Biologia plantarum, 1981 (vol. 23), issue 5

Article

Book Reviews

J. Čatský

Biologia plantarum 23:341, 1981 | DOI: 10.1007/BF02877409

Book review

T. Gichner, J. Ullmann, J. Slavík, Jarmila Solárová, I. Dvořák, J. Čatský, Marie Ulrychová

Biologia plantarum 23:397-400, 1981 | DOI: 10.1007/BF02877424

Original Papers

Gibberellic acid- and salicylic acid-caused formation of new proteins associated with extension growth and flowering ofImpatiens balsamina

Surinder Kumar, K. K. Nanda

Biologia plantarum 23:321-327, 1981 | DOI: 10.1007/BF02877404

GA3 as well as SA increase the protein content of the stem and the leaves at 1 day under both 8- and 24-h photoperiods. A new protein band with Rm 0.47 seems to be associated with floral bud initiation as it develops within 1-3 days in the stem as well as in the leaves of plants exposed to inductive treatments regardless of whether the induction is caused by 8-h photoperiods or by treatment with GA3 of SA under 24-h photoperiods. Another band with Rm 0.23 developed only in the stem of water-as well as GA3- or SA-treated plants under 8-h photoperiods. It may possibly be associated with extension growth.

Effect of gibberellic acid and salicylic acid on the activity and electrophoretic pattern of IAA-oxidase during floral induction inImpatiens balsamina

Surinder Kumar, K. K. Nanda

Biologia plantarum 23:328-334, 1981 | DOI: 10.1007/BF02877406

IAA-oxidase activity increased in the stem as well as in the leaves of plants treated with GA3, SA and GA3 + SA during the early stages under inductive and non-inductive photoperiods, the activity being the highest in GA3 + SA-treated plants. An isoenzyme of IAA-oxidase with Rm 0.15 developed in the stem as well as in the leaves subsequent to 1 or 2 inductive treatments. As this band persisted till the end of the experiment, it may be associated with the initiation as well as development of floral buds. Another band (Rm 0.30) appears to be associated with the phenol (SA) as it developed in the stem as well as in the...

Enzyme activity and electrophoretic pattern of isoenzymes of peroxidase, esterase and alkaline and acid phosphatase in relation to flowering inAmaranthus viridis L. - a quantitative SD plant

S. Sawhney, A. S. Basra, R. K. Kohli

Biologia plantarum 23:335-341, 1981 | DOI: 10.1007/BF02877408

Amaranthus viridis is a quantitative SD plant in which inflorescence primordia are initiated under both 24- and 8-h photoperiods after 12 and 10 days, when 8 and 7 leaves are differentiated, respectively. Photoperiod plays a non-determinate role, whereas the maturity of plants linked with the attainment of minimum leaf number is significant and of primary importance in floral induction. This is further confirmed by the more or less identical nature of changes in the total enzyme activity and isoenzyme patterns of peroxidase, esterase and alkaline and acid phosphatase under the two photoperiods. These changes occur once the minimum vegetative...

Identification of cytokinins present in root nodules ofPhaseolus mungo L

V. Jaiswal, S. J. H. Rizvi, D. Mukerji, S. N. Mathur

Biologia plantarum 23:342-344, 1981 | DOI: 10.1007/BF02877410

Root nodules ofPhaseolus mungo were extracted for their cytokinin content. Four cytokininsviz. zeatin, zeatin riboside, N6(‡2-isopentenyl) aminopurine and its riboside were identified on the basis of Rf values obtained by co-chromatography with authentic samples of cytokinins on thin layer of a mixture of polyvinylpyrrolidone/CaSO4.

Transaminase GOT and GPT activity in extirped sprouts of normal and opaque-2 Maize (Zea mays L.) seedlings

M. Ebeid, J. Eder, M. Kutáček, A. Piovarči

Biologia plantarum 23:345-350, 1981 | DOI: 10.1007/BF02877411

The increased activity of GOT (E.C.2.6.1.1.) and GPT (E.C.2.6.1.2.) transaminases in maize seedlings found as a marker of genotype opaque-2, was investigated in extirped sprouts of both genotypes, normal and opaque-2. The enzymatic activity was determined in three maize samples from breeding experiments, each sample consisting of a genotype pair, normal and opaque-2, collected from segregating ears of maize plants in the S1 generation. The seedlings were aseptically grown for 7 days in two variants of cultivation, intact seedlings and sprouts extirped after 4 days of germination.In the intact seedlings of genotype opaque-2 an increased...

Cell wall-bound trehalase in cultured cells of Japanese morning-glory

S. Hisajima, T. Hasegawa, T. Ito, T. Suzuki

Biologia plantarum 23:351-355, 1981 | DOI: 10.1007/BF02877413

Occurrence and distribution of trehalase were examined in cytoplasmic and cell wall fractions of cultured cells of morning-glory, soybean and persimmon. Also, some enzymatic properties and solubilization of the enzyme from cell walls were examined. Trehalase was present in both fractions of morning-glory and persimmon cells while trehalase was present only in the cytoplasmic fraction of soybean cells. Morning-glory trehalases in both fractions showed the same optimum pH at 5.5, while persimmon trehalases in both fractions showed the same optimum pH at 6.0. Soybean enzyme in the cytoplasmic fraction showed two optimum activities at 4.0 and 6.5.Morning-glory...

