Biologia plantarum 53:343-346, 2009 | DOI: 10.1007/s10535-009-0063-6

Genetic diversity assessment in greek Medicago truncatula genotypes using microsatellite markers

P. Akritidis1, P. V. Mylona2, A. S. Tsaftaris1,3, A. N. Polidoros1,*
1 Institute of Agrobiotechnology, CERTH, Thermi, Greece
2 Agricultural Research Center of Northern Greece, NAGREF, Thermi, Greece
3 Department of Genetics and Plant Breeding, AUTh, Thessaloniki, Greece

In this study we examined the genetic diversity and geographic scale of genotype distribution within the model legume species Medicago truncatula widely distributed in pasture and marginal agricultural lands in Greece and other Mediterranean countries. Thirty one Medicago truncatula and Medicago littorialis accessions were chosen on the basis of their geographical distributions and studied using 9 polymorphic simple sequence repeats (SSR) markers. The number of alleles per locus varied between 3 and 7. A total of 42 alleles were detected with a mean value of 4.66 alleles per locus. Geographic origin was not related with genotypic similarity among accessions. However, there were instances of close genetic relatedness between accessions from neighboring locations in a geographic compartment. In conclusion, the presented data revealed extensive M. truncatula genotype dispersal in Greece pointing to the significance of preserving local genetic resources in their natural environment.

Keywords: molecular markers; phylogenetic analysis; simple sequence repeats
Subjects: dendrogram; genetic diversity; Medicago littoralis; Medicago truncatula; microsatellite markers; simple sequence repeats (SSR)

Received: August 4, 2007; Accepted: February 20, 2008; Published: June 1, 2009Show citation

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Akritidis, P., Mylona, P.V., Tsaftaris, A.S., & Polidoros, A.N. (2009). Genetic diversity assessment in greek Medicago truncatula genotypes using microsatellite markers. Biologia plantarum, 53(2), 343-346. doi: 10.1007/s10535-009-0063-6.
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    References

    1. Baquerizo-Audiot, E., Desplanqe, A.B., Prosperi, J.M., Santoni, S.: Characterization of microsatellite loci in the diploid legume Medicago truncatula (barrel medic).-Mol. Ecol. Notes 1: 1-3, 2001. Go to original source...
    2. Bonnin, I., Ronfort, J., Wozniak, F., Olivieri, I.: Spatial effects and rare outcrossing events in Medicago truncatula (Fabaceae).-Mol. Ecol. 10: 1371-1383, 2001. Go to original source...
    3. Choi, H.K., Mun, J.H., Kim, D.J., Zhu, H., Baek, J.M., Mudge, J., Roe, B., Ellis, N., Doyle, J., Kiss, G.B., Young, N.D., Cook, D.R.: Estimating genome conservation between crop and model legume species.-Proc. nat. Acad. Sci. USA 101: 15289-15294, 2004. Go to original source...
    4. Dieringer, D., Schlotterer, C.: Microsatellite analyser (MSA): a platform independent analysis tool for large microsatellite data sets.-Mol. Ecol. Notes 3: 167-169, 2003. Go to original source...
    5. Dikshit, H.K., Jhang, T., Singh, N.K., Koundal, K.R., Bansal, K.C., Chandra, N., Tickoo, J.L., Sharma, T.R.: Genetic differentiation of Vigna species by RAPD, URP and SSR markers.-Biol. Plant. 51: 451-457, 2007. Go to original source...
    6. Ellwood, S.R., D'Souza, N.K., Kamphuis, L.G., Burgess, T.I., Nair, R.M., Oliver, R.P.: SSR analysis of the Medicago truncatula SARDI core collection reveals substantial diversity and unusual genotype dispersal throughout the Mediterranean basin.-Theor. appl. Genet.: 1-7, 2006. Go to original source...
    7. Jensen, J.L., Bohonak, A.J., Kelley, S.T.: Isolation by distance, web service.-BMC Genet. 6: 13, 2005. Go to original source...
    8. Joshi, P., Dhawan, V.: Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay.-Biol. Plant. 51: 22-26, 2007. Go to original source...
    9. Kumar, S., Tamura, K., Nei, M.: MEGA3: Integrated software for molecular evolutionary genetics. Analysis and sequence alignment.-Brief Bioinf. 5: 150-163, 2004. Go to original source...
    10. Mirali, N., El-Khouri, S., Rizq, F.: Genetic diversity and relationships in some Vicia species as determined by SDS-PAGE of seed proteins.-Biol. Plant. 53: 660-666, 2007. Go to original source...
    11. Mun, J., Kim, D.J., Choi, H.K., Gish, J., Debelle, F., Mudge, J., Denny, R., Endre, G., Saurat, O., Dudez, A.M., Kiss, G., Roe, B., Young, N., Cook, D.: Distribution of microsatellites in the genome of Medicago truncatula: a resource of genetic markers that integrate genetic and physical maps.-Genetics 172: 2541-2555, 2006. Go to original source...
    12. Ronfort, J., Bataillon, T., Santoni, S., Delalande, M., David, J.L., Prosperi, J.M.: Microsatellite diversity and broad scale geographic structure in a model legume: building a set of nested core collection for studying naturally occurring variation in Medicago truncatula.-BMC Plant Biol. 6: 28, 2006.
    13. Saitou, N., Nei, M.: The neighbor-joining method: a new method for reconstructing phylogenetic trees.-Mol. Biol. Evol. 4: 406-425, 1987.
    14. Sunnock, P.: Efficient genetic markers for population biology.-Trends Ecol. Evol. 12: 199-203, 2000. Go to original source...
    15. Takezaki, N., Nei, M.: Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA.-Genetics 144: 389-399, 1996.

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