Biologia plantarum 64: 807-813, 2020 | DOI: 10.32615/bp.2020.151

Virus induced PhFTRv gene silencing results in yellow-green leaves and reduced cold tolerance in petunia

L. SANG1,2,3, L. PENG2, Z. QIU2, F. LUO1, G. CHEN1, L. GAO2, Y. YU2, J. LIU1,2,3,*
1 College of Horticulture, South China Agricultural University, Guangzhou 510642, P.R. China
2 College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
3 Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou, 510642, P.R. China

Ferredoxin-thioredoxin reductase (FTR) is an iron-sulfur protein that supplies electrons from photochemically reduced ferredoxin (Fd) to thioredoxin (Trx) in the ferredoxin/thioredoxin system in chloroplasts. The FTR is a heterodimer with a variable subunit (FTRv) and a catalytic subunit (FTRc). The function of FTRv is not well known. In petunia (Petunia hybrida), FTRv is a single-copy gene, which is named PhFTRv. In this study, the spatio-temporal expression of PhFTRv in petunia was analyzed, and PhFTRv transcription was found to be high in leaves and stems. A tobacco rattle virus gene silencing was used in this study. Virus induced gene silencing-mediated PhFTRv silencing resulted in large yellow-green leaves, delayed flowering, and reduced cold tolerance in petunia plants.

Keywords: flowering, chloroplasts, chlorophyll, leaf development, Petunia hybrida, photosynthesis, temperature stress.

Received: June 28, 2020; Revised: September 27, 2020; Accepted: October 12, 2020; Published online: December 15, 2020Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
SANG, L., PENG, L., QIU, Z., LUO, F., CHEN, G., GAO, L., YU, Y., & LIU, J. (2020). Virus induced PhFTRv gene silencing results in yellow-green leaves and reduced cold tolerance in petunia. Biologia plantarum, 64, 807-813. doi: 10.32615/bp.2020.151.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files

