Biologia plantarum 64: 692-700, 2020 | DOI: 10.32615/bp.2020.096

Identification of potential key genes affecting soybean growth under salt stress via transcriptome study

N. LI1,2, Z. LI2, S. FAN2, Y. PU1, Y. GONG1, R. TIAN1, X. GUO2, H. DING1,2,*
1 Scientific Research Office, Shandong Center of Crop Germplasm Resources, 250100, Jinan, Shandong, P.R. China
2 College of Life Science, Shandong Normal University, 250014, Jinan, Shandong, P.R. China

Soybean is one of the most important economic crops in the world. However, the salinization of soil results in the decrease of soybean yield as it is only a moderately salt-tolerant crop. We treated three soybean cultivars with low and high concentrations of NaCl. The differentially expressed genes between the control group and the salt treatment group were identified by mRNA sequencing and analyzed by gene ontology and Kyoto encyclopedia of genes and genomes annotations. We performed weighted gene co-expression network analysis on all samples and found genes most related to the phenotype. After verifying the results of differentially expressed genes by quantitative PCR, we finally identified Glyma06G01990, Glyma08G22730, Glyma019G05140, and Glyma06G20160 as key genes affecting the soybean growth under salt stress.

Keywords: differentially expressed genes, Glycine max, RNA-seq, WGCNA.

Received: February 11, 2020; Revised: June 24, 2020; Accepted: June 25, 2020; Published online: October 6, 2020Show citation

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LI, N., LI, Z., FAN, S., PU, Y., GONG, Y., TIAN, R., GUO, X., & DING, H. (2020). Identification of potential key genes affecting soybean growth under salt stress via transcriptome study. Biologia plantarum, 64, 692-700. doi: 10.32615/bp.2020.096.
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