Biologia plantarum 45:321-326, 2002 | DOI: 10.1023/A:1016249113274

The Expression of a Cytosolic Cyclophilin Promoter from Periwinkle in Transgenic Tobacco Plants

A.-M. Droual1, J. Creche2, F. Andreu2, J.-C. Chenieux2, M. Rideau2, S. Hamdi3
1 Cropdesign NV, Zwijnaarde, Belgium
2 Faculty of Pharmacy, EA2106, Tours, France
3 Faculty of Sciences, UMR 619, BCMDP, Talence, France

The cloning of a 465 bp fragment from the 5'-flanking region of the gene encoding a cytosolic cyclophilin from periwinkle was achieved through inverse polymerase chain reaction. The DNA fragment was fused to a gusA-intron marker then introduced into tobacco by Agrobacterium tumefaciens-mediated transformation. Histochemical analysis of the transgenic shoot cultures demonstrated that the construct was able to drive GUS expression in stomata guard cells, but not in mesophyll cells when shoots were still attached to the callus from which they were initiated. In separated transgenic shoots and in seedlings, GUS was expressed in external and internal phloem and root hairs, respectively. GUS activity in transgenic tobacco seedlings was also investigated by fluorimetric assays. Treatments with NaCl or ABA decreased promoter activity whereas treatment with yeast extracts increased it.

Keywords: Catharanthus roseus; GUS expression; promoter analysis; stress wounding
Subjects: abscisic acid; Agrobacterium tumefaciens; auxins; Catharanthus roseus; cyclophilin promoter, cytosolic; cytokinins; β-glucuronidase; periwinkle; promoter analysis; stomata guard cells, β-glucuronidase expression in; stress, wounding; tobacco, cytosolic cyclophilin promoter; transgenic tobacco, expression of cytosolic cyclophilin promoter

Published: September 1, 2002Show citation

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Droual, A.-M., Creche, J., Andreu, F., Chenieux, J.-C., Rideau, M., & Hamdi, S. (2002). The Expression of a Cytosolic Cyclophilin Promoter from Periwinkle in Transgenic Tobacco Plants. Biologia plantarum, 45(3), 321-326. doi: 10.1023/A:1016249113274.
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