Biologia plantarum 53:745-749, 2009 | DOI: 10.1007/s10535-009-0136-6

Improved shoot regeneration system through leaf derived callus and nodule culture of Sansevieria cylindrica

A. Shahzad1,*, N. Ahmad1, M. A. Rather1, M. K. Husain1, M. Anis1,*
1 Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, India

Long-term culture establishment and efficient in vitro regeneration protocol for Sansevieria cylindrica Bojer ex Hook was developed using leaf derived callus and nodule culture. Profuse callus induction on leaf discs was achieved on Murashige and Skoog (MS) medium supplemented with 10 µM indole-3-butyric acid (IBA), while a high frequency of nodulation was induced on 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) containing media. Shoot regeneration ability from cultured tissues occurred at varying degrees on all media. Through callus culture a maximum of 17.6 ± 0.14 shoots per culture was formed on medium containing 5µM 6-benzyladenine (BA) and 2 µM α-naphthaleneacetic acid (NAA). Among nodule cultures, the 2,4-D generated nodules were more proliferative and regenerative as compared to 2,4,5-T induced nodules and a maximum of 25 ± 0.16 shoots per culture was produced on a medium containing 5 µM BA plus 1 µM NAA. The regenerated shoots were successfully rooted on a semi-solid half strength MS medium containing 5 µM IBA with an average root number 3.5 ± 0.18 and root length 6.5 ± 0.14 cm. The regenerative ability of callus tissues was steady upto one year, while the nodules retained the totipotency to regenerate on optimal medium even after 3 years of subculturing. The histological sections of nodules confirm the typical anatomy exhibiting the vascular elements in bundles with well demarcated cortex and epidermal covering.

Keywords: auxins; cytokinins; multiple shoots; plant regeneration
Subjects: auxins; cytokinins; in vitro culture, callus; in vitro culture, indirect regeneration; in vitro culture, rooting; Sansevieria cylindrica; thidiazuron

Received: November 5, 2007; Accepted: July 17, 2008; Published: December 1, 2009Show citation

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Shahzad, A., Ahmad, N., Rather, M.A., Husain, M.K., & Anis, M. (2009). Improved shoot regeneration system through leaf derived callus and nodule culture of Sansevieria cylindrica. Biologia plantarum, 53(4), 745-749. doi: 10.1007/s10535-009-0136-6.
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