Biologia plantarum 64: 725-735, 2020 | DOI: 10.32615/bp.2020.104

Analysis of ABC1 protein family members in Lepidium apetalum seeds and the expression of LaAbc1 in seedlings in response to abiotic stresses

Q.L. YANG1, Z.Y. CHEN1,2, H. LU3, H.T. XIE1, J.Y. LI1, Y. DU1, S.C. HAN2, H.P. ZHAO1,2, H.X. ZHAO1,*
1 College of Life Science, Xinjiang Normal University, Xinjiang Urumqi 830054, P.R. China
2 College of Life Sciences, Beijing Normal University, Beijing 100875, P.R. China
3 Dough academy, Hotan Teachers College, Xinjiang Hotan 848000, P.R. China

To study the biological function of activity of bcl complex (ABC1) proteins in Lepidium apetalum Willd., genes encoding ABC1 family proteins were identified from the seed transcriptome. The sequence most closely related to germination at a low temperature was selected and gene expressions in response to low temperature stress further studied. The results show that 21 ABC1 genes were expressed in seeds germinating at the low temperature: 4 genes were upregulated, 6 were downregulated, and 11 were not significantly different from controls. The results of fluorescence quantification of the low-temperature stress on the seedlings of 7-d-old L. apetalum showed that seven genes were up-regulated, six genes were down-regulated, and eight genes had no significant difference. Real-time quantitative PCR results show that under the low temperature stress, the expression of the LaAbc1-3 gene increased, but its expression decreased after some time. The expression of this gene increased again after removing the low temperature stress. The expression of LaAbc1-21 gene in L. apetalum seedlings showed a trend of decreasing first and then increasing. The LaAbc1-3 gene was insensitive to salt stress. Expression of the LaAbc1-21 gene was significantly up-regulated during the salt stress. Under osmotic stress, the expression of the LaAbc1-3 gene was down-regulated, and the expression was negatively correlated with polyethylene glycol (PEG-6000) concentration. Under the PEG-6000 treatment, the expression of the LaAbc1-21 gene was significantly up-regulated, and the expression was positively correlated with concentration. These results provide a basis for further analysis of the role of the ABC1 genes in the stress resistance of L. apetalum.

Keywords: LaAbc1-3 and LaAbc1-21 genes, low temperature, osmotic stress, salt stress.

Received: June 4, 2020; Revised: July 8, 2020; Accepted: July 13, 2020; Published online: October 29, 2020Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
YANG, Q.L., CHEN, Z.Y., LU, H., XIE, H.T., LI, J.Y., DU, Y., ... ZHAO, H.X. (2020). Analysis of ABC1 protein family members in Lepidium apetalum seeds and the expression of LaAbc1 in seedlings in response to abiotic stresses. Biologia plantarum, 64, 725-735. doi: 10.32615/bp.2020.104.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files

