Biologia plantarum 55:708, 2011 | DOI: 10.1007/s10535-011-0172-x

Development of thirty new polymorphic microsatellite primers for Paeonia suffruticosa

X. -G. Hou1, D. -L. Guo2,*, S. -P. Cheng1, J. -Y. Zhang2
1 College of Agriculture, Henan University of Science and Technology, Luoyang, Henan Province, P.R. China
2 College of Forestry, Henan University of Science and Technology, Luoyang, Henan Province, P.R. China

Four new microsatellite primer pairs were developed in tree peony (Paeonia suffruticosa) based on the database mining and other twenty-six primer pairs by fast isolation by AFLP of sequences containing repeats (FIASCO) method. The polymorphism of each locus was further evaluated in 40 individuals of one population plus 5 tree peony related species. The number of alleles per locus ranged from 3 to 7 and the expected (He) and observed (Ho) heterozygosity at each locus ranged from 0.42 to 0.78 and 0.28 to 0.59, respectively. These microsatellite markers will be useful for investigating genetic diversity and studies of population genetic structure of tree peony.

Keywords: database mining; FIASCO; SSR; tree peony

Received: November 11, 2009; Accepted: April 15, 2010; Published: December 1, 2011Show citation

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Hou, X.-G., Guo, D.-L., Cheng, S.-P., & Zhang, J.-Y. (2011). Development of thirty new polymorphic microsatellite primers for Paeonia suffruticosa. Biologia plantarum, 55(4), 708. doi: 10.1007/s10535-011-0172-x.
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