Influence of ACC and Ethephon on cell growth in etiolated lupin hypocotyls. dependence on cell growth state

A. Ortuño, J. A. Del Rio, J. L. Casas, M. Serrano, M. Acosta, J. Sanchez-Bravo

Biologia plantarum 33:81, 1991 | DOI: 10.1007/BF02897783

The possible implication of ethylene on the growth regulation of etiolated lupin hypocotyls was investigated. Excised hypocotyl sections from actively growing seedlings produced ethylene at a rate of 3 nmol h^-1 g^-1 min^-1. The rate of ethylene production was increased about 7 times when sections were treated with 10 mM 1-aminocyclopropane-1-carboxylic acid (ACC). Measurement of endogenous ACC showed that 95 % of total ACC (64.2 nmol g^-1 min^-1) corresponded to conjugated ACC.Treatments to intact seedlings with the ethylene precursor ACC, and the ethylene generating compound, 2-chloroethyl phosphonic...

Evaluation of the most adequate organ of reference for sap analysis in the tomato plant

M. E. Garcia López de Sá, P. Azuara

Biologia plantarum 33:91, 1991 | DOI: 10.1007/BF02897784

The most adequate organ of reference for sap analysis is studied during the phenological stage corresponding to the fructification of the second flower bud in the tomato plant grown in hydroponic culture with normal nutrient solution. Thus, the composition of the sap extracted from petioles of leaves in different stages of growth is studied.The results obtained indicate that the sap extracted from the petioles of fully grown young leaves is the best for achieving a nutritional study of the tomato plant.

Organ correlations and flowering in chenopodium rubrum L.

Libuše Pavlová, J. Krekule, Dagmar Součková

Biologia plantarum 33:97-104, 1991 | DOI: 10.1007/BF02897785

Correlations within a shoot ofChenopodium rubrum L. ecotype 374 grown under continuous light or photoperiodic flower induction were studied using surgical treatments. Removal of a single pair of shoot organs had a variety of effects depending on position: significant changes in the number of leaf pair on the main axis or in axillary buds and in the height of shoot apices; or no effect on the parameters scored. Flowering was not affected by any of the treatments carried out. Decapitation brought about a significant increase in the number of leaf pairs in axillary buds and flowering was inhibited in 8- and 9-d old plants. Flowering was not affected...

Effect of spikelet removal on the whole plant senescence of rice

R. I. Khan, M. A. Choudhuri

Biologia plantarum 33:105, 1991 | DOI: 10.1007/BF02897786

The rice (Oryza sativa L.) cultivar IET 1444 showed a nonsequential mode of senescence as evident from the decline in chlorophyll and protein of the flag and second leaves at the senescent stage. Removal of 50,75 and 100 % spikelets from the panicle of rice plant or emasculation of the panicle by hot water treatment induced the development of secondary branch from the axil of second leaf but 25 % removal had no effect. Similarly, removal of 75 and 100 per cent spikelets from the panicle of secondary branch induced tertiary branch development, while 25 and 50 per cent removal had no such effect. Similar treatments on tertiary branch had no effect on...

Interactive effects of exogenous growth regulators and artificial acid rain on cytokinins and gibberellins in maple seedlings

RADMILA ČÍŽKOVÁ

Biologia plantarum 33:115, 1991 | DOI: 10.1007/BF02897788

Vegetation experiments were conducted under greenhouse conditions in which long-term effects of simulated acid rain on the shoots, and short-term effects of acidified root medium on the roots of two maple species (Acer pseudoplatanus L. andAcer negundo L.) were studied. Both alternatives of the acidification stress were studied with respect to exogenous applications of auxins and gibberellins. Changes in seedling growth caused by acid rain were not marked, but changes in the levels of endogenous growth regulators were detected, both the long-term exposure ofAcer pseudoplatanus L. seedling shoots to acid rain and the short-term...

Reserve proteins in the developing seeds of xhaynaldoticum sardoum.

A. Capocchi, L. Galleschi, Isa Grilli, P. Meletti

Biologia plantarum 33:127, 1991 | DOI: 10.1007/BF02897789

In this study the pattern of storage protein deposition in the developing seeds of xHaynaldoticum sardoum was examined. The accumulation of the various protein classes did not differ greatly between the two lines tested (Culmo Pieno and Culmo Vuoto). Nevertheless, some differences in the deposition pattern were revealed by polyacrylamide gel electrophoresis. In particular, the mature seeds of the two lines presented different electrophoretograms for the prolamin and glutelin proteins. These patterns reflect the differences between the two lines, which could play a role in their ability to adapt to environmental changes.

