Biologia plantarum 55:159-163, 2011 | DOI: 10.1007/s10535-011-0022-x

Effect of salt stress on gene expression of superoxide dismutases and copper chaperone in Arabidopsis thaliana

H. Attia1,*, N. Karray1, N. Msilini1, M. Lachaâl1
1 Physiologie et Biochimie de la Tolérance au Sel des Plantes, Faculté des Sciences de Tunis, Campus Universitaire, Tunis El Manar, Tunisia

Arabidopsis thaliana plants (wild type accessions Col and N1438) were grown in nutrient solution for 34 d with or without 50 mM NaCl. Salt stress inhibited plant growth rate more in Col than in N1438 and a decrease in K+, Ca2+ and nitrogen contents was observed in both accessions. NaCl diminished accumulation of malate, fumarate and citrate only in Col accession. To measure the effect of NaCl on transcript level of superoxide dismutase (SOD) isoforms and copper chaperone for SOD genes, a semi-quantitative polymerase chain reaction (RT-PCR) method was developed using cDNA normalized against the EF1a gene in parallel with quantitative real time RT-PCR (Q-PCR) technique. Both methods gave the same results. The abundance of transcripts of the three genes coding for Cu/Zn-SOD responded similarly to NaCl in both accessions: CSD1 gene was overexpressed, and CSD2 and CSD3 genes were repressed. However, the genes coding for Fe-SOD (FSD1), Mn-SOD (MSD1) and Cu-chaperone for SOD (CCS) responded to NaCl differently in Col and N1438: the former gene was overexpressed in Col and repressed in N1438, and the opposite behaviour was observed for the latter two genes.

Keywords: citrate; fumarate; intraspecific variability; malate; mineral nutrition; RT-PCR

Received: March 31, 2009; Accepted: October 21, 2009; Published: March 1, 2011Show citation

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Attia, H., Karray, N., Msilini, N., & Lachaâl, M. (2011). Effect of salt stress on gene expression of superoxide dismutases and copper chaperone in Arabidopsis thaliana. Biologia plantarum, 55(1), 159-163. doi: 10.1007/s10535-011-0022-x.
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    References

