Book reviews

J. Pozděna, Danuše Hodáňová, Libuše Pavlová, T. Gichner, J. Velemínský, Ingrid Tichá, P. Tichý, Jana Pospíšilová, Ingrid Tichá, M. Kaše, J. Bříza, A. Kamenická

Biologia plantarum 25:74-80, 1983 | DOI: 10.1007/BF02878270

Diurnal variations in leaf water potential and stomatal conductance of pigeon pea (Cajanus cajan (L.) Millsp.) cultivars as affected by irrigation levels

K. P. Singh, R. S. Malik, D. S. Malik

Biologia plantarum 25:1-4, 1983 | DOI: 10.1007/BF02878259

Two cultivars of pigeon pea (Cajanus cajan (L.)Millsp.) UPAS-120 and Parbhat were grown in the field under two irrigation treatments: no irrigation and irrigation when cumulative pan evaporation was equal to 50 per cent depletion of available water content in one metre root zone depth. Diurnal changes in leaf water potential and stomatal conductance were recorded on two daysi.e. October 1, 1979 and October 28, 1979 which corresponded to reproductive growth stage of the crop. Plant water potential decreased rapidly during the day up to about 15.00 and increased during evening hours. Higher leaf water potential was recorded in irrigated...

Regulation of androgenesis inNicotiana tabacum L. cv. White Burley andDatura innoxia Mill. Effect of bivalent and trivalent iron and chelating substances

J. Vagera, P. Havránek

Biologia plantarum 25:5-14, 1983 | DOI: 10.1007/BF02878260

The effect of FeSO₄.7H₂O, Fe₂(SO₄)₃.9H₂O, disodium salt of ethylene-diaminotetraacetic acid, dihydrate (EDTA) and N-(2-acetamido) iminodiacetic acid (ADA) and their combinations on the androgenesis was studiedin vitro in tobacco (cv. White Burley) and datura (Datura innoxia Mill.). Simultaneously the reversibility and irreversibility of the morphogenic process leading to the conversion of the pollen embryoid into complete plant was followed.Complete plants developed in anthers on media with trivalent iron, chelated trivalent iron, chelated bivalent iron, bivalent iron...

Legumin and albumin of pea cotyledons during seed germination

Ligia Konopska

Biologia plantarum 25:15, 1983 | DOI: 10.1007/BF02878261

During 6 days of pea seed germination the depletion of legumin with mol. m. 390 000 from protein bodies was observed. SDS-PAGE indicated that the legumin subunits with mol. m. 41 700 and 21 000 were prevailing. Only the former of these, probably corresponding to α-subunit, was degraded rapidly during 6 days of germination.Water-soluble proteins (albumins) prepared from pea cotyledons were separated by preparative IEF into proteins with pI 7.1, 6.5, 6.0, 5.4, 5.0, and 4.6. During 6 days the components of albumin with pI 7.1, and 6.5 were dramatically depleted. Major fractions with pI 6.5, 6.0, and 5.4 were subjected to SDS-PAGE and their subunit...

Role of inorganic nitrogen in the synthesis and degradation of chlorophyll and carotenoids in maize leaves

S. N. Mishra, H. S. Srivastava

Biologia plantarum 25:21-27, 1983 | DOI: 10.1007/BF02878262

Supply of KNO₃, NH₄Cl or NH₄NO₃ to the maize seedlings increased total chlorophyll and carotenoids (over zero nitrogen control) amounts per fresh matter unit of the primary leaves. The increase was most apparent when the pigment level in control plants started declining,i.e. between days 14 and 16. Supply of inorganic nitrogen increased pigment synthesis in excised leaf segments from dark grown seedlings, although the increase during a 24 h incubation was not as high as with either glycine or glutamine. Salicylic acid at 0.01 to 1 mM concentration increased and at 10 mM lowered the pigment biosynthesis....

The effect of plant growth regulators on formation of crown gall tumors on potato tuber discs

M. Hrouda, M. Ondřej

Biologia plantarum 25:28-32, 1983 | DOI: 10.1007/BF02878263

The effect of several plant growth regulators on the number of tumors developing on potato tuber discs (Solatium tuberosum L. cv. Radka) inoculated withAgrobacterium tumefaciens, strain C 58 was studied. The plant growth regulators used in appropriate range of concentrations stimulated the formation of tumors byA. tumefaciens.Naphthaleneacetic acid (NAA) was most active in concentration of 10^-4 mg ml^-1. Kinetin gave a biphasic response with optimal promotions of tumor initiation at 10^-4 - 2 × 10^-3 mg ml^-1. High kinetin concentration (10^-1 mg ml^-1)...

