Biologia plantarum 50:74-80, 2006 | DOI: 10.1007/s10535-005-0077-7

Water transport in maize roots under the influence of mercuric chloride and water stress: a role of water channels

I. F. Ionenko1,*, A. V. Anisimov1, F. G. Karimova1
1 Institute of Biochemistry and Biophysics, Russian Academy of Sciences, Kazan, Russia

The influence of inhibitor of water channels, HgCl2, on water diffusion in maize (Zea mays L.) seedling roots was investigated with the pulsed nuclear magnetic resonance (NMR) method. Blocking of water channels decreased the water permeability of cell membranes by 1.5 - 2 times. This effect of HgCl2 was exhibited only in the roots of seedlings grown in a nutrient solution containing Ca2+ and was reversed with Hg-scavenging agent β-mercaptoethanol. Subsequent incubation of Ca2+-deficient roots in the nutrient solution with Ca2+ recovered the sensitivity to HgCl2. The water stress decreased water diffusion rates similarly to HgCl2 and the effects of water stress and HgCl2 were not additive. The obtained data demonstrate the possibilities of the pulsed NMR method for study of the transmembrane water exchange in vivo in connection with water channel functioning.

Keywords: diffusion rate; nuclear magnetic resonance; transmembrane transfer; Zea mays
Subjects: aquaporins; calcium; chalcone synthase; heavy metals; mercury chloride; root, rooting; transmembrane transfer; water channels, aquaporins; water stress; Zea mays

Received: January 20, 2004; Accepted: March 24, 2005; Published: March 1, 2006Show citation

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Ionenko, I.F., Anisimov, A.V., & Karimova, F.G. (2006). Water transport in maize roots under the influence of mercuric chloride and water stress: a role of water channels. Biologia plantarum, 50(1), 74-80. doi: 10.1007/s10535-005-0077-7.
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    References