    1. Abdel-Ghany, S., Müller-Moulé, P., Niyogi, K.K., Pilon, M., Shikanai, T.: Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. - Plant Cell 17: 1233-1251, 2005. Go to original source...
    2. Allen, R.D., Webb, R.P., Schake, S.A.: Use of transgenic plants to study antioxidant defenses. - Free Radical. Biol. Med. 23: 473-479, 1997. Go to original source...
    3. Arnesano, F., Banci, L., Bertini, I., Martinelli, M., Furukawa, Y., O'Halloran, T.V.: The unusually stable quaternary structure of human Cu/Zn-superoxide dismutase 1 is controlled by both metal occupancy and disulfide status. - J. biol. Chem. 279: 47998-48003, 2004. Go to original source...
    4. Borsani, O., Cuartero, J., Fernandez, J.A., Valpuesta, V., Botella, M.A.: Identification of two loci in tomato reveals distinct mechanisms for salt tolerance. - Plant Cell 13: 873-887, 2001. Go to original source...
    5. Bray, E.A., Bailey-Serres, J., Weretilnyk, E.: Responses to abiotic stresses. - In: Gruissem, W., Buchannan, B., Jones, R. (ed.): Biochemistry and Molecular Biology of Plants. Pp. 1158-1203. American Society of Plant Physiologists, Rockville, 2000.
    6. Gay, A.P., Hauck, B.: Acclimation of Lolium temulentum to enhanced carbon dioxide concentration. - J. exp. Bot. 45: 1133-1141, 1994. Go to original source...
    7. Gomez, J.M., Hernandez, J.A., Jimenez, A., del Rio, L.A., Sevilla, F.: Differential response of antioxidative systems of chloroplasts and mitochondria to long term NaCl stress of pea plant. - Free. Radical. Res. 31(Suppl.): 11-18, 1999. Go to original source...
    8. Hernandez, J.A., Corpas, F.J., Gomez, M., Del Rio, L.A., Sevilla, F.: Salt-induced oxidative stress mediated by activated oxygen species en pea leaf mitochondria. - Physiol. Plant 89: 103-110, 1993. Go to original source...
    9. Hernandez, J.A., Olmos, E., Corpas, F.J., Sevilla, F., Del Rio, L.A.: Salt-induced oxidative stress in chloroplast of pea plants. - Plant Sci. 105: 151-167, 1995. Go to original source...
    10. Hu, Y., Fricke, W., Schmidhalter, U.: Salinity and the growth of non-halophytic grass leaves: the role of mineral nutrient distribution. - Funct. Plant Biol. 32: 973-985, 2005. Go to original source...
    11. Kliebenstein, D.J., Monde, R.A., Last, R.L.: Superoxide dismutase in Arabidopsis: an electic enzyme family with disparate regulation and protein localization. - Plant Physiol. 118: 637-650, 1998. Go to original source...
    12. Kurepa, J., Van Montagu, M., Inzé, D.: Expression of sodCp and sodB genes in Nicotiana tabacum: Effects of light and copper excess. - J. exp. Bot. 48: 2007-2014, 1997.
    13. Perl-Treves, R., Galun, E.: The tomato Cu/Zn superoxide dismutase genes are developmentally regulated and respond to light and stress. - Plant mol. Biol. 17: 745-760, 1991. Go to original source...
    14. Pilon, M., Abdel-Ghany, S.E., Cohu, C.M., Gogolin, K.A., Ye, H.: Copper cofactor delivery in plant cells. - Curr. Opin. Plant Biol. 9: 256-263, 2006. Go to original source...
    15. Rao, M.V., Paliyath, G., Ormrod, D.P.: Ultraviolet-B and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. - Plant Physiol. 110: 125-136, 1996. Go to original source...
    16. Ruzsa, S.M., Scandalios, J.G.: Altered Cu metabolism and differential transcription of Cu/ZnSod genes in a Cu/ZnSOD deficient mutant of maize: evidence for a Cu-responsive transcription factor. - Biochemistry 42: 1508-1516, 2003. Go to original source...
    17. Schröppel-Meier, G., Kaiser, W.M.: Ion homeostasis in chloroplasts under salinity and mineral deficiency. I. Solute concentrations in leaves and chloroplasts from spinach plants under NaCl or NaNO3 salinity. - Plant Physiol. 87: 822-827, 1988. Go to original source...
    18. Trindade, L.M., Horvath, B.M., Bergervoet, M.J., Visser, R.G.: Isolation of a gene encoding a copper chaperone for copper/zinc superoxide dismutase and characterization of its promoter in potato. - Plant Physiol. 133: 618-629, 2003. Go to original source...
    19. Van Camp, W., Inzé, D., Van Montagu, M.: The regulation and function of tobacco superoxide dismutases. - Free Radical. Biol. Med. 23: 515-520, 1997. Go to original source...
    20. Wang, W.X., Vinocur, B., Shoseyov, O., Altman, A.: Biotechnology of plant osmotic stress tolerance: physiological and molecular considerations. - Acta Hort. 560: 285-92, 2001. Go to original source...
    21. Wintz, H., Vulpe, C.: Plant copper chaperones. - Biochem. Soc. Trans. 30: 732-735, 2002. Go to original source...
    22. Wintz, H., Fox, T., Wu, Y., Feng, V., Chen, W., Chang, H., Zhu, T., Vulpe, C.: Expression profiles of Arabidopsis thaliana in mineral deficiencies reveal novel transporters involved in metal homeostasis. - J. biol. Chem. 278: 47644-47653, 2003. Go to original source...
    23. Wintz, H., Vulpe, C.: Plant copper chaperones. - Biochem. Soc. Trans. 30: 732-735, 2002. Go to original source...
    24. Yu, Q., Rengel, Z.: Drought and salinity differentially influence activities of superoxide dismutase in narrow leafed lupins. - Plant Sci. 142: 1-11, 1999. Go to original source...

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