Biologia plantarum 56:395-400, 2012 | DOI: 10.1007/s10535-012-0106-2

Genetic variability in regenerated plants of Ungernia victoris

O. M. Bublyk1, I. O. Andreev1,*, K. V. Spiridonova1, V. A. Kunakh1
1 Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv, Ukraine

To determine the suitability of micropropagation techniques developed for conserving rare medicinal herb Ungernia victoris we estimated the genetic fidelity of plants produced through direct regeneration from the bulb scale segments and organogenesis from long-term callus culture. Average value of the Jaccard's distances between explant-derived regenerants and maternal plants calculated from RAPD data was 0.5 %, while that of estimated between callus-derived regenerants and maternal cell line was 4.2 %; average distances between the objects among the explant-derived and callus-derived regenerants were 0.7 % and 2.5 %, respectively. The data obtained suggest that conditions for in vitro culture applied in this work provide relatively high genetic stability of the species upon the direct regeneration in vitro and regeneration from the long-term cultured callus.

Keywords: micropropagation; RAPD-analysis; somaclonal variation; tissue culture
Subjects: micropropagation; somaclonal variation; RAPD; in vitro culture
Species: Ungernia victoris

Received: July 26, 2010; Accepted: February 24, 2011; Published: June 1, 2012Show citation

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Bublyk, O.M., Andreev, I.O., Spiridonova, K.V., & Kunakh, V.A. (2012). Genetic variability in regenerated plants of Ungernia victoris. Biologia plantarum, 56(2), 395-400. doi: 10.1007/s10535-012-0106-2.
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    References