Comparison of the sensitivity of the serological latex and ELISA tests

J. Polák

Biologia plantarum 25:33-35, 1983 | DOI: 10.1007/BF02878264

A sensitivity of the serological latex and ELISA tests were compared in carnation mottle virus diagnosis. For the latex test carnation mottle virus (CaMV) antiserum was sensibilized with latex suspension for RF-test. Sensibilized antiserum was used in 1: 200 dilution, as compared with fresh antiserum. For ELISA the γ-globuline fraction of antiserum was conjugated with alkaline phosphatase. The optimal dilution in both, CaMV fraction of antisera for coating of plates and γ-globuline-enzyme conjugate were in the ratio of 1: 500, 2 μg of antibodies in 1 ml. The dilution end point of carnation mottle virus in sap from carnation leaves was...

Use of esterase isoenzymes revealed by gel isoelectric focusing as an aid in chemotaxonomical study of the genusAllium

Věra Hadačová, Jiřina Švachulová, Eva Klozová, E. Hadao, Květa Pitterová

Biologia plantarum 25:36-42, 1983 | DOI: 10.1007/BF02878265

Eleven species of the genusAllium belonging to the sectionCepa, Phyllodolon, Rhizirideum, Melanocrommyum andAlliun ware investigated as to the presence of esterase isoenzymes by means of the gal isoelectric focusing which shows better resolution than polyaorylamide gel electrophoresis. In all examined species twenty six isoenzymes were found. The individual sections are well characterized by esterase isoenzymes revealed by this method, on the other hand, the differences, as shown by the Jaceard index, between the subganera are insignificant. Investigation of seven cultivars ofAllium cepa shows the isolated position of the...

The comparison of lectins from albumin complexes of certain representatives of the speciesPhaseolus vulgaris

Jiřina Švachulová, Věra Turková, Eva Klozová

Biologia plantarum 25:43-49, 1983 | DOI: 10.1007/BF02878266

Using biospecific chromatography on D-Glc-Separon-fetuin, lectins were isolated from seed albumin complexes of four cultivars ofPhaseolus vulgaris (Veltruská Saxa, Vainica Saavegra, Krupnaya sakharnaya, Olympia) andPhaseolus vulgaris ssp.aborigineus (wild form, considered to be one of the ancestors of cultivated beans). In the lectins isolated the agglutinating activity against human erythrocytes of the A, B, and O groups was estimated, as well as against trypsin-treated and non-treated rabbit erythrocytes. Further analyses involved their mitogenic activity against lymphocytes of murine spleen, their isoelectric points by isoelectric...

Floral transition as a sequence of growth changes in different components of the shoot apical meristem ofChenopodium rubrum

Frideta Seidlová, Hana Sádlíková

Biologia plantarum 25:50-62, 1983 | DOI: 10.1007/BF02878267

The changes in cell division rate were studied in different components of the shoot apex ofChenopodium rubrum during short-day photoperiodic induction and after the inductive treatments. Induced and vegetative apices were compared. Accumulation of metaphases by colchicine treatment was used to compare the mean cell cycle duration in different components of the apex. A direct method of evaluating the increase in cell number obtained by anticlinal or periclinal divisions was applied if the corresponding components of induced and non-induced apices had to be compared. The short-day treatment prolonged the cell cycle more in the peripheral zone...

Isolation of some strains ofCorynebacterium fascians (Tilford) Dowson in Czechoslovakia

Marie Ulrychová, Eva Petrů

Biologia plantarum 25:63-67, 1983 | DOI: 10.1007/BF02878268

Four strains of the phytopathogenic bacteriumCorynebacterium fascians (Tilford)Dowson were selected from our isolates and deposited in the Czechoslovak National Collection of Type Cultures (CNCTC) of the Institute of Hygiene and Epidemiology in Prague. Two very virulent (Cor 83/82 "UPR" and Cor 82/81 "UP") and one avirulent (Cor 81/80 "CP1b") pelargonium strains, producing acid from rhamnose, were isolated from fasciations onPelargonium zonale W. One avirulent celery strain ofC. fascians (Cor 80/80 "CF4a") was isolated from a root expiant ofApium graveolens L. growing on a nutrient mediumin vitro. Morphological, cultural,...

Water relations pattern of understorey species influenced by sunflecks

P. Eliáš

Biologia plantarum 25:68-74, 1983 | DOI: 10.1007/BF02878269

Water relations of plant cenopopulations of understorey species (7 herbaceous perennials, 1 shrub, and 1 tree seedling) growing in an oak-hornbeam forest in SW. Slovakia is markedly influenced by natural sunflecks moving on the forest floor, where stomatal conductance and transpiration rate are significantly higher than in shade areas. In the course of a day, the leaf stomatal conductance fluctuates, following irradiance of the leaf, from minimum in full shade to maximum in sunflecks, including intermediate values in transient conditions. Therefore, there is also a large variability in water saturation deficit and transpiration rate within the cenopopulations...