    Download fileYang6505_Suppl.pdf

    File size: 1.51 MB

    References

    1. Bousquet, I., Dujardin, G., Slonimski, P.P.: ABC1, a novel yeast nuclear gene has a dual function in mitochondria: it suppresses a cytochrome b mRNA translation defect and is essential for the electron transfer in the bc 1 complex. - EMBO J. 10: 2023-2031, 1991. Go to original source...
    2. Do, T.Q., Hsu, A.Y., Jonassen, T., Lee, P.T., Clarke, C.F.: A defect in coenzyme Q biosynthesis is responsible for the respiratory deficiency in Saccharomyces cerevisiae abc1 mutants. - J. biol. Chem. 276: 18161-18168, 2001. Go to original source...
    3. Ernster, L., Forsmark, A.P.: Ubiquinol: an endogenous antioxidant in aerobic organisms. - Clin. Invest. 71: S60-S65, 1993. Go to original source...
    4. Gao, Q., Yang, Z., Zhou, Y., Yin, Z., Qiu, J., Liang, G.: Characterization of an ABC1 kinase family gene osABC1-2 conferring enhanced tolerance to dark-induced stress in rice. - Gene 498: 155-163, 2012. Go to original source...
    5. Gao, Q.S., Yang, Z.F., Zhou Y., Zhang, D., Yan, C.H., Liang, G.H., Xu, C.W.: Cloning of a maize ABC1 gene ZmABC1-10 and its response to abiotic stresses such as cadmium. - J. Crops 12: 2073-2083, 2010.
    6. Gao, Q.S., Zhang, D., Xu, L., Xu, C.W.: Systematic identification of rice ABC1 gene family and its response to abiotic stress. - Rice Sci. 3: 14-24. 2011a. Go to original source...
    7. Gao, Q.S., Zhang, D., Xu, L., Xu, C.W.: Identification of rice ABC1 gene family and expression analysis under abiotic stress. - Chin. J. Rice Sci. 1: 1-10. 2011b. Go to original source...
    8. Hsieh, E.J., Dinoso, J.B., Clarke, C.F.: A trnatrp gene mediates the suppression of cbs2-223 previously attributed to abc1/coq8. - Biochem. biophys. Res. Commun. 317: 648-653, 2004. Go to original source...
    9. Jasinski, M., Sudre, D., Schansker, G., Schellenberg, M., Constant, S., Martinoia, E.: AtOSA1, a member of the ABC1-like family, as a new factor in cadmium and oxidative stress response. - Plant Physiol. 147: 719-731, 2008. Go to original source...
    10. Leonard, C.J., Aravind, L., Koonin, E.V.: Novel families of putative protein kinases in bacteria and archaea: evolution of the "eukaryotic" protein kinase superfamily. - Genome Res. 8: 1038-1047, 1998. Go to original source...
    11. Li, L.L., Zeng, W.J., Li, Y.H., Ge, F.W., Lu, H., Lei, W., Du, Y., Xie, H.T., Zhao, H.P., Zhao, H.X.: Analysis of GRAS transcription factor family and cloning of LaSCL18 gene in Lepidopsis uniquely and its cold-related study. - Mol. Plant Breed. 15: 3428-3437, 2017.
    12. Li, P.P., Zeng, W.J., Zhou, Q., Zhao, H.X., Li, Y.H., Ge, F.W., Zhu, C.Q., Zhao, J.J., Lu, H., Zhao, H.P.: Screening and expression analysis of temperature response proteins to remove the stagnation of low temperature germination of Lepidopsis uniflora seeds. - Mol. Plant Breed. 14: 722-729, 2016.
    13. Macinga., D.R., Cook, G.M., Poole, R.K., Rather, P.N.: Identification and characterization of aarf, a locus required for production of ubiquinone in Providencia stuartii and Escherichia coli and for expression of 2'-N-acetyltransferase in P. stuartii. - J. Bacteriol. 180: 128-135, 1998. Go to original source...
    14. Mao, Z.M., Zhang, X.M.: Flora outline of early spring short-lived plants in northern Xinjiang. - Arid Zone Res. 11: 1-26, 1994.
    15. Meng, J., Li, Q., Li, G.: Study on the physiological characteristics of seed germination of two species of Lepidopsis. - Biotechnology 18: 32-35, 2008.
    16. Mollet, J., Delahodde, A., Serre, V., Chretien, D., Schlemmer, D., Lombes, A., Boddaert, N., Desguerre, I., De Lonlay, P., De Baulny, H.O., Munnich, A., Rötig, A.: CABC1 gene mutations cause ubiquinone deficiency with cerebellar ataxia and seizures. - Amer. J. Human Genet. 82: 623-630, 2008. Go to original source...
    17. Smita, S., Katiyar, A., Lenka, S.K., Dalal, M., Kumar, A., Mahtha, S.K.: Gene network modules associated with abiotic stress response in tolerant rice genotypes identified by transcriptome meta-analysis. - Funct. integr. Genomics 20: 29-49, 2020. Go to original source...