    1. Abraham, A.: The Principles of Nuclear Magnetism.-Oxford University Press, London 1961.
    2. Anisimov, A.V., Evarestov, A.S., Ionenko, I.F., Gusev, N.A.: [Pulsed NMR method for estimation of intercellular water transport on symplasm.]-Dokl. Akad. Nauk SSSR 271: 1246-1249, 1983. [In Russ.]
    3. Anisimov, A.V., Ratkovic, S.: Transport Vody v Rasteniyakh: Issledovanie Impul'snym Metodom YaMR. [Water Transport in Plants: Determination by pulsed NMR.]-Nauka, Moskva 1992. [In Russ.]
    4. Anisimov, A.V., Sorokina, N.Yu., Dautova, N.R.: Water diffusion in biological porous systems: a NMR approach.-Magn. Resonance Imaging 16: 565-568, 1998. Go to original source...
    5. Azaizeh, H., Gunse, B., Steudle, E.: Effects of NaCl and CaCl2 on water transport across root cells of maize (Zea mays L.) seedlings.-Plant Physiol. 99: 886-894, 1992. Go to original source...
    6. Baiges, I., Schaffner, A.R., Affenzeller, M.J., Mas, A.: Plant aquaporins.-Physiol. Plant. 115: 175-182, 2002. Go to original source...
    7. Barrieu, F., Chaumont, F., Chrispeels, M.J.: High expression of the tonoplast aquaporin ZmTIP1 in epidermal and conducting tissues of maize.-Plant Physiol. 117: 1153-1163, 1998. Go to original source...
    8. Barrowclough, D.E., Peterson, C.A., Steudle, E.: Radial hydraulic conductivity along developing onion roots.-J. exp. Bot. 51: 547-557, 2000. Go to original source...
    9. Carvajal, M., Cooke, D.T., Clarkson, D.T.: Responses of wheat plants to nutrient deprivation may involve the regulation of water-channel function.-Planta 199: 372-381, 1996. Go to original source...
    10. Carvajal, M., Martinez, V., Alcaraz, C.F.: Physiological function of water channels is affected by salinity in roots of paprika pepper.-Physiol. Plant. 105: 95-101, 1999. Go to original source...
    11. Chaumont, F., Barrieu, F., Herman, E.M., Chrispeels, M.J.: Characterization of a maize tonoplast aquaporin expressed in zones of cell division and elongation.-Plant Physiol. 117: 1143-1152, 1998. Go to original source...
    12. Chaumont, F., Barrieu, F., Wojcik, E., Chrispeels, M.J., Jung, R.: Aquaporins constitute a large and highly divergent protein family in maize.-Plant Physiol. 125: 1206-1215, 2001. Go to original source...
    13. Chrispeels, M.J., Maurel, C.: Aquaporins: the molecular basis of facilitated water movement through living plant cells.-Plant Physiol. 105: 9-15, 1994. Go to original source...
    14. Clarkson, D.T., Carvajal, M., Henzler, T., Waterhouse, R.N., Smyth, A.J., Cooke, D.T., Steudle, E.: Root hydraulic conductance: diurnal aquaporin expression and the effects of nutrient stress.-J. exp. Bot. 51: 61-70, 2000. Go to original source...
    15. Cooper, R.L., Chang, D.B., Young, A.C., Martin, C.J., Ancker-Johnson, B.: Restricted diffusion in biological systems.-Biophys. J. 14: 161-177, 1974. Go to original source...
    16. Crick, F.: Diffusion in embryogenesis.-Nature 225: 420-422, 1970. Go to original source...
    17. Farrar, T.C., Becker, E.D.: Pulse and Fourier Transform NMR.-Academic Press, New York 1971. Go to original source...
    18. Frensch, J., Hsiao, T.S.: Transient responses of cell turgor and growth of maize roots as affected by changes in water potential.-Plant Physiol. 104: 247-254, 1994. Go to original source...
    19. Guerrero, F.D., Jones, J.T., Mullet, J.E.: Turgor-responsive gene transcription and RNA levels increase rapidly when pea shoots are wilted. Sequence and expression of three inducible genes.-Plant mol. Biol. 15: 11-26, 1990. Go to original source...
    20. Henzler, T., Steudle, E.: Reversible closing of water channels in Chara internodes provides evidence for a composite transport model of the plasma membrane.-J. exp. Bot. 46: 199-209, 1995. Go to original source...
    21. Hukin, D., Doering-Saad, C., Thomas, C.R., Pritchard, J.: Sensitivity of cell hydraulic conductivity to mercury is coincident with symplasmic isolation and expression of plasmalemma aquaporin genes in growing maize roots.-Planta 215: 1047-1056, 2002.
    22. Ionenko, I.F., Anisimov, A.V.: Effect of water deficit and membrane destruction on water diffusion in the tissues of maize seedlings.-Biol. Plant. 44: 247-252, 2001. Go to original source...
    23. Javot, H., Maurel, C.: The role of aquaporins in root water uptake.-Ann. Bot. 90: 301-313, 2002. Go to original source...
    24. Johansson, I., Karlsson, M., Shukla, V.K., Chrispeels, M.J., Larsson, C., Kjellbom, P.: Water transport activity of plasma membrane aquaporin PM28A is regulated by phosphorylation.-Plant Cell 10: 451-459, 1998. Go to original source...