Photoautotrophy established in multiple-shoot cultures of ruta graveolens

J. C. Leclerc, J. Creche

Biologia plantarum 33:135-144, 1991 | DOI: 10.1007/BF02897790

During the growth of multiple-shoot cultures of Ruta graveolens, oxygen and carbon dioxide exchanges were continuously and simultaneously measured. The shoots subcultured on a medium containing 166 mM glucose showed a marked respiration rate. Even under light, CO₂ concentrations reached 4000 to 6000 cm³ m^-3. Photosynthesis never compensated for respiration. These cultures were photomixotrophic. A change of respiration and photosynthesis occurred between the 30th and the 32nd day of culture, with a high respiration rate. When the shoots were subcultured on a medium containing 41 mM glucose, it was possible to obtain...

Chromosome localization of genes for soluble proteins in hexaploid wheat (triticum aestivum L.)

Ts. Stoilova, G. Ganeva, B. Bochev, K. Petkolicheva

Biologia plantarum 33:145-149, 1991 | DOI: 10.1007/BF02897791

An electrophoretic spectra of proteins, extracted with tris-HCI buffer, pH 8.3 are studied. The ditelosomic lines of the Chinese Spring common wheat cultivar are analysed by the chromosomes of the B genome and of the ditelosomic lines of the same cultivar by first and third chromosomes of the D genome. It is found that structural genes for the synthesis of components Nos. 7, 8, 9 and 10 are localized in 1BL, 2BS, 4BS and 5B chromosomes respectively. The genetic control of the component No. 3 is realized by genes, localized in 1BL and 3D chromosomes, while for component No. 2, in the 3D chromosome.

Subcellular localization of glucose-6-phosphate dehydrogenase in tobacco mesophyll protoplasts

Milada Šindelářová, L. Šindelář

Biologia plantarum 33:150, 1991 | DOI: 10.1007/BF02897792

Subcellular localization of glucose-6-phosphate dehydrogenase (EC 1.1.1.49.) isoenzymes was determined in mesophyll protoplasts prepared from Nicotiana tabacum L. cv. Samsun. Intact chloroplasts and soluble cytosolic proteins were obtained by means of differential centrifugation. The 1000 g pellet contained 97 % of chloroplasts and 16.8 ± 2.1 % of the total activity of glucose-6-phosphate dehydrogenase. The rest of the enzyme was localized in the cytosol which also contained 91 % of the total activity of phosphoenolpyruvate carboxylase.

Effects of the insecticide pyrethroid ii in the ames test, and onhordeum vulgare andvicia faba

Eva Miadoková, Marta Miklovičová, Viola Dúhová, Ľuba Garajová, Blanka Böhmová, Svetlana Podstavková, D. Vlček

Biologia plantarum 33:156-162, 1991 | DOI: 10.1007/BF02897794

The insecticide pyrethroid II, representing synthetic pyrethroids of the second generation, was not found to be mutagenic inSalmonella typhimurium strains TA1535, TA100, TA1538, TA98 and TA97. High concentrations of the tested insecticide inhibited the germination and length of roots of germinating seeds, the height of plants cultivatedin vitro, and slightly increased the frequency of aberrant anaphases and telophase in root-tips ofVicia faba andHordeum vulgare.

Long-term effect of irradiance on growth, water relations and epidermal conductance of two cyclamen cultivais

Jana Pospíšilová, Jarmila Solárová, F. Šrámek, M. Peisker

Biologia plantarum 33:163-167, 1991 | DOI: 10.1007/BF02897795

Decrease in leaf irradiance to 50 % due to shading of plants in glasshouse only during clear summer days did not induce significant changes in growth parameters, characteristics of water relations and epidermal conductance of two cyclamen cultivars. Thus the possibility of acclimation of plants to non-stable changes in environmental conditions was not proved.

Davis, T. D., Haissig, B. E., Sankhla, N. (ed.): Adventitious Root Formation in Cuttings. Advances in Plant Sciences Series. Volume 2. Advances in Plant Sciences Series. Volume 2

Biologia plantarum 33:114, 1991 | DOI: 10.1007/BF02897787

Magee, P. S., Henry, D. R., Block, J. H. (ed.): Probing Bioactive Mechanisms. A. C. S. Symposium Series

Miroslav Kaminek

Biologia plantarum 33:155, 1991 | DOI: 10.1007/BF02897793

Osborne, D. J., Jackson, M. B. (ed.):Cell Separation in Plants. Physiology, Biochemistry and Molecular Biology. NATO ASI Series, Ser. H: Cell Biology, Vol. 35

Ivana Macháčková, L. Fojtik

Biologia plantarum 33:168, 1991 | DOI: 10.1007/BF02897796

Erratum

Jarmila Solárová, Jana Pospisilova, M. Ondřej, M. Juřiček, T. Gichner, M. Ondřej, Th. Gaspar, V. Novák, Jarmila Solárová

Biologia plantarum 33:168, 1991 | DOI: 10.1007/BF02897797