    18. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S.: MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. - Mol. Biol. Evol. 28: 2731- 2739, 2011. Go to original source...
    19. Tauche, A., Krause, B.U., Rodel, G.: Ubiquinone biosynthesis in Saccharomyces cerevisiae: the molecular organization of o-methylase coq3p depends on abc1p/coq8p. - FEMS Yeast Res. 8: 1263-1275, 2013.
    20. Thompson, J.D., Plewniak, F., Poch, O.: A comprehensive comparison of multiple sequence alignment programs. - Nucl. Acids Res. 27: 2682-2690, 1999. Go to original source...
    21. Trumpower, B.L.: New concepts on the role of ubiquinone in the mitochondrial respiratory chain. - J. Bioenerg. 13: 1-24, 1981. Go to original source...
    22. Trumpower, B.L.: Cytochrome bc1 complexes of microorganisms. - Microbiol. Rev. 54: 101-129, 1990. Go to original source...
    23. Villalba, J.M., Navas, P.: Plasma membrane redox system in the control of stress-induced apoptosis. - Antioxid. Redox Signaling 2: 213-230, 2000. Go to original source...
    24. Wang, C.X., Jing, R.L., Mao, X.G., Chang, X.P., Li, A.: TaABC1, a member of the activity of bc1 complex protein kinase family from common wheat, confers enhanced tolerance to abiotic stresses in Arabidopsis. - J. exp. Bot. 62: 1299-1311, 2011. Go to original source...
    25. Wang, C.X., Jing, R.L., Mao, X.G., Pang, X.B., Liu, H.M., Chang, X.P.: Cloning and expression analysis of wheat TaABC1L. - J. Crops. 33: 878-884, 2004.
    26. Yang, N., Zhao, H.P., Ge, F.W., Li, Y.H., Zeng, W.J., Zhao, H.X.: Physiological response of germination of two species of Lonicerae to low temperature stress. - Arid Zone Res. 32: 760-765, 2015.
    27. Yang, Q.L., Lu, H., Zhou, Q., Xie, H.T., Li, J.Y., Du, Y., Chen, Z.Y., Han, S.C., Zhao, H.P., Zhao, H.X.: Analysis of LEA protein family members in Lepidium apetalum seeds and the expression of LaLEA1 in seedlings in response to abiotic stresses. - Biol. Plant. 64: 211-219, 2020. Go to original source...
    28. Yang, S.G., Li, T., Liu, M., Gao, S.J., Yang, S.Y., Li, L.: Phylogenetic, structure and expression analysis of ABC1ps gene family in rice. - Biol. Plant. 56: 667-674, 2012a. Go to original source...
    29. Yang, S.G., Zeng, X.Q., Li, T., Liu, M., Zhang, S.H., Gao, S.J., Wang, Y.Q., Peng, C.L., Li, L., Yang, C.W.: Atacdo1, an ABC1-like kinase gene, is involved in chlorophyll degradation and the response to photooxidative stress in Arabidopsis. - J. exp. Bot. 63: 3959-3973, 2012b. Go to original source...
    30. Yang, S., Zhang, Q., Li, T., Du, J., Yang, S., Yang, C.: AtSIA1, an ABC1-like kinase, regulates salt response in Arabidopsis. - Biológia 67: 1107-1111, 2012c. Go to original source...
    31. Yuan, L.L., Wang, Y.R., Zeng, W.J., Xie, H.T., Du, Y., Lu, H., Zhao, H.X.: Response of bHLH transcription factor gene family and laICE1 expression in seedlings of uninhabited vegetable to cold stress. - Acta bot. boreali-occident. sin. 38: 26-34, 2018.
    32. Yuan, Y.Y., Liang, Y., Yuan, S.X., Xu, L.F., Li, Y.N., Younes, P.H., Liu, C., Ming, J.: Cloning of the lily ABC1 protein kinase gene and its expression patterns under abiotic stresses in Lilium regale. - Acta hort. sin. 41: 2246-2454, 2014.
    33. Young, B.M., Hua, C.L., Jungeun, L., Hyun, P., Andosung, L., Taek, K.W., Hyoungseok, L.: Identification of rice genes associated with enhanced cold tolerance by comparative transcriptome analysis with two transgenic rice plants overexpressing dacbf4 or dacbf7, isolated from antarctic flowering plant Deschampsia antarctica. - Front. Plant Sci. 9: 601-610, 2018.
    34. Zhao, H.X., Li, Q., Jin, Z.: Tolerance to low temperature during seed germination of short-lived plants. - Plant Diversity Resour. 32: 448-454, 2010.
    35. Zhao, H.X., Zhao, Q.Q., Lu, H., Zhu, Y.L., Yu, L.J., Qian, H.B., Du, Y., Xie, H.T., Zhao, W.J., Zeng, W.J.: Response of DREB transcription factor family and LaDREB expression to cold stress in seedlings of Lepidopsis uniquely. - Mol. Plant Breed. 16: 42-48, 2018.
    36. Zhou, Q., Zhao, H.X., Li, P.P., Zeng, W.J., Li, Y.H., Ge, F.W., Zhao, J.J., Zhao, H.P.: High-throughput sequencing and analysis of the transcriptome of the seeds of Lepidopsis uniflora. - China Biotechnol. 36: 38-46, 2016.

    Return to the content