Biologia plantarum 55:269-278, 2011 | DOI: 10.1007/s10535-011-0038-2

Organ-specific effects of dark treatment on photosynthesis and the expression of photosynthesis-related genes

K. Mishev1, E. D. Ananiev2, K. Humbeck3,*
1 Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
2 Faculty of Biology, St. Kl. Ohridsky University of Sofia, Sofia, Bulgaria
3 Institute of Biology/Plant Physiology, Martin-Luther University Halle-Wittenberg, Halle, Germany

The effects of two-day dark treatment, applied to whole plants or to individual organs, on the photosynthetic apparatus in cotyledons and first rosette leaves of young Arabidopsis thaliana plants were studied. Darkness affected the individually darkened pair of cotyledons as well as the cotyledons of whole darkened plants (DP) in a similar manner as revealed by the significant decrease in the actual yield of photosystem 2 electron transport and the down-regulation of the psaB and rbcL transcript levels. However, cotyledons and rosette leaves responded differently to darkness with respect to the non-photochemical quenching (NPQ) and the non-regulated energy dissipation (ΦNO), indicating different capacity for photoprotection depending on the type of the applied dark treatment. Besides, the expression of the genes for the two plastid proteases FtsH5 and Deg1 involved in D1 protein degradation was inhibited in both leaf organs, suggesting that these proteases function mainly under irradiance. Upon re-irradiation, dark-treated cotyledons recovered from the applied stress and during further senescence the changes in the photosynthetic parameters and the mRNA levels of psaB, rbcL and SAG12 were similar as in the control plants. However, in the course of recovery typical chloroplast senescence symptoms were observed only in individually darkened leaves while re-irradiated DP leaves maintained high photosynthetic capacity.

Keywords: cotyledons; photosystem 2; plastid protease; rosette leaves; senescence

Received: May 15, 2009; Accepted: April 24, 2010; Published: June 1, 2011Show citation

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Mishev, K., Ananiev, E.D., & Humbeck, K. (2011). Organ-specific effects of dark treatment on photosynthesis and the expression of photosynthesis-related genes. Biologia plantarum, 55(2), 269-278. doi: 10.1007/s10535-011-0038-2.
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