    Download file6523_Sang_Suppl.pdf

    File size: 320.09 kB

    References

    1. Balsera, M., Uberegui, E., Susanti, D., Schmitz, R.A., Mukhopadhyay, B., Schurmann, P., Buchanan, B.B.: Ferredoxin: thioredoxin reductase (FTR) links the regulation of oxygenic photosynthesis to deeply rooted bacteria. - Planta 237: 619-635, 2013. Go to original source...
    2. Belin, C., Bashandy, T., Cela, J., Delorme-Hinoux, V., Riondet, C., Reichheld, J.P.: A comprehensive study of thiol reduction gene expression under stress conditions in Arabidopsis thaliana. - Plant Cell Environ. 38: 299-314, 2015. Go to original source...
    3. Buchanan, B.B.: The path to thioredoxin and redox regulation in chloroplasts. - Annu. Rev. Plant Biol. 67: 1-24, 2016. Go to original source...
    4. Buchanan, B.B., Schurmann, P., Wolosiuk, R.A., Jacquot, J.P.: The ferredoxin/thioredoxin system: from discovery to molecular structures and beyond. - Photosynth. Res. 73: 215-222, 2002. Go to original source...
    5. Chae, H.B., Moon, J.C., Mi, R.S., Yong H.C., Jung, Y.J., Sun, Y.L., Nawkar, G.M.: Thioredoxin reductase type C (NTRC) orchestrates enhanced thermotolerance to Arabidopsis by its redox-dependent holdase chaperone function. - Mol. Plant. 6: 323-336, 2013. Go to original source...
    6. Chang, M.M., Li, A., Feissner, R., Ahmad, T.: RT-qPCR demonstrates light-dependent AtRBCS1A and AtRBCS3B mRNA expressions in Arabidopsis thaliana leaves. - Biochem. Mol. Biol. Edu. 44: 405-411, 2016.
    7. Chen, G., Liu, H., Wei, Q., Zhao, H., Liu, J., Yu, Y.: The acyl-activating enzyme PhAAE13 is an alternative enzymatic source of precursors for anthocyanin biosynthesis in petunia flowers. - J. exp. Bot. 68: 457-467, 2017.
    8. Dai, S., Schwendtmayer, C., Schuermann, P., Ramaswamy, S., Eklund, H.: Redox signaling in chloroplasts: cleavage of disulfides by an iron-sulfur cluster. - Science 287: 655-658, 2000. Go to original source...
    9. Dietz, K., Pfannschmidt, T.: Novel regulators in photosynthetic redox control of plant metabolism and gene expression. - Plant Physiol. 155: 1477-1485, 2011. Go to original source...
    10. Geigenberger, P., Fernie, A.: Metabolic control of redox and redox control of metabolism in plants. - Antioxid. Redox Signal. 21: 1389-1421, 2014. Go to original source...
    11. Hashida, S., Miyagi, A., Nishiyama, M., Yoshida, K., Hisabori, T., Kawai-Yamada, M.: Ferredoxin/thioredoxin system plays an important role in the chloroplastic NADP status of Arabidopsis. - Plant J. 95: 947-960, 2018. Go to original source...
    12. He, B., Gu, M., Wang, X., He, X.: The effects of lead on photosynthetic performance of waxberry seedlings (Myrica rubra). - Photosynthetica 56: 1147-1153, 2018. Go to original source...
    13. Keryer, E., Collin, V., Lavergne, D., Lemaire, S., Issakidis-Bourguet, E.: Characterization of Arabidopsis mutants for the variable subunit of ferredoxin:thioredoxin reductase. - Photosynth. Res. 79: 265-274, 2004. Go to original source...
    14. Lichtenthaler, H.K.: Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. - Method Enzymol. 148C: 350-382, 1987. Go to original source...
    15. Lim, C.J., Kim, W.B., Lee, B., Lee, H.Y., Kwon, T., Park, J.M., Kwon, S.: Silencing of SlFTR-c, the catalytic subunit of ferredoxin: thioredoxin reductase, induces pathogenesis-related genes and pathogen resistance in tomato plants. - Biochem. biophys. Res. Commun. 399: 750-754, 2010. Go to original source...
    16. Liu, J., Chang, X., Ding, B., Zhong, S., Peng, L., Wei, Q., Meng, J., Yu, Y.: PhDHS is involved in chloroplast development in Petunia. - Front. Plant Sci. 10: 284, 2019. Go to original source...
    17. Liu, J., Li, J., Wang, H., Fu, Z., Liu, J., Yu, Y.: Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments. - J. exp. Bot. 62: 825-840, 2011. Go to original source...
    18. Liu, Y., Schiff, M., Dinesh-Kumar, S.P.: Virus-induced gene silencing in tomato. - Plant J. 31: 777-786, 2002. Go to original source...
    19. Livak, K.J., Schmittgen, T.D.: Analysis of relative gene expression data using real-time quantitative PCR and the 2-DELTADELTACT method. - Methods 25: 402-408, 2001. Go to original source...
    20. Luo, T., Fan, T., Liu, Y., Rothbart, M., Yu, J., Zhou, S., Grimm, B., Luo M.: Thioredoxin redox regulates ATPase activity of magnesium chelatase CHLI subunit and modulates redox-mediated signaling in tetrapyrrole biosynthesis and homeostasis of reactive oxygen species in pea plants. - Plant Physiol. 159: 118-130, 2012. Go to original source...
    21. Nikkanen, L., Toivola, J., Rintamaki, E.: Crosstalk between chloroplast thioredoxin systems in regulation of photosynthesis. - Plant Cell Environ. 39: 1691-1705, 2016. Go to original source...
    22. Sakamoto, W., Zaltsman, A., Adam, Z., Takahashi, Y.: Coordinated regulation and complex formation of Yellow Variegated1 and Yellow Variegated2, chloroplastic FtsH metalloproteases involved in the repair cycle of photosystem II in Arabidopsis thylakoid membranes. - Plant Cell 15: 2843-2855, 2003. Go to original source...
    23. Schurmann, P., Buchanan, B.B.: The ferredoxin/thioredoxin system of oxygenic photosynthesis. - Antioxid. Redox Signal. 10: 1235-1274, 2008. Go to original source...
    24. Schurmann, P., Jacquot, J.P.: Plant thioredoxin systems revisited. - Annu. Rev. Plant Physiol. 51: 371-400, 2000. Go to original source...
    25. Serrato, A.J., Pérez-Ruiz, J.M., Spínola, M.C., Cejudo, F.J.: A novel NADPH thioredoxin reductase, localized in the chloroplast, which deficiency causes hypersensitivity to abiotic stress in Arabidopsis thaliana. - J. biol. Chem. 279: 43821-43827, 2004. Go to original source...
    26. Wang, P., Liu, J., Liu, B., Da, Q., Feng, D., Su, J., Zhang, Y., Wang, J., Wang, H.: Ferredoxin: thioredoxin reductase is required for proper chloroplast development and is involved in the regulation of plastid gene expression in Arabidopsis thaliana. - Mol. Plants 7: 1586-1590, 2014. Go to original source...
    27. Yoshida, K., Hisabori, T.: Distinct electron transfer from ferredoxin-thioredoxin reductase to multiple thioredoxin isoforms in chloroplasts. - Biochem. J. 474: 1347-1360, 2017. Go to original source...
    28. Yoshida, K., Noguchi, K., Motohashi, K., Hisabori, T.: Systematic exploration of thioredoxin target proteins in plant mitochondria. - Plant Cell Physiol. 54: 875-892, 2013. Go to original source...

    Return to the content