Book review

T. Gichner

Biologia plantarum 32:170, 1990 | DOI: 10.1007/BF02890866

Book review

Ingrid Tichá

Biologia plantarum 32:180, 1990 | DOI: 10.1007/BF02890868

Book review

T. Gichner

Biologia plantarum 32:188, 1990 | DOI: 10.1007/BF02890870

Book review

Frideta Seidlová

Biologia plantarum 32:197, 1990 | DOI: 10.1007/BF02890872

Book review

T. Gichner

Biologia plantarum 32:210, 1990 | DOI: 10.1007/BF02890875

Book review

Blanka Wichterlová

Biologia plantarum 32:222, 1990 | DOI: 10.1007/BF02890878

Book review

J. Tupý

Biologia plantarum 32:231, 1990 | DOI: 10.1007/BF02890881

Dark-induced changes in the content of phenolic acids in callus culture of alfalfa (Medicago sativa)

Milena Cvikrová, L. Meravý, Marie Hrubcová, J. Eder

Biologia plantarum 32:161, 1990 | DOI: 10.1007/BF02890865

The changes in the content of free and esterified phenolic acids extracted with methanol and the phenolic acids nonextractable with this solvent were studied during a long term cultivation (4 subcultures, 27 weeks total) of alfalfa callus in darkness and light. The total content of phenolic acids in the callus grown in darkness was lower as compared to their content in callus cultivated under 10/14 h light/darkness regime. It was decreasing during cultivation differently in the two treatments being in light-grown callus dependent on the length of cultivation and decreasing by each subcultivation in darkness. The portion of benzoic acid derivatives...

Molecular correlates of tissue browning and deterioration in Scots pine calli

Aija Lindfors, Hanna Kuusela, Anja Hohtola, Sirkka Kupila-Ahvenniemi

Biologia plantarum 32:171, 1990 | DOI: 10.1007/BF02890867

Callus cultures were initiated from the bud apices of 10-40-year-old Scots pines (Pinus sylvestris L.) at different seasons and maintained on modified MS medium without subculturing. Separate sets of experiments were used for analyses of carbohydrate content, ethylene production, amino acid composition, protein patterns andin vitro translation. In each case the change in the colour of the calli was recorded and the fresh mass of the samples measured. The onset of tissue browning was found to be associated with changes in protein pattern, amino acid content, ethylene production and the occurrence of sucrose and accumulation of starch.In...

In vitro induction of axillary potato microtubers and improvement of their quality

Z. Sladký, Ladislava Bartošová

Biologia plantarum 32:181, 1990 | DOI: 10.1007/BF02890869

Germinated tubers of selected cultivars Kera, Resy, Nicola and Oreb were made healthier by heat treatment. They were derived from germ explants on MS media with the addition of BAP 1 mg 1^-1 and IAA 0.2 mg 1^-1. After sufficient multiplication of stems, optimum conditions of the photoperiod were followed for the induction of axillary microtubers on stem segments in media with BAP 10 mg 1^-1 and 8% sucrose. The ability of tuberization is different: the early cvs. Kera and Resy induce earlier tubers at a long photoperiod and late cvs. Nicola and Oreb tuber rather at a short photoperiod. In suitable photoperiods the inhibitory...

Polyamine accumulation in aged wheat seeds

M. Cecilia Anguillesi, Isa Grilli, R. Tazziolo, C. Floris

Biologia plantarum 32:189, 1990 | DOI: 10.1007/BF02890871

The present work was conducted to evaluate the content of the main polyamines (Spm, Spd, Put) in a series of naturally aged durum-wheat seeds as well as the activities of the enzymes ODC and ADC involved in their biosynthesis. In dry seeds the content of polyamines, especially that of Spd, rose during ageing till 6 years and then declined sharply. However, an increase of PA content upon imbibition was observed only with the youngest seeds, while a decrease was found in the older ones.The activities of ODC and ADC differed in aged seeds, the ODC activity being constant and lower than the ADC in the course of seed ageing. The ADC increased till the...

Phaseolus coccineus L. Storage Proteins. Extraction and Characterization

R. Bernardi, Maria C. Lupi, M. Durante

Biologia plantarum 32:198, 1990 | DOI: 10.1007/BF02890873

Phaseolus coccineus storage globulins were extracted from mature cotyledons, purified and characterized. Three major proteins were separated. A component showing erythroagglutinating activity was thoroughly purified by thyroglobulin-Sepharose chromatography. The relative molecular masses of the three fractions are Mr = 330, 178, and 500 kDa as determined by polyacrylamide gel electrophoresis (PAGE). They correspond to the proteins found in other systems and classified as phytohaemagglutinin (PHA), vicilin and legumin, respectively. Electrophoretic analyses under denaturating conditions (SDS-PAGE) evidenced the major subunits for the three proteins....

