Biologia plantarum 58:39-46, 2014 | DOI: 10.1007/s10535-013-0379-0

Antigibberellin-induced reduction of internode length favors in vitro flowering and seed-set in different pea genotypes

F. M. Ribalta1,2, J. S. Croser1, W. Erskine1, P. M. Finnegan2, M. M. Lulsdorf3, S. J. Ochatt4,*
1 Centre for Legumes in Mediterranean Agriculture, Faculty of Natural and Agricultural Sciences, University of Western Australia, Crawley, Australia
2 School of Plant Biology, Faculty of Natural and Agricultural Sciences, University of Western Australia, Crawley, Australia
3 Crop Development Centre, University of Saskatchewan, Saskatoon, Canada
4 Institut National de la Recherche Agronomique, UMRLEG, PCMV, INRA C.R de Dijon, Dijon Cedex, France

In vitro flowering protocols were developed for a limited number of early flowering pea (Pisum sativum L.) cultivars. This work was undertaken to understand the mechanisms regulating in vitro flowering and seed-set across a range of pea genotypes. Its final goal is to accelerate the generation cycle for faster breeding novel genotypes. We studied the effects of in vivo and in vitro applications of the antigibberellin Flurprimidol together with radiation of different spectral compositions on intact plants, plants with the meristem removed, or excised shoot tip explants. Based on our results, we present a simple and reliable system to reduce generation time in vitro across a range of pea genotypes, including mid and late flowering types. With this protocol, more than five generations per year can be obtained with mid to late flowering genotypes and over six generations per year for early to mid flowering genotypes.

Keywords: acceleration of development; Flurprimidol; gibberellins; spectral composition of radiation
Subjects: antigibberellin; growth; in vitro flowering; in vitro seed-set; pea; Flurprimidol; gibberellins; irradiance

Received: February 5, 2013; Revised: July 30, 2013; Accepted: July 31, 2013; Published: March 1, 2014Show citation

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Ribalta, F.M., Croser, J.S., Erskine, W., Finnegan, P.M., Lulsdorf, M.M., & Ochatt, S.J. (2014). Antigibberellin-induced reduction of internode length favors in vitro flowering and seed-set in different pea genotypes. Biologia plantarum, 58(1), 39-46. doi: 10.1007/s10535-013-0379-0.
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