Biologia plantarum 58:531-538, 2014 | DOI: 10.1007/s10535-014-0417-6

Involvement of the vacuolar processing enzyme γVPE in response of Arabidopsis thaliana to water stress

A. Albertini1,2, F. Simeoni3,4, M. Galbiati1,3, H. Bauer5, C. Tonelli1,3, E. Cominelli6,*
1 Dipartimento di Bioscienze, Università degli Studi di Milano, Milano, Italy
2 Institute of Inflammation and Repair, University of Manchester, Manchester, UK
3 Fondazione Filarete, Milano, Italy
4 Dipartimento di Scienze Agrarie e Ambientali - Produzione, Territorio, Agroenergia, Università degli Studi di Milano, Milano, Italy
5 Julius-von-Sachs-Institut für Biowissenschaften, Universität Würzburg, Biozentrum, Würzburg, Germany
6 Istituto di Biologia e Biotecnologia Agraria, CNR, Milano, Italy

Plant vacuoles play several roles in controlling development, pathogen defence, and stress response. γVPE is a vacuolarlocalised cysteine protease with a caspase-1 like activity involved in the activation and maturation of downstream vacuolar hydrolytic enzymes that trigger hypersensitive cell death and tissue senescence. This work provides evidence that γVPE is strongly expressed in Arabidopsis guard cells and is involved in water stress response. The γvpe knock-out mutants showed reduced stomatal opening and an increased resistance to desiccation suggesting a new role of γVPE in control of stomatal movements.

Keywords: abscisic acid; guard cells; knock-out mutant; RT-qPCR; relative water content; stomata
Subjects: vacuolar processing enzymes; abscisic acid; stomata; guard cells; mutants; relative water content; stomatal density; gene expression; water stress

Received: June 4, 2013; Revised: December 17, 2013; Accepted: January 24, 2014; Published: September 1, 2014Show citation

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Albertini, A., Simeoni, F., Galbiati, M., Bauer, H., Tonelli, C., & Cominelli, E. (2014). Involvement of the vacuolar processing enzyme γVPE in response of Arabidopsis thaliana to water stress. Biologia plantarum, 58(3), 531-538. doi: 10.1007/s10535-014-0417-6.
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