Biologia plantarum 49:201-208, 2005 | DOI: 10.1007/s10535-005-1208-x

Oxidations of various substrates and effects of the inhibitors on purified mitochondria isolated from Kalanchoë pinnata

H. T. K. Hong1, A. Nose1, S. Agarie1
1 Faculty of Agriculture, Saga University, 1 Honjo-machi, Saga, Japan

Kalanchoë pinnata mitochondria readily oxidized succinate, malate, NADH, and NADPH at high rates and coupling. The highest respiration rates usually were observed in the presence of succinate. The high rate of malate oxidation was observed at pH 6.8 with thiamine pyrophosphate where both malic enzyme (ME) and pyruvate dehydrogenase were activated. In CAM phase III of K. pinnata mitochondria, both ME and malate dehydrogenase (MDH) simultaneously contributed to metabolism of malate. However, ME played a main function: malate was oxidized via ME to produce pyruvate and CO2 rather than via MDH to produce oxalacetate (OAA). Cooperative oxidation of two or three substrates was accompanied with the dramatic increase in the total respiration rates. Our results showed that the alternative (Alt) pathway was more active in malate oxidation at pH 6.8 with CoA and NAD+ where ME operated and was stimulated, indicating that both ME and Alt pathway were related to malate decarboxylation during the light. In K. pinnata mitochondria, NADH and NADPH oxidations were more sensitive with KCN than that with succinate and malate oxidations, suggesting that these oxidations were engaged to cytochrome pathway rather than to Alt pathway and these capacities would be desirable to supply enough energy for cytosol pyruvate orthophosphate dikinase activity.

Keywords: alternative pathway; CAM; cytochrome pathway; malate dehydrogenase; malic enzyme; oxalacetate
Subjects: abscisic acid (ABA); Crassulacean acid metabolism; cytochrome pathway; dehydration, rhizome ultrastructure, abscisic acid; desiccation tolerance; ferns, rhizome, dehydration and rewetting; Kalanchoë pinnata; malate dehydrogenase; malic enzyme; mitochondria; oxalacetate; pyruvate dehydrogenase; rhizome parenchyma; thiamine pyrophosphate

Received: April 9, 2004; Accepted: August 25, 2004; Published: June 1, 2005Show citation

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Hong, H.T.K., Nose, A., & Agarie, S. (2005). Oxidations of various substrates and effects of the inhibitors on purified mitochondria isolated from Kalanchoë pinnata. Biologia plantarum, 49(2), 201-208. doi: 10.1007/s10535-005-1208-x.
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