Simple inexpensive laboratory equipment for cultivation of plants under various CO₂ concentrations

R. HÁK, L. NÁTR

Biologia plantarum 32:205-210, 1990 | DOI: 10.1007/BF02890874

A simple equipment was developed to cultivate young cereal plants under enhanced CO₂ concentration. Cultivation system permits growing of about 40 barley seedlings for about 2 to 3 weeks. The system consists of two identical growth chambers (volume about 30 dm³), gas conditioning circuit and measuring circuit with an infra-red CO₂ analyser. Capabilities of the whole equipment were tested by growing barley plants under 330 and 1000 cm³ (CO₂)m^-3 and in combination with high or low nitrate level.

Characterization of Plasma membrane of winter wheat (TritiCum aestivum L.) Seedlings and Changes in ATP hydrolysing activity under low temparature stress

K. K. Baruah, N. V. Alyoshina, N. B. Astakhova, T. I. Trunova

Biologia plantarum 32:211, 1990 | DOI: 10.1007/BF02890876

Plasma membranes were isolated from young seedlings of cold hardened and non-hardened winter wheat using aqueous polymer two phase system consisting of dextran-polyethyleneglycol. Electron microscopic analysis and activities of various marker enzymes revealed that the upper phase was enriched in plasma membrane and free from contamination by other membranes. The fraction thus obtained possessed Mg ATP hydrolysing activity with an optimum pH 6.5 and the activity was stimulated by K⁺. The activity was highly sensitive to orthovanadate, diethylstilbesterol, dicyclohexylcarbodimide, insensitive to azide and nitrate. It was suggested that the...

The effect of Aminooxyacetic acid on ethylene production induced by methyl jasmonate in tomatoes

M. Saniewski, J. Czapski

Biologia plantarum 32:218, 1990 | DOI: 10.1007/BF02890877

Aminooxyacetic acid (AOA), an inhibitor of ACC biosynthesis, applied together with methyl jasmonate (JA-Me) to mature green and light-green tomatoes cv. Venture greatly inhibited ethylene production stimulated by JA-Me, when analyzed in ripe and overripe stages. AOA applied alone did not affect ethylene production in the same conditions of treatment and analysis. It is suggested that after JA-Me treatment of tomatoes the turnover rate of ACC is higher (JA-Me stimulates EFE activity) in comparison to control tissues, and, consequently, AOA inhibited ethylene production stimulated by methyl jasmonate.

Inheritance of gliadin proteins controlled by 1D chromosome in F₂ hybrids oftriticum aestivum cultivars

Ts. Stoilova, G. Ganeva, B. Bochev, K. Petrolitcheva

Biologia plantarum 32:223-225, 1990 | DOI: 10.1007/BF02890879

The inheritance of the gliadin spectra in F₂ hybrids between cultivars hexaploid wheat Bezostaya 1 and Bisel has been investigated. The results from the electrophoretic examination and analysis of the gliadin spectra reveal that the components 1,2,3,11 and 15 from the Bezostaya 1 spectrum and 1,2, 3, 7,11 and 15 from the Bisel spectrum are controlled by allelic variants of one gliadin coding locus in 1D chromosome.

Effect of iso-osmotic levels of salt and peg-6000 on germination and early seedling growth of pea (Pisum sativum L.)

Maharaj Singh, B. B. Singh, P. C. Ram

Biologia plantarum 32:226-231, 1990 | DOI: 10.1007/BF02890880

Germination and seedling growth of pea (Pisum sativum L. cv. Rachna) was studied in salts and PEG-6000 solution having osmotic potentials -0.1, -0.2, -0.3, -0.4 and -0.5 MPa. At equivalent level of stress, NaCl proved more harmful to germination, seedling growth, vigour index, as well as initial mobilizing efficiency of food material from seed to the growing seedling, while PEG-6000 was more harmful to imbibition rate and mobilization efficiency in further days.

Effect of iso-osmotic levels of salts and PEG-6000 on saccharides, free proline and nitrogen content during early seedling growth of pea (Pisum sativum L.)

Maharaj Singh, B. B. Singh, P. C. Ram

Biologia plantarum 32:232, 1990 | DOI: 10.1007/BF02890882

Effects of iso-osmotic levels of salts (NaCl, CaCl₂, Na₂So₂) and PEG-6000 on saccharides, free proline and nitrogen contents were studied in cotyledons of pea. Saccharide (total, reducing andnon-reducing) and nitrogen contents decreased with increasing the salt and water stress as compared to control at all the stages of seedling growth. PEG-induced stress had more deleterious effect on total saccharide content and non-reducing saccharide (NRS) content, while salt stress was more harmful to reducing saccharide (RS) and nitrogen content. Free proline content of cotyledons increased with increasing the salt and...

Effect of water stress on seed germination and seedling growth inOryza sativa L.

M. Takaki

Biologia plantarum 32:238, 1990 | DOI: 10.1007/BF02890883

The effect of water stress induced by application of polyethylene glycol 6000 during seed germination and seedling growth ofOryza sativa L. cv. IAC 165 was analysed. The seed germination was inhibited by the decrease in the water potential of the medium, the inhibition being greater under white light than under continuous darkness. When the seedling was submitted to water stress (-0.51 MPa) white light inhibited growth of root, coleoptile-and leaf, while under no stress conditions white light caused increase in growth of root and leaf and only inhibition of coleoptile growth.