Biologia plantarum 46:1-6, 2003 | DOI: 10.1023/A:1022337411944

Cellular Damage to the Callus Cells of Potato Subjected to Freezing

M.A. Anjum1
1 Department of Horticulture, Univ. College of Agriculture, Bahauddin Zakariya University, Multan, Pakistan

Callus cells of potato (Solanum tuberosum L.) cv. Désirée were exposed to various subzero temperatures and examined for the freezing damage. In the cells subjected to -3 °C, plasma membranes appeared to be intact, while tonoplast seemed to be damaged and organelles to be swollen. After freezing at -6 °C, the damage became severe and plasma membranes were ruptured. After exposure to -10 °C, the damage was so severe that the cell organelles could not be recognised and cytoplasm became fragmented.

Keywords: cell organelles; cytoplasm; electron microscopy; freezing damage; plasma membranes; Solanum tuberosum
Subjects: callus cells damage, freezing; cell organelles, freezing; chilling stress; in vitro culture, callus, cells damage, freezing; plasma membrane, freezing; potato, cell damage after freezing; Solanum tuberosum; temperature, low, cell damage

Prepublished online: March 1, 2003; Published: July 1, 2003Show citation

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Anjum, M.A. (2003). Cellular Damage to the Callus Cells of Potato Subjected to Freezing. Biologia plantarum, 46(1), 1-6. doi: 10.1023/A:1022337411944.
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