Purification and properties of sucrose synthetase from morning-glory callus cells

S. Hisajima, T. Ito

Biologia plantarum 23:356-364, 1981 | DOI: 10.1007/BF02877414

Sucrose synthetase was purified about 130-fold from morning-glory (Pharbitis nil Choisy cv. Murasaki) callus cells, and the properties of sucrose synthesis and cleavage activities of the enzyme were compared.The enzyme preparation gave a single band by disc electrophoresis. The molecular mass of the enzyme was estimated to be 4.2 × 105 by gel filtration. The enzyme preparation gave two bands by SDS disc electrophoresis, suggesting the molecular mass of about 3.8 ×104 and 7.0 × 104.The pH optima of sucrose synthesis and cleavage activities of the enzyme were different from each other, giving pH 9.0 and...

The uptake, distribution, and translocation of86Rb in alfalfa plants susceptible and resistant to the bacterial wilt and the effect ofCorynebacterium insidiosum upon these processes

I. Hanker, Anna Kdelová

Biologia plantarum 23:365-375, 1981 | DOI: 10.1007/BF02877415

Alfalfa (Medicago sativa; L.) plants susceptible (S) and resistant (R) to the bacterial wilt were fedvia roots with a nutrient solution labelled with86Rb+, at different times after inoculating them withCorynebacterium insidiosum (McCull.) H. L. Jens. The infection did not influence86Rb+ uptake per plant in the course of a 14-day-period following inoculation, however it did affect its distribution differentially in the S- and the R-plants.86Rb+ uptake was significantly decreased due to the infection in the S-plants on the day 49 after the inoculation (a 4-h-exposure...

Serological comparisons of seed proteins of some Allium (L.) species belonging to the subgenus Rhizirideum (G. Don ex Koch) Wendelbo

Eva Klozová, Vera Turková, Vera Hadačová, Jiřina Švachulová

Biologia plantarum 23:376-380, 1981 | DOI: 10.1007/BF02877417

Similarity of seed protein spectra of 13 representatives of the genusAllium, subgenusRhizirideum, was estimated by means of immunochemical analysis; the species investigated were divided into four groups more or less corresponding to the contemporary systematic division of species into sections.

Alfalfa witches' broom in Czechoslovakia and demonstration of mycoplasma-like organisms in the affected plants

J. Smrz, Marie Ulrychová, M. Jokeš

Biologia plantarum 23:381-383, 1981 | DOI: 10.1007/BF02877419

Alfalfa witches' broom manifested by an expressive increase in the number of stems and dwarfing of the affected plants was found in Czechoslovakia. The disease is not saptransmissible and was transmitted by grafting to healthy alfalfa plants. The disease occurrence is sporadic. The symptoms observed in comparison with those described in Australia and USA are mild and inconspicuous. Electron micrographs of ultrathin sections showed the presence of numerous mycoplasma-like organisms (MLO) in diseased but not in healthy phloem tissues.

Plant proteases as interfering factors in the electron microscopic detection of alfalfa mosaic and bean yellow mosaic viruses inSolanum laciniatum Ait

M. Čech, Š. Neubauer

Biologia plantarum 23:384-388, 1981 | DOI: 10.1007/BF02877420

Plant proteases rapidly destroy virus architecture in sap ofSolanum laciniatumAit., with yellow mosaic symptoms but even under these conditions the partially digested amorphous nucleoproteins retain their immunospecificity and infectivity for a limited time. Homogenization with phenylmethylsulfonylfluoride inhibits efficiently host serine proteases and fully stabilizes the virus particles in plant sap and in partial purificates. During the electronmicroscopic examination of stabilized infectious saps in 12 out of 14 isolates of yellow mosaic ofS. laciniatum besides alfalfa mosaic virus a poty-virus was disclosed. This was identified serologically...

Brief Communications

Irradiation induced changes in flower formation inCannabis sativa L

R. K. Nigam, Mary Varkey, D. E. Reuben

Biologia plantarum 23:389-391, 1981 | DOI: 10.1007/BF02877422

Stimulation of flower formation was ohserved in the female plants ofCannabis sativa raised from seeds treated with gamma rays. These plants produced seeds, of smaller size. One of 195 plants formed sterile hermaphrodite flowers with reduced female structures and increased number of anthers.

Book Review

Entwiklungsbiologie

K. Beneš

Biologia plantarum 23:327, 1981 | DOI: 10.1007/BF02877405

Physiological Ecology of the Alpine Timberline

B. Slavík

Biologia plantarum 23:334, 1981 | DOI: 10.1007/BF02877407

Nitrogen and Carbon Metabolism

Jarmila Solárová

Biologia plantarum 23:350, 1981 | DOI: 10.1007/BF02877412

Cell Compartmentation and Metabolic Channeling

Z. Opatrný

Biologia plantarum 23:375, 1981 | DOI: 10.1007/BF02877416

The Penguin Dictionary of Biology

J. Čatský

Biologia plantarum 23:380, 1981 | DOI: 10.1007/BF02877418

Ökologie der Hochgebirge

B. Slavik

Biologia plantarum 23:388, 1981 | DOI: 10.1007/BF02877421

Molecular Biology of Plants

J. Tupý

Biologia plantarum 23:391, 1981 | DOI: 10.1007/BF02877423