Biologia plantarum 59:227-236, 2015 | DOI: 10.1007/s10535-015-0500-7

Genomic structure and characterization of a lipase class 3 gene and promoter from oil palm

A. W. Nurniwalis1,3,*, R. Zubaidah1, A. Siti Nor Akmar2, H. Zulkifli1, M. A. Mohamad Arif1, F. J. Massawe3, K. L. Chan1, G. K. A. Parveez1
1 Persiaran Institute, Bandar Baru Bangi, Malaysian Palm Oil Board, No 6, Kajang Selangor, Malaysia
2 Faculty of Agriculture and Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
3 School of Biosciences, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih, Selangor, Malaysia

Lipase class 3 is part of the triacylglycerol lipase family involved in lipid degradation, esterification, and transesterification processes in plants. In this study, a lipase class 3 gene and promoter from oil palm (Elaeis guineensis Jacq.) were isolated and characterized by Northern blot, Southern blot, oil palm genome sequence, and transient expression GUS assay. The full-length lipase class 3 (FLL1) deduced polypeptide encoded 483 amino acids and was identical to that deduced from lipase (EgLip1) cDNA (GI: 409994625). It contained the lipase consensus sequence, GxSxG motif, and a putative catalytic triad and had a 3-dimensional protein model similar to that of a lipase from Giberella zeae with a 50 % identity. The Northern blot and reverse transcription polymerase chain reaction (RT-PCR) show that FLL1 was predominantly expressed in the mesocarp and the expression increased as fruits reached maturity. A lower expression was detected in germinated seedlings and especially in roots. The expression of FLL1 was also enhanced in the mesocarp of cold treated fruits. A high oil accumulation in the mesocarp during fruit development makes this tissue a suitable target for a genetic modification, hence the isolation of the FLL1 promoter. The transient expression of the β-glucuronidase (GUS) gene driven by the FLL1 promoter detected the GUS expression in mesocarp slices, especially in vascular bundles. This suggests the potential role of using the promoter as tool to direct the expression of a transgene to the mesocarp of transgenic oil palm.

Keywords: fatty acids; GUS gene; mesocarp; RACE; transient assay; triacylglycerides
Subjects: lipase class 3 gene; gene promoter; fatty acids; mesocarp; GUS expression; protein model; Southern blot; gene expression; oil palm
Species: Elaeis guineensis

Received: April 21, 2014; Revised: October 28, 2014; Accepted: October 30, 2014; Published: June 1, 2015Show citation

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Nurniwalis, A.W., Zubaidah, R., Siti Nor Akmar, A., Zulkifli, H., Arif, M.A., Massawe, F.J., Chan, K.L., & Parveez, G.K.A. (2015). Genomic structure and characterization of a lipase class 3 gene and promoter from oil palm. Biologia plantarum, 59(2), 227-236. doi: 10.1007/s10535-015-0500-7.
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