Biologia plantarum 56:215-220, 2012 | DOI: 10.1007/s10535-012-0079-1

Cellular origin and development of secondary somatic embryos in Oncidium leaf cultures

J. -T. Chen1,*, P. -I. Hong2
1 Institute of Biotechnology, National University of Kaohsiung, Kaohsiung, Taiwan, Republic of China
2 Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China

We report here for the first time that fully differentiated stomatal guard cells have the ability to form directly secondary somatic embryos. Histological and scanning electron microscopy studies reveal that the stomata are formed on primary embryos of both Oncidium cultivars Gower Ramsey and Sweet Sugar. Secondary embryogenesis from these guard cells could be induced by several plant growth regulators (PGRs), including N6-benzyladenine, kinetin, thidiazuron, 1-aminocyclopropane-1carboxylic acid (ACC), ancymidol and 2,3,5-triiodobenzoic acid. When compared with other PGRs, ACC at concentration 1 mg dm-3 resulted in highest number of secondary embryos that were derived from guard cells. The present communication provides a model system for studying factors and mechanism affecting totipotency or embryogenetic capacity of guard cells.

Keywords: direct somatic embryogenesis; growth regulators; guard cell; morphogenetic pathway; orchid
Subjects: direct somatic embryogenesis; growth regulators; guard cell; morphogenetic pathway; in vitro culture; benzyladenine; kinetin; thidiazuron; stomata; leaf - epidermis; leaf - mesophyll; abscisic acid

Received: March 25, 2010; Accepted: November 18, 2010; Published: June 1, 2012Show citation

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Chen, J.-T., & Hong, P.-I. (2012). Cellular origin and development of secondary somatic embryos in Oncidium leaf cultures. Biologia plantarum, 56(2), 215-220. doi: 10.1007/s10535-012-0079-1.
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