    1. Al-Zahim, M.A., Ford-Lloyd, B.V., Newbury, H.J.: Detection of somaclonal variation in garlic (Allium sativum L.) using RAPD and cytological analysis. - Plant Cell Rep. 18: 473-477, 1999. Go to original source...
    2. Arnholdt-Schmitt, B.: Genomic instabilities in tissue culture - a physiological normality? - In: Bender, L., Kumar, A. (ed.): From Soil to Cell - a Broad Approach to Plant Life. 12. Giessener Elektronische Bibliothek, Giessen 2001.
    3. Bohanec, B., Jakse, M., Ihan, A., Jovorik, B.: Studies in gynogenesis in onion (Allium cepa L.) - induction procedures and genetic analysis of regenerants. - Plant Sci. 104: 215-224, 1995. Go to original source...
    4. Bublyk, O.M., Andreev, I.O., Spiridonova, E.V., Kunakh, V.A.: [Somaclonal variability of U. victoris: the necessity for comprehensive genetic analysis.] - Biopolym. Cell 24: 487-493, 2008. [In Ukr.] Go to original source...
    5. Bublyk, O.M., Andreev, I.O., Spiridonova, E.V., Kunakh, V.A.: [Variability of Ungernia victoris morphogenic and nonmorphogenic tissue culture as results from RAPD-analysis.] - Bull. Vavilov Soc. Genet. Breed. Ukraine 6: 44-51, 2008. [In Ukr.]
    6. De la Puente, R., González, A.I., Ruiz, M.L., Polanco, C.: Somaclonal variation in rye (Secale cereale L.) analyzed using polymorphic and sequenced AFLP markers. - In Vitro cell. dev. Biol. Plant. 44: 419-426, 2008. Go to original source...
    7. Draper, J., Scott, R., Armitage, P., Walden, R.: Plant Genetic Transformation and Gene Expression. A Laboratory Manual. - Blackwell Scientific Publications, Oxford 1988.
    8. Gostimsky, S.A., Kokaeva, Z.G., Konovalov, F.A.: Studying plant genome variation using molecular markers. - Russ. J. Genet. 41: 378-388, 2005. Go to original source...
    9. Guo, W.L., Gong, L., Ding, Z.F., Li, Y.D., Li, F.X., Zhao, S.P., Liu, B.: Genomic instability in phenotypically normal regenerants of medicinal plant Codonopsis lanceolata Benth. et Hook. f., as revealed by ISSR and RAPD markers. - Plant Cell Rep. 25: 896-906, 2006. Go to original source...
    10. Gupta, P.K., Varshney, R.K.: Molecular markers for genetic fidelity during micropropagation and conservation. - Curr. Sci. 76:1308-1310, 1999.
    11. Gupta, P.K.: Chromosomal basis of somaclonal variation in plants. - In: Jain, S.M., Brar, D.S., Alhoowalia, B.S. (ed.): Somaclonal Variation and Induced Mutations in Crop Improvement. Pp. 149-168. Kluwer Academic Publishers, Dordrecht - Boston - London 1998. Go to original source...
    12. Huang, W.J., Ning, G.G., Liu, G.F., Bao, M.Z.: Determination of genetic stability of long-term micropropagated plantlets of Platanus acerifolia using ISSR markers. - Biol. Plant. 53: 159-163, 2009. Go to original source...
    13. Khamidkhodzhaev, S.A.: Lekarstvennye Rasteniya Roda Ungernia v Sredney Azii. [Medicinal Plants of the Ungernia Genus in Central Asia]. - FAN, Tashkent 1982. [In Russ.]
    14. Kozyrenko, M.M., Artyukova, E.V., Boltenkov, E.V., Lauve, L.S.: [Somaclonal variability of Iris pseudacorus L. judged by RAPD- and cytogenetic analyses.] - Russ. J. Biotechnol. 2: 13-23, 2004. [In Russ.]
    15. Kunakh, V.A.: Biotekhnologiya Likars'kykh Roslyn. Genetychni ta Fiziologo-Biokhimichni Osnovy [Biotechnology of medical plants. Genetic, physiological and biochemical basis] - Logos, Kyiv 2005. [In Ukr.]
    16. Kunakh, V.A., Mozhylevska, L.P., Bublyk, O.M., Kolonina, I.V., Muzyka, V.I.: [Microclonal propagation of Ungernia victoris Vved. ex Artjuschenko.] - Biotechnology (Kiev) 1: 57-63, 2008. [In Ukr.]
    17. Kunakh, V.A., Mozhylevska, L.P., Potapchuk, O.A., Muzyka, V.I., Kolonina, I.V.: [Obtaining of the Ungernia victoris tissue culture and its peculiarities upon growing in the nutrient media of different composition.] - Russ. J. Biotechnol. 1: 14-21, 2007. [In Russ.]
    18. Kushnir, G.P., Sarnats'ka, V.V.: Mikroklonal'ne Rozmnozhennya Roslyn. [Microclonal Propagation of Plants.] - Naukova Dumka, Kyiv 2005. [In Ukr.]
    19. Kuznetsova, O.I., Ash, O.A., Gostimsky, S.A.: [The effect of the duration of callus culture on the accumulation of genetic alterations in pea Pisum sativum L.] - Russ. J. Genet. 42: 555-562, 2006. [In Russ.] Go to original source...
    20. Limanton-Grevet, A., Sotta, B., Brown, S., Jullien, M.: Analysis of habituated embryogenic lines in Asparagus officinalis L.: growth characteristics, hormone content and ploidy level of calli and regenerated plants. - Plant Sci. 160: 15-26, 2000. Go to original source...
    21. Linacero, R., Freitas Alves, E., Vazques, A.M.: Hot spots of DNA instability revealed through the study of somaclonal variation in rye. - Theor. appl. Genet. 100: 506-511, 2000. Go to original source...
    22. Mo, X.Y., Long, T., Liu, Z., Lin, H., Liu, X.Z., Yang, Y.M., Zhang, H.Y.: AFLP analysis of somaclonal variations in Eucalyptus globulus. - Biol. Plant. 53: 741-744, 2009. Go to original source...
    23. Osipova, E.S., Kokaeva, Z.G., Troitskij, A.V., Dolgikh, Yu.I., Shamina, Z.B., Gostimskij, S.A.: [RAPD analysis of maize somaclones.] - Russ. J. Genet. 37: 80-84, 2001. [In Russ.] Go to original source...
    24. Osipova, E.S., Koveza, O.V., Troitskij, A.V., Dolgikh, Yu.I., Shamina, Z.B., Gostimskij, S.A.: [Analysis of specific RAPD and ISSR fragments in maize (Zea mays L.) somaclones and development of SCAR markers on their basis.] - Russ. J. Genet. 39: 1412-1419, 2003. [In Russ.] Go to original source...
    25. Polanco, C., Ruiz, M.L.: AFLP analysis of somaclonal variation in Arabidopsis thaliana regenerated plants. - Plant Sci. 162: 817-824, 2002. Go to original source...
    26. Rani, V., Raina, S.N.: Genetic fidelity of organized meristemderived micropropagated plants: a critical reappraisal. - In Vitro cell. dev. Biol. Plant. 36: 319-330, 2000. Go to original source...
    27. Rout, G.R., Samantaray, S., Das, P.: In vitro manipulation and propagation of medicinal plants. - Biotechnol. Adv. 18: 91-120, 2000. Go to original source...
    28. Schluter, P.M., Harris, S.A.: Analysis of multilocus fingerprinting data sets containing missing data. - Mol. Ecol. Notes. 6: 569-572, 2006. Go to original source...
    29. Smykal, P., Valledor, L., Rodriguez, R., Griga, M.: Assessment of genetic and epigenetic stability in long-term in vitro shoot culture of pea (Pisum sativum L.). - Plant Cell Rep. 26: 1985-1998, 2007.
    30. Soniya, E.V., Banerjee, N.S., Das, M.R.: Genetic analysis of somaclonal variation among callus-derived plants of tomato. - Curr. Sci. 80: 1213-1215, 2001.
    31. Tyagi, P., Khanduja, S., Kothari, S.L.: In vitro culture of Capparis decidua and assessment of clonal fidelity of the regenerated plants. - Biol. Plant. 54: 126-130, 2010. Go to original source...
    32. Varshney, A., Lakshmikumaran, M., Srivastava, P.S., Dhawan, V.: Establishment of genetic fidelity of in vitro-raised Lilium bulblets through RAPD markers. - In Vitro cell. dev. Biol. Plant. 37: 227-231, 2001. Go to original source...
    33. Vollosovich, A.G., Puchinina, G.M., Nikolaeva, L.A.: [Optimization of macrosalt composition for tissue culture of Rauwolfia serpentina Benth.] - Rast. Resursy 15: 516-526, 1979. [In Russ]
    34. Wilhelm, E., Hristoforoglu, K., Fluch, S., Burg, K.: Detection of microsatellite instability during somatic embryogenesis of oak (Quercus robur L.). - Plant Cell Rep. 23: 790-795, 2005. Go to original source...
    35. Xing, Y., Yu, Y., Luo, X., Zhang, J.-N., Zhao, B., Guo, Y.-D.: High efficiency organogenesis and analysis of genetic stability of the regenerants in Solanum melongena. - Biol. Plant. 54: 231-236, 2010. Go to original source...
    36. Yuan, X.F., Dai, Z.H., Wang, X.D., Zhao, B.: Assessment of genetic stability in tissue-cultured products and seedlings of Saussurea involucrata by RAPD and ISSR markers. - Biotechnol. Lett. 31: 1279-1287, 2009. Go to original source...

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