Biologia plantarum 58:733-742, 2014 | DOI: 10.1007/s10535-014-0435-4

Evaluation of amino acid profile in contrasting arsenic accumulating rice genotypes under arsenic stress

A. Kumar1,2, S. Dwivedi1, R. P. Singh2, D. Chakrabarty1, S. Mallick1, P. K. Trivedi1, B. Adhikari3, R. D. Tripathi1,*
1 CSIR-National Botanical Research Institute, Lucknow, India
2 Department of Environmental Science, B.B.A. University, Lucknow, India
3 Rice Research Station, Department of Agriculture, Government of West Bengal, Chinsurah, India

Amino acids (AAs) play significant roles in metal binding, antioxidant defense, and signaling in plants during heavy metal stress. In the present study, the essential amino acids (EAAs), non-essential amino acids (NEAAs), as well as the enzymes of proline and cysteine biosynthetic pathways were studied in contrasting arsenic accumulating rice genotypes grown in hydroponic solutions with addition of arsenate (AsV) or arsenite (AsIII). Under a mild As stress, the total AAs content significantly increased in both the rice genotypes with a greater increase in a low As accumulating rice genotype (LAARG; IET-19226) than in a high As accumulating rice genotype (HAARG; BRG-12). At the equimolar concentration (10 μM), AsIII had a greater effect on EAAs than AsV. Conversely, AsV was more effective in inducing a proline accumulation than AsIII. Among NEAAs, As significantly induced the accumulation of histidine, aspartic acid, and serine. In contrast, a higher As concentration (50 μM) reduced the content of most AAs, the effect being more prominent during AsIII exposure. The inhibition of glutamate kinase activity was noticed in HAARG, conversely, serine acetyltransferase and cysteine synthase activities were increased which was positively correlated with the cysteine synthesis.

Keywords: arsenate; arsenite; cysteine synthase; glutamate kinase; Oryza sativa; proline; serine acetyltransferase
Subjects: amino acids; arsenic; cysteine synthase; glutamate kinase; proline; rice

Received: July 23, 2013; Revised: January 16, 2014; Accepted: February 5, 2014; Published: December 1, 2014Show citation

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Kumar, A., Dwivedi, S., Singh, R.P., Chakrabarty, D., Mallick, S., Trivedi, P.K., Adhikari, B., & Tripathi, R.D. (2014). Evaluation of amino acid profile in contrasting arsenic accumulating rice genotypes under arsenic stress. Biologia plantarum, 58(4), 733-742. doi: 10.1007/s10535-014-0435-4.
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