes (non-treated, tryp3in-treated and pronase-treated) as well as against human erythrocytes (irrespective of blood group) but of different quantity. Lectins isolated from 4 seed types showed mitogenic activity against lymphocytes of murine spleen, whereas in two seed types mitogenic activity was not proved. Isoelectric focusing...

Transport and regulative properties of phenylacetic acid

S. ProchÁzka, V. Borkovec

Biologia plantarum 26:358-363, 1984 | DOI: 10.1007/BF02898573

The mode of transport and regulative properties of phenylacetic acid (PAA) were studied in 11-cm long segments of pea epicotyls capable of growth. The transport of PAA m both the basipetal and aeropetal direction was limited to tissues situated immediately below and/or above the site of its application. PAA was able to promote the flow of14C-ABA in the acropetal direction. PAA-initiated thickening growth was probably the cause of this promotion.

Behaviour of nodulatedPisum sativum L. under short-term nitrate stress conditions

V. Škrdleta, Alena Gaudinová, Marie Němcová, Ludmila Lisá

Biologia plantarum 26:364, 1984 | DOI: 10.1007/BF02898575

Nitrate (20 mM) applied to the root medium of 28-day-old nodulated pea plants (Pisum sativum L., cv. Jupiter) immediately retarded nodule growth and inhibited root nodulation. Acetylene-reducing and H2-evolving nitrogenase activities were also significantly inhibited. The inhibitory effect of nitrate on nodule respiration was less pronounced while the respiration of roots was increased after the addition of nitrate. The levels of cytosol glutamine synthetase and nitrate reductase in nodule cytosol were permanently decreased from the 4th day after nitrate application. These results indicate that the inhibitory effect of high nitrate...

In vitro propagation of oak (Quereus robur L.) and linden (Tilia cordata Mill.)

V. Chalupa

Biologia plantarum 26:374-377, 1984 | DOI: 10.1007/BF02898577

Rapid multiplication of axillary shoots of oak and linden has been achieved on broad-leaved tree medium (BTM) and woody plant medium (WPM) containing low level of cytokinin (BAP 0.2-1.0 mg l-1). High rooting percentages (80-95%) were obtained on low salt, low sucrose media, containing low level of auxins. Rooted plants were transplanted into pots containing a mixture of peat and perlite. Most of the plants (90-95%) survived the transfer. After the hardening off period the new plants were planted in the field.

Proteins of aleurone grains isolated from cotyledons ofLupinus luteus L.

Ligia Konopska

Biologia plantarum 26:378, 1984 | DOI: 10.1007/BF02898578

Lupin aleurone grains from dry cotyledons contain about 87% of globulin including 57% of proteins solubilized by NaCl solution and 30% by NaOH extractions, and also about 12% of albumin. Activity of acid protease with the optimum at pH 3.6-3.8 was associated with albumin. Albumin was separated by gel filtration on Ultrogel AcA 22 into 3 fractions with mol. m. 179 000, 113 000, and below 100 000, respectively. The component with mol. m. 113 000 prevailed and comprised about 60% of albumin. Globulin was composed mainly of two major components with mol. m. above 364 000 and about 280 000. These correspond to α-and β-conglutin fractions, respectively.γ-conglutin...

Brief Communications

Effect of glycinebetaine on water use efficiency of wheat (Triticum aestivum L.)

H. Bergmann, H. Eckert

Biologia plantarum 26:384, 1984 | DOI: 10.1007/BF02898579

Long-term effects of glycinebetaine on water use efficiency (WUE), dry matter production and net photosynthetic rate have been studied. Applied glycinebetaine enhanced the WUE and the grain yield of wheat.