    25. Johansson, I., Larsson, C., Ek, B., Kjellbom, P.: The major integral proteins of spinach leaf plasma membranes are putative aquaporins and are phosphorylated in response to Ca2+ and apoplastic water potential.-Plant Cell 8: 1181-1191, 1996. Go to original source...
    26. Kaldenhoff, R., Grote, K., Zhu, J.-J., Zimmermann, U.: Significance of plasmalemma aquaporins for water-transport in Arabidopsis thaliana.-Plant J. 14: 121-128, 1998. Go to original source...
    27. Kjellbom, P., Larsson, C., Johansson, I., Karisson, M., Johanson, U.: Aquaporins and water homeostasis in plants.-Trends Plant Sci. 4: 308-314, 1999. Go to original source...
    28. Krishnan, P., Joshi, D.K., Maheswari, M., Nagarajan, S., Moharir, A.V.: Characterization of soybean and wheat seeds by nuclear magnetic resonance spectroscopy.-Biol. Plant. 48: 117-120, 2004. Go to original source...
    29. Kuzmanoff, K.M., Evans, M.L.: Kinetics of adaptation to osmotic stress in lentil (Lens culinaris Med.) roots.-Plant Physiol. 68: 244-247, 1981. Go to original source...
    30. Maggio, A., Joly, R.J.: Effects of mercuric chloride on the hydraulic conductivity of tomato root systems.-Plant Physiol. 109: 331-335, 1995. Go to original source...
    31. Maurel, C., Kado, R.T., Guern, J., Chrispeels, M.J.: Phosphorylation regulates the water channel activity of the seed specific aquaporin α-TIP.-EMBO J. 14: 3028-3035, 1995. Go to original source...
    32. Murata, K., Mitsuoka, K., Hirai, T., Walz, T., Agre, P., Heymann, J.B., Engel, A., Fijlyoshi, Y.: Structural determinants of water permeation through aquaporin 1.-Nature 107: 599-605, 2000. Go to original source...
    33. Snaar, J.E.M., Van As, H.: Probing water compartment and membrane permeability in plant cells by proton NMR relaxation measurements.-Biophys. J. 63: 1654-1658, 1992. Go to original source...
    34. Stejskal, E.O., Tanner, J.E.: Spin-diffusion measurements: spin-echoes in the presence of a time-dependent field gradient.-J. chem. Phys. 42: 288-292, 1965. Go to original source...
    35. Steudle, E.: Water transport across plant tissue: role of water channels.-Biol. Cell 89: 259-273, 1997. Go to original source...
    36. Steudle, E.: Water uptake by roots: effect of water deficit.-J. exp. Bot. 51: 1531-1542, 2000. Go to original source...
    37. Tanner, J.E.: Use of the stimulated echo in NMR diffusion studies.-J. chem. Phys. 52: 2523-2526, 1970. Go to original source...
    38. Tazawa, M., Ohkuma, E., Shibasaka, M., Nakashima, S.: Mercurial-sensitive water transport in barley roots.-J. Plant Res. 110: 435-442, 1997. Go to original source...
    39. Tyerman, S.D., Bohnert, H.J., Maurel, S., Steudle, E., Smith, J.A.C.: Plant aquaporins: their molecular biology, biophysics and significance for plant water relations.-J. exp. Bot. 50: 1055-1071, 1999. Go to original source...
    40. Van As, H., Van Vliet, P.A., Schaafsma, T.J.: 1H spin-echo nuclear magnetic resonance in plant tissue. The effect of Mn (II) and water content in wheat leaves.-Biophys. J. 32: 1043-1051, 1980. Go to original source...
    41. Van Dusschoten, D., De Jager, P.A., Van As, H.: Extracting diffusion constants from echo-time-dependent PFG NMR data using relaxation-time information.-J. Magn. Resonance 116: 22-28, 1995. Go to original source...
    42. Van der Weerd, L., Claessens, M.M.A.E., Rutink, T., Vergeldt, F.J., Schaafsma, T.J., Van As, H.: Quantitative NMR microscopy of osmotic stress responses in maize and pearl millet.-J. exp. Bot. 52: 2333-2343, 2001. Go to original source...
    43. Wan, X., Zwiazek, J.J.: Mercuric chloride effects on root water transport in aspen seedlings.-Plant Physiol. 121: 939-946, 1999. Go to original source...
    44. Willmer, C.M., Padmasree, K., Raghavendra, A.S.: A novel method of measuring volume changes of mesophyll cell protoplasts and the effect of mercuric chloride on their osmotically-induced swelling.-J. exp. Bot. 50: 401-406, 1999. Go to original source...
    45. Yamada, S., Katsuhara, M., Kelly, W., Michalowski, C.B., Bohnert, H.J.: A family of transcripts encoding water channel proteins: tissue-specific expression in the common ice plant.-Plant Cell 7: 1129-1142, 1995. Go to original source...
    46. Yamaguchi-Shinozaki, K., Koizumi, M., Urao, S., Shinozaki, K.: Molecular cloning and characterization of 9 cDNAs for genes that are responsive to desiccation in Arabidopsis thaliana: sequence analysis of qne cDNA clone that encodes a putative transmembrane channel protein.-Plant Cell Physiol. 33: 217-224, 1992. Go to original source...
    47. Zhang, W.H., Tyerman, S.D.: Inhibition of water channels by HgCl2 in intact wheat root cells.-Plant Physiol. 120: 849-858, 1999. Go to original source...

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