biologia plantarum

Biologia plantarum 64: 541-550, 2020 | DOI: 10.32615/bp.2020.062

MicroRNA profiling the resurrection plant Haberlea rhodopensis unveils essential regulators of survival under severe drought

E. APOSTOLOVA¹, M. GOZMANOVA¹, L. NACHEVA⁴, Z. IVANOVA², V. TONEVA^1,2, I. MINKOV^2,3, V. BAEV¹, G. YAHUBYAN^1*

¹ Department of Plant Physiology and Molecular Biology, University of Plovdiv, BG-4000 Plovdiv, Bulgaria

² Institute of Molecular Biology and Biotechnologies, BG-4108 Markovo, Bulgaria

³ Center of Plant Systems Biology and Biotechnology, BG-4000 Plovdiv, Bulgaria

⁴ Fruit Growing Institute - Plovdiv, BG-4000 Plovdiv, Bulgaria

Small RNAs (sRNAs) are essential components of gene-regulatory networks, which guide plant development and tune it to environmental challenges. Though the past years have witnessed evidences on sRNA importance for stress response, there is scarce data on their involvement in resurrection plant survival under severe drought. Haberlea rhodopensis (hrh) is an angiosperm resurrection species, whose vegetative tissues can tolerate desiccation and recover upon rehydration. In this study, high-throughput sequencing sRNAs indicated a higher complexity of the sRNA population, especially of a 24 nt sRNA category, in the desiccated vegetative tissue of H. rhodopensis compared to unstressed tissues. The cross-species discovery was performed to predict 77 mature microRNAs (miRNAs), most of which were assigned to 23 high-confidence conserved miRNA families in the leaf tissue. Several members of the miR156/157, miR166, and miR399 families were found to be desiccation-responsive. The miR156/157 family members were found up-regulated upon dehydration and down-regulated upon rehydration, while the miR166 and miR399 family members followed an opposite trend of expression. A probable miR156/157 target, orthologous to the SQUAMOSA promoter binding protein-like, was reconstructed in H. rhodopensis based on genomic data available for this species and the closely related Boea hygrometrica. Reverse transcription quantittative PCR analysis confirmed the expression profile of hrh-miR156a-5p and hrh-miR157-5p established by sRNA sequencing and revealed an inverse expression pattern between these miRNAs and their targets in the desiccated tissue. Our study suggests that the miR156/157 and miR399 families are essential for plant survival under severe drought due to their ability to control plant development and growth by modulating transcription factor expression.

Keywords: desiccation tolerance, small RNAs, regulatory network.

Received: January 22, 2020; Revised: March 26, 2020; Accepted: April 21, 2020; Published online: August 10, 2020Show citation