Biologia plantarum 53:685-690, 2009 | DOI: 10.1007/s10535-009-0123-y

Comparison of drought-induced polypeptides and ion leakage in three tomato cultivars

R. Roy1, V. Agrawal1, S. C. Gupta2,*
1 Department of Botany, University of Delhi, Delhi, India
2 Dr. B.R. Ambedkar Centre for Biomedical Research, University of Delhi, Delhi, India

An attempt has been made to determine if drought-induced proteins could be used as a selection marker to differentiate between tolerant and sensitive cultivars. Three Indian tomato (Lycopersicon esculentum Mill.) cultivars (Pusa Ruby, Arka Vikas and Pusa Early Dwarf) were subjected to drought stress in vivo as well as in vitro and the pattern of polypeptide expression was determined using one-dimensional SDS-PAGE. In all the three cultivars, a new 29 kDa polypeptide accumulated in leaves, in response to gradual drought stress and its accumulation was fastest in Pusa Ruby. Drought stress also resulted in an increase in ion leakage from leaf discs of all the three cultivars but the rate was lower in Pusa Ruby than in other two. Therefore, it was concluded that Pusa Ruby is most tolerant to drought stress among the three tomato cultivars investigated.

Keywords: drought tolerance; Lycopersicon esculentum; mannitol; SDS-PAGE; water stress
Subjects: electrolyte leakage; Lycopersicon esculentum; proteins; tomato; water stress

Received: November 17, 2007; Accepted: April 11, 2008; Published: December 1, 2009Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Roy, R., Agrawal, V., & Gupta, S.C. (2009). Comparison of drought-induced polypeptides and ion leakage in three tomato cultivars. Biologia plantarum, 53(4), 685-690. doi: 10.1007/s10535-009-0123-y.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Alarcon, J.J., Sanchez-Blanco, M.J., Bolarin, M.C., Torrecillas, A.: Growth and osmotic adjustment of two tomato cultivars during and after saline stress. - Plant Soil 166: 75-82, 1994. Go to original source...
    2. Alian, A., Altman, A., Heuer, B.: Genotypic difference in salinity and water stress tolerance of fresh market tomato cultivars. - Plant Sci 152: 59-65, 2000. Go to original source...
    3. Bajji, M., Kinet, J.M., Lutts, S.: The use of electrolyte leakage method for assessing cell membrane stability as a water stress tolerance test in durum wheat. - Plant Growth Regul. 36: 61-70, 2002. Go to original source...
    4. Bolarin, M.C., Fernandez, F.G., Cruz, V., Cuartero, J.: Salinity tolerance in four wild tomato species using vegetative yield-salinity response curves. - J. amer. Soc. hort. Sci. 116: 286-289, 1991. Go to original source...
    5. Bramlage, W.J., Leopold, A.C., Parrish, D.J.: Chilling stress to soybeans during imbibition. - Plant Physiol. 61: 525-529, 1978. Go to original source...
    6. Bray, E.A.: Drought-stress-induced polypeptide accumulation in tomato leaves. - Plant Cell Environ 13: 531-538, 1990. Go to original source...
    7. Bray, E.A.: Genes commonly regulated by water-deficit stress in Arabidopsis thaliana. - J. exp. Bot. 55: 2331-2341, 2004. Go to original source...
    8. Caro, M., Cruz, V., Cuartero, J., Estan, M.T., Bolarin, M.C.: Salinity tolerance of normal-fruited and cherry tomato cultivars. - Plant Soil 136: 249-255, 1994.
    9. Caruso, A., Morabito, D., Delmotte, F., Kahlem, G., Sabine, C.: Dehydrin induction during drought and osmotic stress in Populus. - Plant Physiol. Biochem. 40: 1033-1042, 2002. Go to original source...
    10. Cuartero, J., Yeo, A.P., Flowers, T.J.: Selection of donors for salt tolerance in tomato using physiological traits. - New Phytol. 12: 63-69, 1992. Go to original source...
    11. Guerrier, G.: Fluxes of Na+, K+ and Cl- and osmotic adjustment in Lycopersicon pimpinellifolium and L. esculentum during short- and long-term exposure to NaCl. - Physiol. Plant. 97: 583-591, 1996. Go to original source...
    12. Jacobsen, J.V., Shaw, D.C.: Heat-stable proteins and abscisic acid action in barley aleurone cells. - Plant Physiol. 91: 1520-1526, 1989. Go to original source...
    13. Kawasaki, S., Borchert, C., Deyholos, M., Wang, H., Brazille, S., Kawai, K., Galbraith, D., Bohnert, H.J.: Gene expression profiles during the initial phase of salt stress in rice. - Plant Cell 13: 889-905, 2001. Go to original source...
    14. Kosová, K., Vítámvás, P., Prášil, I.T.: The role of dehydrins in plant response to cold. - Biol. Plant. 51: 601-617, 2007. Go to original source...
    15. Misra, S., Bewely, J.D.: Desiccation of Phaseolus vulgaris seeds during and following germination and its effect upon the translatable mRNA population of seed axes. - J. exp. Bot. 176: 364-374, 1986. Go to original source...
    16. Pelah, D., Wang, W., Altman, A., Shoseyov, O., Bartels, D.: Differential accumulation of water stress-regulated proteins, sucrose synthase and soluble sugars in Populus species that differ in their water stress response. - Physiol. Plant. 99: 153-159, 1997. Go to original source...
    17. Perez-Alfoncea, F., Estan, M.T., Caro, M., Guerrie, G.: Osmotic adjustment in Lycopersicon esculentum and L. penellii under NaCl and polyethyleneglycol 6000 isoosmotic stresses. - Plant Physiol. 87: 493-498, 1993. Go to original source...
    18. Peterson, G.L.: A simplification of the protein assay method of Lowry et al. which is more generally applicable. - Anal. Biochem. 83: 346-356, 1977. Go to original source...
    19. Suprunova, T., Krugman, T., Fahima, T., Chen, G., Shams, I., Korol, A., Nevo, E.: Differential expression of dehydrin genes in wild barley, Hordeum spontaneum, associated with resistance to water deficit. - Plant Cell Environ. 27: 1297-1308, 2004. Go to original source...
    20. Torreciallas, A., Guillaume, C., Alarcon, J.J., Ruiz-Sanchez, M.G.: Water relations of two tomato species under water stress and recovery. - Plant Sci. 105: 169-176, 1995. Go to original source...
    21. Vu, C.V., Allen, L.H., Garrad, L.A.: Effects of supplemental UV-B radiation on primary photosynthesis carboxylating enzymes and soluble proteins in leaves of C3 and C4 crop plants. - Physiol Plant 55: 11-16, 1982. Go to original source...
    22. Wahid, A., Close, T.J.: Expression of dehydrins under heat stress and their relationship with water relations of sugarcane leaves. - Biol. Plant. 51: 104-109, 2007. Go to original source...
    23. Waister, P.D., Hudson, J.P.: Effect of soil moisture regimes on leaf deficit transpiration and yield of tomatoes. - J. hort. Sci. 45: 359-370, 1970. Go to original source...

    Return to the content