Biologia plantarum 64: 289-301, 2020 | DOI: 10.32615/bp.2019.093

Comparative analysis of nitrogen metabolism-related genes in two inbred maize lines with different low-nitrogen tolerance

M. YAN, J. LUO, L. LIANG, L. CHEN, Y.Y. CAO, Y.H. CHEN, X.Y. ZHU, C.M. YU*
Scientific Observing and Experimental Station of Maize in Plains Area of Southern Region,
Ministry of Agriculture and School of Life Sciences, Nantong University, Nantong 226019, Jiangsu, P.R. China

Maize is an important crop and its nitrogen use efficiency (NUE) has been an issue for plant scientists and breeders for many years. To meet the demand of genetic diversity in cultivating local elite maize cultivars, researchers need to isolate germplasms with a high NUE. In this study, 30 maize inbred lines were screened under hydroponic conditions, and three inbred lines that tolerate low nitrogen concentration were identified. A comparative analysis of genes involved in N uptake, transport, and assimilation between two lines with different tolerances revealed that the low-nitrogen-tolerant inbred line MBST exhibited higher expressions of nitrate and ammonium transporters, especially ZmNRT3.1B, than less tolerant B73. This suggests that the MBST line had a more efficient high-affinity transporter system. We also showed that low-nitrogen conditions down-regulated the expressions of master genes, ZmNLPs, which were negatively correlated with the expressions of the nitrate transporters ZmNRTs. This indicates the existence of regulators that antagonize the function of ZmNLPs. Some genes related to N assimilation and carbon metabolism were also more expressed in MBST. This research shows that the low-nitrogen-tolerant line, MBST, transported nitrate and ammonium more effectively than the B73 line. The NUE was also higher in MBST than in B73.

Keywords: gene expression, high-affinity transporter, nitrogen use efficiency, Zea mays.

Received: January 20, 2019; Revised: May 16, 2019; Accepted: May 19, 2019; Published online: April 3, 2020Show citation

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YAN, M., LUO, J., LIANG, L., CHEN, L., CAO, Y.Y., CHEN, Y.H., ZHU, X.Y., & YU, C.M. (2020). Comparative analysis of nitrogen metabolism-related genes in two inbred maize lines with different low-nitrogen tolerance. Biologia plantarum, 64, 289-301. doi: 10.32615/bp.2019.093.
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